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Study on Endoparasite Perkinsns sp. in the Manila Clams Ruditapes Philippinarum in China

Author: LiangYuBo
Tutor: ZhangFuSui
School: Graduate School , Chinese Academy of Sciences ( Institute of Oceanography )
Course: Marine biology
Keywords: Ruditapes philippinarum Parkin insects Disease epidemics Detection methods
CLC: S944
Type: PhD thesis
Year: 2005
Downloads: 162
Quote: 3
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Abstract


The Parkin insects is one of the the shellfish most important pathogens. Of the the important economic shellfish Philippines Ruditapes parasitic Parkin insects follows a more systematic study: (1) 7 November 2000, the North Yellow Sea tidal flats maricultural Ruditapes philippinarum mass death . The results show that Ruditapes philippinarum parasitic Parkin worm infection test further determine the Parkin worm is the cause of his death; (2) Parkin to major parasitic insects trophozoites in Ruditapes philippinarum connective tissue in the gills , mantle, digestive gland, more small adductor muscle, water pipes; serious infection can lead to epithelial nuclear pyknosis, necrosis, loss; (3) from 2000 to 2003, China's Bohai Sea, Yellow Sea, East China Sea, 21 stations Ruditapes philippinarum vivo Parkin insect detection, results show that China's coastal waters Ruditapes philippinarum generally suffered the Parkin worm infections (; 4) from October 2000 to October 2001, on the North Yellow Sea Port , Shicheng Island, Ruditapes philippinarum Lingshui Bridge Parkin insects annual survey found that the seasonal variation of the number of Parkin insects is not very clear, slightly spring and autumn extent of infection is high, winter and summer low trend; (5) Indoor Experimental results show that Ruditapes philippinarum in natural seawater temperature Parkin insects content higher than that of natural seawater, higher than in the winter, and in the late spring and early summer when the water temperature reaches about 20 ℃ there is an obvious trend of increasing the number Ruditapes mortality in the Philippines along with significantly increased, showing obvious effects of warm winter; 20 to 24 of Ruditapes philippinarum Parkin insects breeding optimum seawater salinity; (6) Parkin worm infections, plus large Ruditapes philippinarum energy expenditure level, reduce the energy income levels; (7) rRNA gene fragment nucleotide sequence analysis confirm the Parkin of Ruditapes philippinarum parasitic insects Perkinsus sp. P.olseni (or P.atlanticus ); (8) Application commercialization medium 1:2 DME: Ham's F12 vitro pure culture of Ruditapes philippinarum Parkin insects; (9) the establishment of an indirect enzyme-linked immunosorbent assay (IELISA), this method of Ruditapes philippinarum Parkin worm detection, greatly reducing the detection time than the traditional detection of RFTM method can be completed within 24h. In summary, this study opens up new areas of research of China's economic shellfish Ruditapes philippinarum parasitic Parkin insects and diseases, it not only expands the horizons of our economy shellfish disease research and Parkin worm detection method science, biology, epidemiology research achievements for the growing of shellfish farming and disease control theory and application of knowledge.

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CLC: > Agricultural Sciences > Aquaculture, fisheries > Fisheries Protection > Shellfish diseases, predators,and its control
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