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Studies on Apoptosis and Bcl-2/Bax Gene Expression Induced by Somatostatin Analogue in Human Hepatoma Cell Line Hepg2

Author: ChenJun
Tutor: WuTaiZuo
School: Shandong University
Course: Surgery
Keywords: Somatostatin Octreotide Hepatocellular carcinoma Apoptosis Gene Expression
CLC: R735.7
Type: PhD thesis
Year: 2004
Downloads: 204
Quote: 0
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Abstract


Objective: Primary liver cancer (Primary liver cancer, PHC) is one of the world's most serious malignancy, each year about 125 million people lost their lives to this end. China is a high incidence of PHC. The third primary liver cancer in the incidence of malignant tumors in China, the annual incidence rate is about Europe and the United States more than 10 times. According to Ministry of Health statistics, liver cancer mortality for our male malignancies second female malignancy 4. Liver cells liver cancer (hepatocellular carcinoma, HCC) in China accounted for PHC vast majority (91.5%). Primary liver cancer is difficult to find, and development of fast, short survival and high mortality, and poor prognosis. Clinical attenders are mostly middle and late surgical resection rate, conventional chemotherapy and radiotherapy ineffective, and the side effects, the patient is often difficult to tolerate, other therapies such as gene therapy, biological therapy role is not obvious. In recent years, more and more attention somatostatin (somatostatin, SST) and its analogs on the inhibition of tumor. The clinical application of somatostatin has achieved satisfactory results in the treatment of neuroendocrine tumors. A large number of studies have shown that somatostatin and its analogues can inhibit the digestive system of solid tumors such as stomach cancer, colon cancer, pancreatic cancer growth, and that the inhibitory effect of somatostatin induced apoptosis of tumor cells (of apoptosis) . But less somatostatin inhibition of primary liver cancer. Octreotide (Octreotide), trade name Sandostatin (Sandostatin) is a synthetic somatostatin octapeptide derivative, has a long half-life, a more powerful role than natural somatostatin, more convenient and effective clinical applications. This research using flow cytometry, immunohistochemistry and in situ hybridization observed octreotide on hepatoma cells (HepG 2 ) apoptosis and gene explore the inhibitory effect of somatostatin on liver cancer The mechanism, to provide experimental basis for clinical application. Method: using octreotide (octreotide) (0.25μg/ml), respectively, to the role of human hepatoma cells (the HepG 2 ) 0h, 6h, 12h and 24h, 0h sample as a control group, using DNA agarose gel electrophoresis and flow cytometry to detect apoptosis; using immunohistochemistry and in situ hybridization to study not the same phase hepatoma cells (HepG 2 ) of bcl-2/bax expression changes. Results: octreotide HepG 2

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
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