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Studies on Stable and Ordered Multilayer (or Monolayer) Film Electrodes Containing Glucose Oxidase

Author: ZhangSuXia
Tutor: SunChangQing
School: Jilin University
Course: Analytical Chemistry
Keywords: Biosensor Multilayers Membrane electrode Gold nanoparticles Enzyme electrode Chemically modified electrode Bonding method Functional molecules Sensing performance Alternating deposition
CLC: O657.1
Type: PhD thesis
Year: 2005
Downloads: 458
Quote: 0
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Abstract


In recent years, chemically modified electrode based biosensor research and development of more and more widespread concern . Select the appropriate experimental methods, bio - functional molecules on the electrode surface , stable and orderly immobilized in order to achieve optimization of sensor performance is the focus of the study of the work of this thesis . Stability has been one of the main problems facing biosensor . Traditional covalent bonding method , although capable of stable enzyme immobilization , the experimental operation is time-consuming and laborious, but also cause loss of activity of the biomolecule . The second chapter two easy ways to successfully solves this problem : First , the use of mild Schiff reaction between the amine and aldehyde groups , aldehyde of glucose oxidase and amine - containing polymer alternately deposited on the gold electrode ; second , for the first time the use of in situ photochemical reaction between the layer electrostatic assembly of enzyme multilayer film by near - UV irradiation into covalent connection . The experimental results show that , these two methods to build the enzyme multilayer film having structural stability, and orderly space , while the enzymes in the multilayer film having good catalytic activity , the performance of the sensor can be assembled through the regulation of glucose oxidase the number of layers of control . This thesis also nanoparticles introduced into the construction of enzyme electrodes : one is the glucose oxidase immobilized in the surface of the nanoparticles , the use of Gold Nanoparticles with good electrical conductivity , and biocompatibility and a large specific surface area to improve the enzyme electron transfer ability of the electrode to increase the load amount of the enzyme , thereby enhancing the analytical performance of the biosensor ; second is to rely on electrostatic interaction of glucose oxidase multilayers containing Au particles penetrate into , the successful realization of the enzyme simple, rapid, multi-layered , orderly and stable immobilized .

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Electrochemical analysis
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