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Heat and dampness resistant molecular mechanism of liver fibrosis

Author: WenBin
Tutor: WuShiJiu
School: First Military Medical University
Course: Traditional Chinese Medicine
Keywords: Heat and dampness Liver fibrosis Apoptosis Cytokines Signal Transduction
CLC: R259
Type: PhD thesis
Year: 2005
Downloads: 224
Quote: 0
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Research Source: Bureau of Guangdong Province Traditional Chinese Medicine scroll funded projects ( 303,012 ) . Purpose and meaning : To investigate the heat and dampness antifibrotic molecular mechanism for the clinical application of heat and dampness provide a theoretical basis for the development of liver fibrosis medicine lay a solid theoretical foundation . Methods: a composite factor -induced rat model of liver fibrosis as the research object , observe the heat and dampness France ( on behalf of parties Qingxiangsan Yuka Ru , gall grass, Bupleurum , Scutellaria , Atractylodes, Poria, hawthorn , Dan, red Shao etc. ) on the model rats. 50 Wistar rats were randomly divided into five groups, namely the normal control group (A ) , model group (B ) , colchicine group (C group ) , Qingxiangsan high dose group (D ) , middle dose Qingxiangsan group (E ) . In addition to group A , the remaining rats were injected subcutaneously in the experimental section 1d each 40% ( volume fraction ) CCl 4 peanut oil 0.5ml · 100g -1 ; thereafter every three days injection of 0.3ml · 100g -1 . Of each group are 79.5% pure corn flour with 20% lard , and 0.5 % cholesterol diet mixed , and 30% ethanol for a drink . A group injected with equivalent peanut oil , eating normal food and water ad libitum . In each group the first two days of modeling daily gavage corresponding drugs , C group fed daily colchicine 0.011mg · 100g -1 , D, E group were fed daily 2g Qingxiangsan · 100g -1 , 1g · 100g -1 , B group and A group fed with distilled water as a control. The sixth week , the animals were killed , blood and liver tissue aside. Part of the liver tissue fixed in 10 % neutral formalin solution . Normal liver tissue dehydrated , embedded in paraffin , sliced ​​thick 5μm, doing light microscopy , in situ hybridization and immunohistochemistry using ; portion fixed with 2.5 % glutaraldehyde for electron microscopy use . According to kit instructions , respectively, with Lai's method and the colorimetric determination of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity ; digestion assay liver liver hydroxyproline (Hyp), xanthine oxidase measuring liver tissue superoxide dismutase (SOD), thiobarbituric acid (TBA) method to measure liver tissue malondialdehyde (MDA). Serum total protein (TP), albumin (ALB), globulin (GLB) using automatic biochemical

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