Dissertation > Excellent graduate degree dissertation topics show

Experimental Studies on the Construction and Application in Distraction Osteogenesis of the Nano Cellular Injectable Tissue-engineered Bone

Author: WangMingHai
Tutor: ZhangXiQing;WangXiaoDong
School: Suzhou University
Course: Surgery
Keywords: Marrow Bone marrow stromal cells Proliferation Differentiation Osteoblasts Vascular endothelial cells In vitro culture Co-culture Tissue engineering Vascularization Injectable Nano Hydroxyapatite Collagen Biocompatibility Distraction osteogenesis Bone density Biomechanics
CLC: R318.0
Type: PhD thesis
Year: 2005
Downloads: 295
Quote: 0
Read: Download Dissertation


A bone marrow stromal cells induced to differentiate into bone cells and renal vascular endothelial cells directly co-cultured in vitro research purpose of this experimental study designed to cultured bone marrow stromal cells (BMSCs) induced osteoblasts and renal vascular endothelial cells as tissue engineering cells and their biological characteristics , and direct co-culture of both good cell compatibility in order to observe the direct contact of the two types of cells , and to explore the appropriate proportion of the two co-culture . Extracted rabbit bone marrow , bone marrow stromal cells obtained by centrifugation , obtained by the conditioned medium of osteoblasts ; renal microvascular endothelial cells using a three-step gradient mesh kidney glomus explants law ; direct co-culture of the two types of cells in different proportions observe the cell morphology , the count cell proliferation ability detection of alkaline phosphatase (ALP) activity , study co-cultured osteoblasts osteogenic ability to change . Results of BMSCs in the conditions of the culture medium induced to differentiate into osteoblasts , from the growth and proliferation of cells co-cultured cells proliferating faster than a single cell culture was identified alkaline phosphatase and mineralized nodule stained positive ; osteoblasts cultured endothelial cells ratio of 2:1 is better than 3:1 and 1:1 ratio culture , proliferation fastest ; osteoblast ALP staining positive rate , and vascular endothelial cells co-cultured osteoblasts stained positive rate than simple osteoblasts , osteoblasts and endothelial cells cultured ratio of 2:1 is better than 3:1 and 1:1 ratio of culture , the highest positive rate , indicating that vascular endothelial cells can significantly enhance osteoblast ALP activity . Conclusion Bone marrow stromal cells can be induced into osteoblasts in vitro ; of BMSCs induced osteoblast and renal vascular endothelial cells in vitro direct co-culture with good compatibility , can promote the proliferation and differentiation of osteoblasts , and promote the ALP secretion , while increasing the capacity of the osteogenic cells into bone ; the both preferred proportion 2:1 mixture .

Related Dissertations

  1. The Establishment and Preliminary Application of the Three-dimensional Finite Element Model of Ankle Joint,R687.3
  2. The Biomechanics and Imageology Preliminary Studies of Lumbar Intertransverse Absorbable Fusion Cage in the Process of Biodegradation,R687.3
  3. Shandong Province 64kg men's boxers before Zhiquan Biomechanical analysis technique,G886.1
  4. Application Research of the Evaluation of Occlusal Contacts in the Orthognathic Surgery Based on ABAQUS,R782
  5. The Comparative Study of Two Deproteinized Methods on Xenogeneic Bone Graft Substitute Material,R687.3
  6. The Theoretical and Experimental Research of the Absorbable Patella Net,R687.3
  7. A Study on Design and Biomechanics of Enhanced Bioceramic Rod,R687.3
  8. Finite Element Analysis of the Effects of Traction on the Biomechanical Behavior of the Degenerated Cervical Spine,R681.55
  9. Comparison of Effects and Biomechanical Study between DHS and LCP Fixation in Treatment of Intertrochanter Fracture of Femur,R687.3
  10. A New Way Based on Computational Fluid Dynamics Study the Asthma Mechanism by Using a Real Airway Model,R562.25
  11. Lower limb kinematics analysis and modeling,R318.01
  12. Biomechanical Analysis of Nano-hydroxyapatite/Polyamide (n-HA/PA) Composite Cervical Vertebra Scaffolds,R318.01
  13. Rabbit skin inflamed by vaccinia virus extract injection on fracture healing and its molecular mechanism of experimental study,R965
  14. Posterior lumbar different surgical evaluation of the stability of the lumbar spine,R687.3
  15. Acetabular transverse fracture risk zone security different biomechanical fixation methods,R687.3
  16. Tibial angle adjustable self-locking anatomical plate design and biomechanical research,R687.3
  17. The Arc-Track Private Lock Pedicle Orthpedics Fixation System Ⅱ Design Research and Clinical Application,R687.3
  18. Experimental study of posterior cruciate ligament rupture patella biomechanics,R686.5
  19. Biomechanical Research of Monosegment Pedicle Instrumentation in Spinal Tuberculosis,R687.3
  20. Heterogeneous experimental study of biological bone screw,R687.3
  21. Biomechanical Study on the Stress Distribution in the Hip Dome Region to Knee Joint in Different Degrees of Varus and Valgus,R687.3

CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > General issues
© 2012 www.DissertationTopic.Net  Mobile