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Experimental Studies on the Construction and Application in Distraction Osteogenesis of the Nano Cellular Injectable Tissue-engineered Bone

Author: WangMingHai
Tutor: ZhangXiQing;WangXiaoDong
School: Suzhou University
Course: Surgery
Keywords: Marrow Bone marrow stromal cells Proliferation Differentiation Osteoblasts Vascular endothelial cells In vitro culture Co-culture Tissue engineering Vascularization Injectable Nano Hydroxyapatite Collagen Biocompatibility Distraction osteogenesis Bone density Biomechanics
CLC: R318.0
Type: PhD thesis
Year: 2005
Downloads: 295
Quote: 0
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Abstract


A bone marrow stromal cells induced to differentiate into bone cells and renal vascular endothelial cells directly co-cultured in vitro research purpose of this experimental study designed to cultured bone marrow stromal cells (BMSCs) induced osteoblasts and renal vascular endothelial cells as tissue engineering cells and their biological characteristics , and direct co-culture of both good cell compatibility in order to observe the direct contact of the two types of cells , and to explore the appropriate proportion of the two co-culture . Extracted rabbit bone marrow , bone marrow stromal cells obtained by centrifugation , obtained by the conditioned medium of osteoblasts ; renal microvascular endothelial cells using a three-step gradient mesh kidney glomus explants law ; direct co-culture of the two types of cells in different proportions observe the cell morphology , the count cell proliferation ability detection of alkaline phosphatase (ALP) activity , study co-cultured osteoblasts osteogenic ability to change . Results of BMSCs in the conditions of the culture medium induced to differentiate into osteoblasts , from the growth and proliferation of cells co-cultured cells proliferating faster than a single cell culture was identified alkaline phosphatase and mineralized nodule stained positive ; osteoblasts cultured endothelial cells ratio of 2:1 is better than 3:1 and 1:1 ratio culture , proliferation fastest ; osteoblast ALP staining positive rate , and vascular endothelial cells co-cultured osteoblasts stained positive rate than simple osteoblasts , osteoblasts and endothelial cells cultured ratio of 2:1 is better than 3:1 and 1:1 ratio of culture , the highest positive rate , indicating that vascular endothelial cells can significantly enhance osteoblast ALP activity . Conclusion Bone marrow stromal cells can be induced into osteoblasts in vitro ; of BMSCs induced osteoblast and renal vascular endothelial cells in vitro direct co-culture with good compatibility , can promote the proliferation and differentiation of osteoblasts , and promote the ALP secretion , while increasing the capacity of the osteogenic cells into bone ; the both preferred proportion 2:1 mixture .

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