Dissertation > Excellent graduate degree dissertation topics show

1.Construction of Fusion Gene Coding for RGD Peptide and Urokinase B Chain and Its Expression in Escherichia Coli and Characterization 2.The Study on Suppression of Metastatic Potential of Cancer Cells with Urokinase Amino-terminal Fragment Gene Tran

Author: ZhuFuXiang
Tutor: HeFuChu
School: PLA Military Academy of Medical Sciences
Course: Cell Biology
Keywords: RGD peptide urokinase B-chain fusion gene fibrinolysis anti-platelets Urokinase Amino-terminal fragment Gene transfer Metastasis
CLC: R346
Type: PhD thesis
Year: 1999
Downloads: 152
Quote: 0
Read: Download Dissertation


一 Construction of Fusion Gene Coding for RGD Peptide and Urokinase B Chain and Its Expression in Escherichia coli and CharacterizationA RGD peptide-coding cDNA was chemically synthesized and fused with urokinase B-chain cDNA and then cloned into the prokaryotic expression vector pBV220. The fused gene was expressed in E. coli DH5α under the control of PRPL promoter by 42 ℃ heat induction. The expression level of the fused gene was over 9.2% of the total bacterial proteins as an inactive form of inclusion bodies. The isolated products were obtained by denaturation and renaturation. It was demonstrated that the fusion proteins have the similar antigenicity, fibrinolysis as entire urokinase and displayed anti-platelets aggregation activity in vitro. 二 The Study on Suppression of Metastatic Potential of Cancer Cells with Urokinase Amino-terminal Fragment Gene TransferTo explore the suppressive effects of gene transfer of urokinase amino-terminal fragment (ATF) on metastatic potential of cancer cell, the eukaryotic expression vector containing ATF cDNA controlled under CMV promotor/enhancer was constructed and transfected into a highly metastatic human lung cancer PG and breast cancer MCF-7 cells line, which expressed uPA and uPAR. Then the expression of ATF was analyzed by means of reverse transcription polymerase chain reaction (RT-PCR). Latter, we observed the effects of ATF gene transfer on invasive potential of cancer cells in vitro. To determine the effcts of ATF gene transfer on tumorigenesis and spontaneous metastasis, the gene-transfected cancer cells were inoculated subcutaneously into nude mice. After ATF gene transfection, the ATF mRNA was obviously expressed. The invasive potential through matrigel in vitro of these cancer cells was decreased significantly. Its tumorigenesis was not affected but its spontaneous metastasis potential remarkably inhibited. Therefore, ATF gene transfer results in the suppression of spontaneous metastasis through competitive binding of exogenous ATF to urokinase receptor with urokinase.

Related Dissertations

  1. The Regulate and Controlof Slug on P53-MDM2 System,R730.2
  2. Analyzing Drought Tolerant Related Characteristics of Transgenic Wheat Lines with the TPSP Gene,S512.1
  3. Characterization of a Mp-Degrading Strain Sphingopyxis sp.DLP-2, Cloning and Functional Verification of Tnmpd,X172
  4. The Contribution of the CCl22, CCR4 in the Milky Spots Peritoneal -metastasis of Gastric Cancer,R735.2
  5. Clinical Analysis of Postoperative Recurrent-metastatic Gastrointestinal Stromal Tumors,R735
  6. The Effects of Reserving Time of Filling Urokinase on Stereotactic Hematoma Aspiration of the Rabbit Intracerebral Hemotoma and the Heme Oxygenase -1 (HO-1) Expression,R743.3
  7. The Study on Expression of CLIC1 on Lymphatic Metastasis of Mouse Hepatocarcinoma,R735.7
  8. The Location and Expression in Mouse Heptocarcinoma Cell Lines with High and Low Lymphatic Metastatic Potentials.,R735.7
  9. The Study of the Correlation of Prognosis and Pattern Evaluation of Patients with Recurrence of Rectum Cancer,R735.37
  10. Restructuring lampreys RGD peptides antithrombotic Pharmacodynamics,R285.5
  11. α1, 3 fucosyl transferase Ⅳ (Fut4) upregulated increased metastatic potential of squamous cell scc12,R730.2
  12. CXCR1/CXR2 receptor antagonists on mouse ascites hepatoma lymphatic metastatic potential impact,R735.7
  13. The Expression of TAFI in the Patient with Polycystic Ovary Syndrome,R711.75
  14. Construction of a Eukaryotic Expression Vector Containing CD59-linker-CD2 Fusion Gene and Its Role in Jurkat Cell Signal Transduction,R392.1
  15. The Effect of Different Concentration of Urokinase on Stereotactic Aspiration of the Rabbit Intracerebral Hematoma and the Expression of the Heme Oxygenase-1,R743.34
  16. The Effects of Down-Regulating MSI2 Gene on BCR-ABL Fusion Gene Expression in K562 Cell Line,R55
  17. The Role and Significance of the Expression of TGFA in Breast Cancer Bone Metastasis Cells,R737.9
  18. The Effect of MACC1 on Invasion and Metastasis in Prostate Cancer Cell Lines (1Ee8/2B4),R737.25
  19. Inhibition of CD24 Expression to Induce Anoikis in Ovarian Cancer Cells,R737.31
  20. The Expression of Hexokinase Ⅱ in Nasopharyngeal Carcinoma,R739.63
  21. The Synergistic Role of Inactivated Smad4 and Activated Kras G12D in Liver Metastasis of Colon Cancer,R735.35

CLC: > Medicine, health > Basic Medical > Human biochemistry, molecular biology
© 2012 www.DissertationTopic.Net  Mobile