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Research on Measurement of Persist Mycobacterium Tuberculosis and Value in Clinic

Author: GaoMengQiu
Tutor: ZhuLiZhen
School: Beijing Tuberculosis and Thoracic Tumor Research Institute
Course: Internal Medicine
Keywords: Mycobacterium tuberculosis persister mRNA (RT-PCR)Reverse transcription-polymerase chain reaction (ICL)isocitrate lyase Acr (alpha-crystallin chaperone ) 85B protein
CLC: R450
Type: PhD thesis
Year: 2006
Downloads: 230
Quote: 0
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Abstract


Background:Tuberculosis is an important infectious disease. Although chemotherapy can cure 95% of the new tuberculosis patient, but the long term of the disease, and the relapse are the main barrier of the control of the disease. The persist tuberculosis are believed as the causes. At the microaerophilic or anaerobic conditions, the metabolism of mycobacterium tuberculosis shiftdown to glyoxylate bypass circumvents, and the express of isocitrate lyase and the 16-kilodalton α -crystallin chaperone protein hemologue (Acr) are expressed highly and cell wall thickening in shape. The persister are usually resistant to chemotherapy and it can be able to avoid elimination from the human host by immune system. Objective:At first, to set up a persistence model in vitro and in vivo and observe the metabolic state of TB in different course of chemotherapy by detecting of mRNA. Methods:(1) Compared the levels of mRNA of ICL、 Acr and 85B in different conditions in vitro, and the levels of mRNA were determined by quantitative RT-PCR.(2) Cultured tuberculosis detected the expression of mRNA of the ICL、 Acr and 85 B in Cornell model in different period.(3) Detected the expression of mRNA of ICL、 Acr and 85B in operation materials from 12 patients who had received chemotherapy at least for 3 month.Results:Both ICL and Acr are expressed highly in vitro and vivo models, the ratio of ICL/85Bare concerned with the metabolist state of MTB and we also detected the highly

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