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The Study on Biosynthetic Mechanism of Juvenile Hormone in Apriona Germari and Its Regulation on Reproduction

Author: TianLing
Tutor: ZuoBaoZhong
School: Nanjing Forestry University
Course: Forest Protection
Keywords: Apriona germari Juvenile hormone Biosynthetic mechanism Reproductive regulation
CLC: S763.38
Type: PhD thesis
Year: 2008
Downloads: 180
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Abstract


In this thesis,A. germari was chosen as the study object for researching JH biosynthetic mechanism.The biosynthetic ability of JH in its endocrine and reproductive organs were studied.The effects of JH on reproduction were also researched. The main results as follows:1.The reproductive systems of male and female adults were both described for the first time. The contents of the total carbohydrate and protein in the hemolymph, male reproductive systems, MAG,ovaries from both virgin and mated males and females were tested.The pH in above tissue and organs were also detected.2. Results showed that CA couldn’t synthesize JH independently,the testis, MAG and ovary of A. germari were found as organs finally to synthesize JHⅢ. By the use of radiochemical assay,the biosynthetic rate of JH in MAG and testis in vitro were studied.The highest biosynthetic rate of JH in MAG and testis was respectively at 6h and 8h.3. By the use of HPLC,we found that the biosynthetic pathway of JHⅢin A. germari adults was from FA to MF and then MF was converted to form JHⅢ. The CA of both sexes could use farnesol to synthesize FA,FA couldn’t be converted to form MF and JHⅢin CA.Testis,MAG and ovary could use MF to synthesize JHⅢby adding MF.The biosynthetic product of CA was FA.It was methylated to form MF by methyltransferase coming from haemolymph when there is methyl donor.MF finally completed the epoxidation to form JHⅢin above reproductive organs.4.After incubated for 30h in dark under 28℃,the embryo begun to synthesize JH with 3H-Met.The biosynthetic pathway of JHⅢwas from FA to MF and then to JH.It was inferred that FA and MF were the main form of JHs in embryo.The respective detection of neonatal larvae and oothecae showed that JHⅢonly existed in larvae,but there were large amount of FA and MF on oothecae.It meaned that the JHs in neonatal larvae originated from female and synthesized by itself.5.Medium 199 containing 3H-Met was injected into male adults.After raised for 24h,some male were chosen to mate with female,the radioactivity in haemolymph,reproductive system of mated female were detected.Results showed that there were large amount of radioactivity in male haemolymph and reproductive system before and after mating.The radioactivity in MAG obviously decreased after mating.By the use of HPLC,we found that the content of JHⅢin mated MAG decreased for 41.63%,but it almostly didn’t change in haemolymph.The content of JHⅢin mated female haemolymph and ovary both increased.6. The phosphate buffers(pH 7.0) under 18℃and 4℃which contained hemolymph were chosen as culture condition. Effects of JHⅢand MAG secretions on the uptake of vitellogenin were tested.Results showed that both of JHⅢand MAG secretions could stimulated the uptake of Vg.The best dissolved liquid system was choseMeanwhile, the JH and MAG secretions was injected into female,both of them can increased the feeding amount,female receptivity, fecundity in vivo.

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CLC: > Agricultural Sciences > Forestry > Forest Protection > Forest Pest and Disease Control > Pest and its control > Coleoptera
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