Dissertation > Excellent graduate degree dissertation topics show

Theoretical and experimental study of TCM Yin Deficiency

Author: RenXiaoJuan
Tutor: WangZuo
School: Beijing University of Traditional Chinese Medicine
Course: Basic Theory of Traditional Chinese Medicine
Keywords: yin deficiency constitution theoretical research gene expression profile endocrine function immune function
CLC: R228
Type: PhD thesis
Year: 2008
Downloads: 662
Quote: 4
Read: Download Dissertation

Abstract


Objective To construct the theoretical basis of yin deficiency constitution. To study on peripheral blood gene expression profile in the yin deficiency constitution by gene chip technique. To observe the changes of endocrine function and immune function in subjects with yin deficiency constitution and explore the relationship between the biochemical indexes and the yin deficiency constitution.Methods By the methods of the traditional literature research and the contemporary literature analysis,we investigate the cognition of yin deficiency constitution in the last time,the presentation and determination,the formative factor,the regulating method,the health cultivation of yin deficiency constitution,and the difference and relation of yin deficiency constitution and yin deficiency syndrome.Eight cases of yin deficiency constitution and six cases of normal constitution were selected according to determinant criterion of yin deficiency constitution and normal constitution. Ten milliliters of fasting venous blood were collected from each subject. The white blood cells were isolated,then the total RNA was extracted,purified and amplified. The complementary RNA was labeled with biotin. The gene expression profile of peripheral blood was detected with Affymetrix Gene Chip Human Genome U133+2.0 and the biological functions of differentially expressed genes were evaluated.Sixty subjects with yin deficiency constitution and fifty ones with normal constitution were selected according to determinant criterion of yin deficiency constitution and normal constitution on the basis of epidemiology investigation. Eight milliliters of fasting venous blood were collected from each subject at eight o’clock to nine o’clock. The sera were separated by five minutes of centrifugation at 3000 r/min,and they were stored at temperature of -70℃.The serum levels of corticosterone,cortisol,adrenocorticotrophic hormone,cyclic adenosine monophosphate,cyclic guanosine monophosphate,free triiodothyronine,free thyroxine,thyrotropic-stimulating hormone,interleukin-1βand interleukin-2 were assayed using enzyme linked immunosorbent assay,the cAMP/cGMP ratio was computed,and the difference of above-mentioned indexes was statistically analyzed between yin deficiency constitution and normal constitution.Results Yin deficiency constitution is one of the common types of constitution which is affected by congenital heredity,acquired predisposition,social factor,etc. Fever and drying are its principal characters.1142 probe sets of differentially expressed genes were identified between the yin deficiency constitution and the normal constitution. Of the 1142 probe sets,645 were up-regulated and 497 down-regulated. As q<0.05,there were 116 biological functions in GO annotation,22 pathways in KEGG analysis,87 pathways in GenMAPP analysis and 30 pathways in bioCartr analysis.The serum levels of free triiodothyronine of yin deficiency constitution significantly increased compared with those of normal constitution,and the serum levels of cortisol,adrenocorticotrophic hormone,cyclic guanosine monophosphate and free thyroxine of yin deficiency constitution significantly decreased in comparison with those of normal constitution. The serum levels of cortisol of yin deficiency constitution significantly increased compared with those of yang deficiency constitution,and the serum levels of corticosterone and cyclic adenosine monophosphate of yin deficiency constitution significantly decreased in comparison with those of yang deficiency constitution. Conclusions Complementing yin and clearing away heat are the main methods for regulating yin deficiency constitution. Invigorating the spleen and promoting the flow of qi at the same time.There were significant differences of peripheral blood gene expression profile between the yin deficiency constitution and the normal constitution. Inflammatory reaction of yin deficiency constitution is severer than normal constitution. The biosynthesis and metabolism function strengthen,but the enzymatic activity,the gene expression process and the growth and development have downtrend.Yin deficiency constitution might be related with the functional disorder of hypothalamic-pituitary-adrenal axis and hypothalamic-pituitary-thyroid axis as well as the functional disorder of cyclic nucleotide system and immune system.

Related Dissertations

  1. Effects of Plant Essential Oil on Growth Performance , Intestinal Microflora and Immunization Performance in Piglets,S828.5
  2. Identification and Analysis of miRNA and Flower Development Specific Genes in Wheat,S512.1
  3. The Study on the Effect of the Transport Stress and Anti-stress Treatment on the Physiological Function of Beef Cattle,S858.23
  4. Effects and Some Mechanisms of TACI-Ig on Immune Function in MRL/lpr Mice,R593.241
  5. Study on Enhancing Immune and Toxicology of Propolis Soft Capsule,R285
  6. The Regulatory Role of Proliferation and Apoptosis of ERK1/2 and JNK Signaling Pathways in Eight Liver Cell Types during Rat Liver Regeneration,Q418
  7. Psoriasis vulgaris in different stages and card Hou T lymphocyte subsets,R275.9
  8. Effects of Postoperative Analgesia with Parecoxib Sodium and Fentanyl on the Immunity of Gynecological Patients,R614
  9. Effects of Different Antioxidants on Antioxidant Ability, Immune Functions and Related Gene Expressions in Yellow-Feathered Broilers,S831.5
  10. The Influence of Omega 3 Polyunsaturated Fatty Acid on the Immune Function of Sepsis,R459.7
  11. Research on the Effects of Different Routine Experimental Procedures and Rearing Environment on Wistar Rats,S865.12
  12. Effects of Achyranthis Bidentatae Polysaccharides (ABPS) on Growth Performance, Immune Function and Intestinal Function in Piglets Challenged with Lipopolysaccharide,S828.5
  13. A Clinical Study on Jiangqi Hewei Tongfu Method to Promote Gastrointestinal and Immune Function for Postoperative patients with Intestinal Tumor,R273
  14. Chinese medicine decoction of the cellular immune function of patients with malignant tumors,R273
  15. Theoretical and Clinical Study on Treatment of Diabetic Retinopathy Based on the Collateral Theory,R259
  16. Theoretical Research on Photochemical Technique of Laser Isotope Separation,TL922
  17. Effect of Radix Hedysari and Its Compound on Performance, Antioxidation and Immune Function of Broilers,S831.5
  18. Study on Effects of Probiotics on Production Performance and Immune Function of Weaning Piglets,S828.5
  19. The Effect of Bacillus Subtilis Preparation on Growth Performance in Weanling Piglets,S828.5
  20. Experimental Study of Cell Immune and Intestinal Barrier After Severe Trauma - Hemorrhagic Shock in Rats,R641
  21. Integrative Medicine Clinical study of chronic pyelonephritis,R692.7

CLC: > Medicine, health > Chinese Medicine > Basic Theory of Traditional Chinese Medicine > Chinese medicine pathological
© 2012 www.DissertationTopic.Net  Mobile