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Experimental Study on Electro-Nape-Acupuncture Protection Mechanisms of Cerebral Ischemia-Reperfusion Injury Rat

Author: ZhuXiaoJu
Tutor: GaoWeiBin
School: Heilongjiang University of Traditional Chinese Medicine
Course: Acupuncture and Massage
Keywords: electro-nape acupuncture Cerebral Ischemia / reperfusion Apoptosis ICAM-1
CLC: R245
Type: PhD thesis
Year: 2008
Downloads: 190
Quote: 1
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Abstract


As a familiar disease, Apoplexy has taken place frequently and the ratio of death and lameness are both very high. Moreover, the cerebral ischemia accounts for 75 percent of Apoplexy, it also has a tendency for increasing year after year . With the application of thrombolytic therapy , the reperfusion injury is paid to more and more attention . Studies showed that the cerebral ischemiareperfusion injury was closely associated with neuronal cell apoptosis, inflammatory reaction, overload of calcium , production of oxygen free radicals and toxicity of excitatory amino acid . So it is an urgent problem for us to prevent and cure the cerebral ischemia or to decrease the ratio of incident and lameness. This topic studies the cerebral protection of electro-nape acupuncture to ischemical reperfusion injury and Potion as well as its mechanism action.Objective : To observe the expression of 1CAM-1 and that of the correlative gene of apoptosis in ischemic penumbra in rats after acute cerebral infarction and to explore the effect and the possible mechanism of electro-nape acupuncture therapy.Methods : The model of focal cerebral ischemia was made by blocking the middle cerebral artery with monofilament in rats. The middle cerebral artery ocelussion rats were randomly divided into 4 groups: sham group (group A), model group (group B), electro-acupuncture group(group C) and electro-nape acupuncture group (group D). Male Wistar rats were divided randomly into four groups:sham group, model group, electro-nape acupuncture group and electro-acupuncture group group;each group was separated into 12h, 1d, 3d, 5d after rerperfusion as subgroup for investigation. HE staining and TUNEL were used to observe the changes of histomorphology. Took Immunohisto chemistry and In situ hybridization to detect the expression of Bcl-2、Fas-1( mRNA)、ICAM-1( mRNA), and also observe ultrastructure in brain tissue、nerve function Score.Results: Electro-nape acupuncture has the obvious auxoaction on recuperation of neurologic impairment. The results showed electro-nape acupuncture possessed protective effect against cerebral ischemia -reperfusion injury. compared with the model group. Electron microscope results indicated that compared with group B, the neuron ultrastructure was mended in group C and in group D.It was more dominant in group D than that in group C. Electro-nape acupuncture can lessen neurologic impairment. Results pointed out, that compared with the model group, electro-acupuncture group and electro-nape acupuncture can increase the expression of Bcl-2 and restrain the expression of Fas-1(mRNA), ICAM-1(mRNA).Conclusion: Electro-acupuncture group and electro-nape acupuncture can save the neurocytes in peri-infarct region and reinforce the blood supply so as to amend neural function. Electro-nape acupuncture can lessen neurologic impairment, repress apoptosis, promote Bcl-2 expression,heighten Fas-l(mRNA)expression and inhibit ICAM-1(mRNA) expression, which indicates that the action on repressing apoptosis is related to the regulation on Bcl-2, Fas-1(mRNA), ICAM-1(mRNA) expression.

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