Dissertation > Excellent graduate degree dissertation topics show

Antiviral Effect of PSM Against Herpes Simplex Virus Type2

Author: ChenZuo
Tutor: WuZhiWei; LiuJianNing
School: Nanjing University
Course: Immunology
Keywords: 4-styrene sulfonic acid copolymer of maleic anhydride(PSM) herpes simplex virus type2(HSV-2) HSV-2vaginal infectionmodel in mice antiviral
CLC: R373
Type: Master's thesis
Year: 2012
Downloads: 13
Quote: 0
Read: Download Dissertation


Herpes simplex type Ⅱ (HSV-2), a double stranded linear DNA virus, is a member of the herpesviridae family. HSV-2is transmitted mainly through sexual route and is one of the most prevalent sexually transmitted diseases globally. Most of the infection is not fatal and interspersed with asymptomatic latency and clinically active viral replication. HSV-2infection results in genital microleisions and mucosal inflammation and is demonstrated epidemiologically to be a risk factor for HIV-1transmission. A meta-analysis also demonstrated that HSV-2infection also influences the disease progression in the HIV-1infection population. The existing treatment with acyclovir when applied early in the infection will only reduce the frequency of recurring viral replication and lessen severity of the disease but will not cure or clear the virus. From the point view of public health, the most cost effective and efficient approach is to prevent HSV-2transmission at the entry portal. However, current vaccine is of limited efficacy and an effective entry inhibitory drug is still elusive. Therefore, effective drugs are critically needed. To explore how Poly (4-styrenesulfonic acid-co-maleic acid)(PSM) blocks HSV-2infection, in vitro and in vivo researches were carried out as following:1.After the pGL4plasmid containing the recombinant ICP10-promoter was constructed, Vero cells were treated with different concentrations of PSM (60.20、6.67、2.2、0.74、0.25、0.08ug/mL), followed by50uL HSV-2(MOI=1). Luciferase analysis of ICP10-promoter was performed to measure the virus replication in vitro. The results showed that PSM could inhibit HSV-2infection of the Vero cells.2.gD protein was analyzed in an In-Cell-Western assay to show the inhibitory effects of PSM on HSV-2infection of HEC-1-A cells. The results showed that PSM could inhibit HSV-2infection of HEC-1-A cells.3.A mouse model was developed to investigate the in vivo efficacy of PSM in protecting the animals from HSV-2infection via vaginal route. The results showed that PSM offered significant protection of the mice against the vaginal infection of a pathogenic HSV-2strain (P<0.001).The above experimental results show that PSM could be a potential candidate for the treatment against HSV-2, and further research is needed to expand the scope of the investigation and for a thorough evaluation of toxicity.

Related Dissertations

  1. Improvement of the Resistance of Nicotiana Tabaccum to Virus by RNAi and Initial Establishment of Genetic Transformation System of L.regale,S435.72
  2. Genotypic HIV-1 Drug Resistance and Factors Accociated with the Effect of Antiretroviral Therapy among Chinese HIV/AIDS Patients,R450
  3. The Direct Medical Costs Analysis of National Free Antiretroviral Treatment for HIV/AIDS Patients,R512.91
  4. Asparagus polysaccharide sulfate group content and anti-influenza virus activity relationship studies,R285
  5. Isolation and Function Identification of a Novel RNA-dependent RNA Polymerase Gene, GhRdRP, in Cotton (Gossypium Hirsutum L.),S562
  6. Studies on Isolation, Purification and Antibacterial and Antivirus Activity of Ostrich Neutrophil Antimicrobial Peptides,S852.4
  7. The Research of Novel Acyclic Nucleoside Antiviral Drugs,R978.7
  8. Evaluation of the Antibacterial and Antitumor Activity of Tibetan Medicinal A. Tanguticum and Anti-HSV-2 Activity of Tibetan Medicinal L. Brevituba,R291.4
  9. Primary Screen of Active Sites Extracted from Actinidia Chinensis,R284
  10. Toxic hot flat injection on FM_1 mice infected macrophage function in rats,R285.5
  11. The Roles of Micro-RNAs in the Effector Function of Type Ⅰ Interferon and the Underlying Mechanisms,R392
  12. Human Leukocyte Antigen(HLA)-DRB1 Polymorphism is Associated with Familial Hepatitis B in Shanxi,R512.62
  13. Anti- virus-related pharmacophore Construction and Evaluation Methods,R91
  14. Study on the Anti-HSV Activity of Polyphenols from Phyllanthus Emblica L. and Investigation of Its Possible Mechanisms,R285
  15. Prokarotic Expression and Purification of Porcine Interferon-α Interferon-γ,S858.28
  16. Detoxification capsules pharmaceutical research,R285.5
  17. A hospital in Changsha, HIV / AIDS and hepatitis B antiviral treatment adherence research,R512.62
  18. Studies on Extracting Technology of TFG and Biological Activity of Geranium Wilfordii Maxim.,S567.239
  19. Establishment of the Serum Specimen Repository of HCV Infected Patients and Effect Observation of Antiviral Therapy to Hepatitis C Patients with Liver Cirrhosis after Splenectomy or Partial Splenic Embolization,R575.2
  20. Prokaryotic Expression and Actvity Assay of Recombinant Porcine Interferon-alpha,Q78
  21. Screening of the Antagonistic Bacteria Against CMV and Study on Its Mechanism,S476.1

CLC: > Medicine, health > Basic Medical > Medical Microbiology ( pathogenic bacteriology,pathogenic microbiology ) > Human Virology ( pathogenic virus)
© 2012 www.DissertationTopic.Net  Mobile