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Virus-induced Gene Silencing as a Tool for the Founctional of Several Floral Homeotic Genes in Pisum Sativum

Author: JiaFeiFei
Tutor: WangLiLin
School: Hangzhou Normal University
Course: Genetics
Keywords: Peas Flower homeotic transformation Pim PsSOCla PsSVP
CLC: Q943.2
Type: Master's thesis
Year: 2011
Downloads: 43
Quote: 0
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Abstract


VIGS system based on the pea early as brown Virus (pea early browning virus, PEBV) pea flowers homologous ABCE class represents profiled transition of gene PEAM4 PsPI PsAG and PsSEP1 / 2 5 'end of the corresponding sequence encoding MADS domain VIGS vector construction and virus inoculation , phenotype observed inoculated plants and do genetic statistical analysis , results show that the highly conserved segment Select VIGS can be a highly conserved homologous gene silencing out . In addition to the pea APETALA1/FRUITFULL-Like homologous gene PEAM4 conservative and specific sections PsFUL conservative district VIGS construct and virus inoculation . PsFUL conservative area of VIGS results show that the change in the pattern on the obvious . Meanwhile, for PEAM4 , respectively their conservative segments and specific segments VIGS construct , the results showed that these two constructions were well simulate the phenotype of the mutant PIM , which has two mutant forms , respectively, pim-1 and pim-2, specific performance has a great influence to delay the differentiation of the floral meristem and flower morphology , which is similar to the Arabidopsis APETALA1 (AP1) mutant body apl phenotype . In wild-type Arabidopsis AP1 by inhibiting a group of flowering time genes AGL24, SVP, SOC1 to maintain the attributes of the floral meristem prevent reversal of flowers . These genes ectopic expression in the absence of AP1 . Phylogenetic tree analysis , to find the pea in the SVP SOC1 the homologous genes PsSOC1a and the PSSVP , and the use of quantitative PCR and in situ hybridization to detect the expression patterns they PEAM4 silence strains variety a PEAM4 of VIGS the phenotype is caused by the PsSOC1A and PsSVP ectopic expression .

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CLC: > Biological Sciences > Botany > Plant Cell Genetics > Plant Genetic Engineering
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