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Effects of Different Dosages X-ray on Antioxidant Enzymes Activity, Histological Structure and Expression of Inter Related Polypeptides in the Lungs of the Filial Mice

Author: GuoZuoZuo
Tutor: YuShiYuan
School: Northwest Normal University
Course: Cell Biology
Keywords: X-ray Pups Antioxidant enzyme activity Organizational structure Bax KGF
CLC: Q691
Type: Master's thesis
Year: 2010
Downloads: 20
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Abstract

Objective: through different doses of X-ray radiation, the growth and development of the study of the period, Aberdeen mouse lung tissue antioxidant enzyme activities, organizational structure and related specific protein expression of dynamic changes, investigate the combined effects of X-rays on the growth and development of animal lung tissue to provide experimental evidence for developmental biology research and clinical ionizing radiation disease treatment and space radiation protection. Methods: 5-7 day-old Kunming mice were randomly divided into six groups, each group of 32. Were treated daily absorbed dose of X-ray 0.0,0.5,2.0,3.5,5.0,6.5 Gy whole body radiation, three-day continuous radiation. 1,5,10,20 d after radiation were decapitated eight pups, remove the lung tissue, grinding diluted after centrifugation, whichever the supernatant detect SOD, GSH-PX activity and MDA, GSH content changes. Another pups lung tissue using histological methods in the dynamic changes of the organizational structure, and measuring the dynamic changes of the alveolar diameter and thickness of the alveolar septa; Immunohistochemistry was used to observe the lung tissue of Bax the KGF protein dynamic change and image analysis software and stereological measurement method for quantitative analysis of Bax, KGF protein expression intensity. Results: 1.X-ray radiation affect the growth and development period Tsai rat lung tissue SOD activity. By the 0.5Gy and 2.0Gy X-ray-irradiated lung tissue SOD activity pups were higher, the difference was significant or extremely significant (P lt; 0.05 or P lt; 0.01). The early increased gradually late lowered and near-normal levels. Offspring lung tissue SOD activity decreased after 3.5Gy, 5.0Gy, and 6.5Gy X-ray radiation, elevated, and were lower than the control group, the difference was significant or very significant (P lt; 0.05 or P lt; 0.01). 2.X-ray radiation affect the growth and development of offspring lung tissue MDA content. By the 0.5Gy and 2.0Gy X-ray-irradiated offspring lung tissue MDA levels were lower than the control group, the difference was significant or very significant (P lt; 0.05 or P lt; 0.01). Gradually decreased early, late increases and near-normal levels. Pups lung tissue MDA content increased at first reduce by 3.5Gy, 5.0Gy, and 6.5Gy X-ray radiation, and were higher, the difference is significant or very significant (P lt; 0.05 or P lt; 0.01). 3.X-ray radiation affect the growth and development of offspring lung tissue GSH-Px activity. After 0.5Gy X-ray radiation pups lung tissue GSH-Px activity were higher, the difference was significant or very significant (P lt; 0.05 or P lt; 0.01), and over time showed a downward trend. GSH-PX activity the pups in lung tissue after 2.0Gy, 3.5Gy, 5.0Gy and 6.5Gy X-ray radiation are lower than those in the control group, the difference was significant or very significant (P lt; 0.05 or P lt; 0.01), and showed first decreased and then gradual recovery trend. 4.X-ray radiation affect the growth and development of the offspring in the lungs GSH content. After the initial period after 0.5Gy X-ray radiation, the pups lung tissue GSH content were higher, a significant difference (P lt; 0.01); the late offspring lung tissue GSH content decreased and close to the control group, the difference was not significant or significant (P gt; 0.05 or P lt; 0.05). GSH decreased lung tissue after 2.0Gy, 3.5Gy, 5.0Gy and 6.5Gy X-ray radiation pups but were lower than the control group, the difference was significant or very significant (P lt; 0.05 or P lt; 0.01). 5.X-ray radiation affect the growth and development period pups lung tissue structure. By different doses of X-ray radiation pups alveolar diameter and thickness of the alveolar septa varying degrees of change. After 0.5 Gy, 2.0Gy X-ray radiation pups pulmonary interstitial cells of dense, compared to the the thickness control group of alveolar diameter and alveolar septa have different degrees of reduction. 3.5Gy, 5.0Gy and 6.5Gy X-ray radiation alveolar diameter and thickness of the alveolar septa and the control group compared to the increase in varying degrees. 6.X-ray radiation affect the growth and development period Tsai Bax protein expression in the lung tissue of mice. Expression of Bax protein in varying degrees in different doses of radiation pups lung tissue, and its expression intensity is enhanced with increasing radiation dose over time gradually returned to near normal levels. 7.X-ray radiation affect the growth and development of the KGF protein expression in the lung tissue of offspring. The KGF protein have different levels of expression of various doses of radiation pups lung tissue. Expression intensity is enhanced with increasing radiation dose, decreased with time and eventually to near normal levels. Conclusion: 1.X-ray radiation on the antioxidant enzyme activity of the growth and development of offspring. Late reduce elevated pups lung tissue superoxide dismutase activity early after small doses of X-ray radiation, MDA content in the initial lower late rise after large doses of X-ray radiation is just the opposite. After small dose X-ray radiation early glutathione peroxidase activity and reduced glutathione content were higher, while the latter were lower than the control group, and are declining over time. 2.X-ray radiation affect the growth and development period pups lung tissue structure. After different doses of X-ray radiation pups alveolar diameter and thickness of the alveolar septa varying degrees of change. Small doses of X-ray radiation pups pulmonary the the dense interstitial cells, alveolar diameter and alveolar septal thickness compared with the control group had varying degrees of reduction. Alveolar diameter and alveolar septal thickness control group compared to the high-dose X-ray radiation increased in varying degrees. 3.X-ray radiation affect the growth and development of offspring lung tissue protein expression of Bax and KGF. Bax and KGF protein in the lung tissue of pups have different levels of expression, the expression of strength is enhanced with increasing radiation dose, and also enhance the induction of cell injury and repair. Specific performance: radiation early in lung tissue cells have a strong positive distribution, followed by Bax protein expression decreased gradually, eventually restored to near normal levels; KGF protein expression showed a downward trend and, ultimately, close to the normal level. High doses of ionizing radiation can cause the body to produce large amounts of superoxide anion radicals (O2-.), resulting peroxide damage cells in the body tissues, and low doses of ionizing radiation can be induced by the organism's immune excited. Ionizing radiation can also be activated so that the function of the body's immune cells, especially T-cell activation and differentiation, aerobic metabolism to produce superoxide anion radical than stationary state. Offspring of lung tissue antioxidant enzyme activity increased after low doses of radiation, lower lipid level. The free radicals produced by large doses of ionizing radiation can not be promptly removed and attack the cell, resulting in tissue damage, antioxidant enzyme activity decreased Bax, KGF protein is highly expressed. Groups pups detection indicators and organizational structure have different degrees of radiation after a period of time to restore the normal trend. That affect the growth and development of X-ray radiation period Tsai mouse lung tissue antioxidant enzyme activities, organizational structure and related specific protein expression. Large doses of ionizing radiation on the growth and development of offspring with damage, but the body has a certain anti-radiation function.

Effects of Different Dosages X-ray on Antioxidant Enzymes Activity, Histological Structure and Expression of Inter Related Polypeptides in the Lungs of the Filial Mice

Abstract

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