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Isolation and Biodiversity Analysis on Bacteria of Magnetite Mine Drainage

Author: AnXiuFang
Tutor: LvZhiTang
School: Hebei University
Course: Microbiology
Keywords: Magnetite Bacterial isolates Diversity Phylogenetic analysis Appraisal
CLC: Q938
Type: Master's thesis
Year: 2011
Downloads: 20
Quote: 0
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Abstract


Hebei the Shahe 2 magnetite pit water sample separation of bacteria, phylogenetic analysis and preliminary identification of bacteria biodiversity in the the magnetite mine water samples analyzed on this basis. The separated results show that the number of isolated colonies of different samples in different media and different types. Best 1/5PCA medium separation effect, neutrophil bacterial magnetite the pit water main bacterial groups, improved 9K improved 9K 'inappropriate separation of magnetite pit water sample bacteria. Preliminary study based on phylogenetic analysis of the taxonomic status of the the magnetite mine water sample isolates genus level and biodiversity. The results show: the the Jiangfeng mining pit water samples 66 bacteria in the phylogenetic part of the 50 OTUs, 36 OTUs the Proteobacteria door (Proteobacteria) the highest sequence similarity (98.4% to 100%); 12 OTUs Actinomyces bacterial phyla (Actinobacteria) the highest sequence similarity (98.9% to 99.9%); the 2 OTUs Bacteroides door (Bacteroidetes) sequence similarity (98.9% to 99.9%). 47 the Qianshan mining pit water samples bacteria under the 32 OTUs in phylogenetic which the the 15 OTUs Proteobacteria door the highest sequence similarity (90.4% to 100%); similar to the bacterial phyla sequence of nine OTUs and actinomycetes the highest (99.4% to 100%); the seven OTUs and Firmicutes (Firmicutes) the highest sequence similarity (93.0% to 100%); 1 the OTU and singular coccus - Thermus door (Denicoccus-Thermus) sequence The highest similarity (100%). Verify the consistency of the phenotypic characteristics and phylogenetic analysis based on the results of a preliminary identification of the morphological characteristics and physiological and biochemical characteristics of the strain, and initially set the level of the taxonomic status of some strains. Strain p1 52 strain was identified as 20 are 38 potential new species; Chinese Shi Nisi coli strain cp15, cp26 and phylogenetic relationships (Sneathiella chinensis) CBMAI 737T highest similarity of 88.2% to 88.3%, Shi Nisi coli Branch (Sneathiellaceae) should be given a new genus status. The biodiversity analysis showed: two samples of bacterial community composition, dominant population is also different genus: the Jiangfeng mining pit water samples in a non-stick handle the majority (19.70%), while the Qianshan mining pit water samples, but shortwave single The dominant cellular genus (25.53%). Analyze the reasons, presumably with the sample source deposit grade and burial depth of the different.

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CLC: > Biological Sciences > Microbiology > Microbial ecology and geographical distribution
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