Dissertation > Excellent graduate degree dissertation topics show

Effect of Flooding Time on Community Structure and Abundance of Geobacteraceae in Paddy Soil

Author: YouJiaoHua
Tutor: WangBaoLi
School: Northwest University of Science and Technology
Course: Biochemistry and Molecular Biology
Keywords: Flooded paddy soil Geobacter Branch Community structure Abundance RFLP
CLC: X172
Type: Master's thesis
Year: 2011
Downloads: 24
Quote: 0
Read: Download Dissertation

Abstract


Geobacter Division bacteria (Geobacteraceae) is a very important alienation Fe (Ⅲ)-reducing bacteria are widely distributed in the reduction of Fe (Ⅲ) environment, such as freshwater sediment, organic matter or heavy metal contaminated groundwater sedimentary environment has important The bioremediation feature. Cyclical changes in the redox state of iron oxide in paddy soil microbial community structure has a significant impact on the flooded paddy soil very important of bacilli bacterial community structure and abundance of dynamic change rarely reported. The test by simulating the paddy soil flooding process, explore the community structure and relative abundance of the the Geobacter Division bacteria (Geobacteraceae) flooding time with dynamic variation, revealing their community structure and relative abundance changes and microbial Fe (Ⅲ) reduction intrinsically linked. Extract Hanzhong (HZ) and Zhejiang (ZJ) paddy soil waterlogged incubation for 1 h, 1 d, 5 d, 10 d, 20 d and 30 d after microbial total DNA of different flooding time samples to build two paddy soils bacilli bacteria 16S rDNA clone library, using PCR-RFLP method Analysis the Geobacter Branch bacterial community structure and diversity in characteristics, dynamic changes by Real-time PCR determination bacilli bacteria relative abundance of some dominant types clones were sequenced and phylogenetic tree was constructed. Using the the anaerobic mud culture method, determination of changes in rice soil Fe (Ⅱ) generation and fitting, and analysis of bacterial community structure and abundance of Geobacter Branch Fe (Ⅲ) to restore the relationship between the Logistic equation. The main results of this test are as follows: (1) HZ paddy soil microbial Fe (Ⅲ) reduction process in flooded early stage of culture change culture reached stationary phase after 20d, the largest iron reduction potential of 10.16 mg / g, maximum reaction rate of 1.064 mg / (gd), the time corresponding to the maximum reaction rate was 4.84 d; ZJ paddy soil flooding after 15 d of culture reached stationary phase Fe (Ⅲ) reduction, the largest iron reduction potential of 8.27 mg / g, maximum reaction The rate is 1.341 mg / (gd), corresponding to the maximum reaction rate of the time was 3.36 d. (2) From the clone library constructed Hz paddy soil samples for 1 h to 30 d, respectively, 64/155, 64/154, 76/162, 62/152, 85/155 and 54/154 RFLP digested type, the capacity value of 61.29% to 81.82%; clone library constructed from 1 h to 30 d ZJ paddy soil samples, respectively, 71/149, 74/139, 75/153, 72/150, 73/151 and 61/153, the storage capacity is 64.75% to 73.86%. (3) α diversity index Hanzhong paddy soil in Geobacter Division bacteria in diversity with waterlogging time fluctuates and changes, inundation 5 d and 20 d treatment 2 peak, and the flooding of 10 d and 30 d treatment significantly reduced the diversity; Zhejiang paddy soil in Geobacter Division bacteria in diversity with flooding extended first increased gradually reduce flooding 1 d highest diversity, diversity is the lowest of the 30 d treatment. Two paddy soils in the β diversity indices showed that the bacilli bacterial community structure in the flooding process there are obvious differences. (4) Different Flooding Hanzhong paddy soil produced a total of 10 farming bacilli bacteria dominant types belong to Clade 1 and Clade 2; Zhejiang paddy soil produced the same 10 farming bacilli bacteria dominant types in Clade 1 Clade 2 and Clade GM, are distributed with participation from freshwater surface sediments and contaminated water, but are far strains distance. (5) Real-time PCR results show that, the Hanzhong paddy soil ground bacilli bacteria and total bacteria 16S rDNA abundance ratio in the waterlogged incubation minimum (1.20%) 1 d, 20 d reached maximum (4.54% The); Zhejiang paddy soil, the relative abundance of the bacilli bacteria in waterlogged incubation 1 d smallest (0.413%), and 10 d reached a maximum (4.111%). Can be drawn through the results, waterlogged anaerobic culture of paddy soil, of Geobacter Branch bacterial diversity and relative abundance over time dynamic changes, the dynamic changes with Fe (Ⅲ) reduction process is closely related.

Related Dissertations

  1. The Changes of Microbial Community Along the Different Trophic Gradient of Seawater in Zhanjiang Bay,Q938.8
  2. Growth Pattern of Marsupenaeus Japonicus and Succesion of Plankton Community in Intensive Model,S968.22
  3. Effects of Different Reconciled Corn Oils on Antioxidative Stress, Lipid Metabolism Enzymes, and Abundance of Immune-related Genes of Broilers,S831.5
  4. Red Soil Microbial Diversity of Peanut Continuous Cropping and Effects of Microorganisms on Growth of Continuous Cropping Peanut,S565.2
  5. Benthic Macroinvertebratre Community Structure and Health Assessement of Li River, Guilin, Guangxi,X826
  6. Investigation of Cereal Cyst Nematode on Wheat in Jiangsu Province and the Sequence Analysis of rDNA-Its Region from Jiangsu Populations,S435.121
  7. Molecular Characterisation Based on ITS and Genetic Diversity of the Heterodera Avenae Group on the Huang-huai Floodplain of China,S435.121
  8. Arthropod Biodiversity in Paddy Fields under Different Management Regimes,S511
  9. Spatial Distribution Characteristics of Community Structure Components in Evergreen Broad-leaved Forest of China,S718.5
  10. The Research of Transgenic Carnation Genetic Stability and the Impact on Soil microbial Community,S682.19
  11. Effect of Different Amendments on Soil Microbial Ecology,S154.3
  12. Jiyuan Oilfield long reservoir evaluation and development technology policy research,P618.13
  13. Functional Genes Diversiyt of Microorganism Related the Cycle of Carbon in Red-Yellow Soil in Southern China,S154.3
  14. The Ecological Research on the Zoobenthos in Ganjiang River Basin,Q958.8
  15. Clinical Significance of Hepatitis B Virus Core Gene PCR-RFLP Patterns on the Course of Hepatocellular Carcinoma and Liver Cirrhosis in China.,R735.7;R575.2
  16. Research on Micro-blogging Community-finding,TP393.092
  17. Genetic Variation in CD226 Gene and Systemic Lupus Erythematosus,R593.241
  18. Identifying and Clonging the cry30Ga2 Gene from B. Thuringiensis Isolate S2160-1,Q78
  19. The Community Structure of Iron-Reducing Geobacteraceae in Rice Rhizosphere under the Stress of Polycyclic Aromatic Hydrocarbons,X53
  20. Hyperspectral Unmixing Based on Nonnegative Matrix Factorization,TP751.1
  21. The Cloning and Expression Analysis of Mitochondrion atp9 Gene from Cotton (Gossypium Hirsutum L.),S562

CLC: > Environmental science, safety science > The basic theory for the Environment and Science > Environmental Biology > Environmental Microbiology
© 2012 www.DissertationTopic.Net  Mobile