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Isolation of Nitrifying Bacteria Adapted to Different Concentration of Nitrogen

Author: LuYunLi
Tutor: LiangYunXiang
School: Huazhong Agricultural University
Course: Fermentation Engineering
Keywords: Ammonia -oxidizing bacteria Nitrite -oxidizing bacteria Nitrification Separate
CLC: X172
Type: Master's thesis
Year: 2011
Downloads: 48
Quote: 0
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Abstract


The development of industrialization, urbanization intensifies, leading to more and more serious water pollution. Fix the problem of the water environment, more and more attention has been paid to how to take full advantage of nitrifying bacteria removal have become one of the hot waters of harmful nitrogen compounds. The relative industrial wastewater and domestic wastewater, low nitrogen content of natural lakes, the waters of a wide range of environmental impact factors making factory governance nitrifying bacteria is difficult to play a role. The subject it would be to the enrichment of natural water bodies, isolated adaptation, efficient low nitrogen concentration nitrifying bacteria can play a role in the nitrogen pollution in lakes. The characteristics of this paper, the solid attachment of nitrifying bacteria retain the continuous enrichment of the sediment carried two methods to separate the solid plate separation and MPN liquid separation, by detecting ammonia, nitrite, nitrate generation and concentration Strain selection. The main results are as follows: 1, separation results: The solid plate separation is not nitrifying bacteria, liquid MPN separation is better nitrification rates, sample 8 2, the initial ammonia concentration of 2mg / L, did not get ammonia The oxidation of the sample; the initial nitrite nitrogen concentration of 2mg / L, to obtain a nitrite oxidation the sample 1XN-1 within 5 days Water 2mg / L of nitrite nitrogen completely converted to nitrate . Initial ammonia concentration of 100mg / L to obtain a valid drop of ammonia samples 1XA-2 as the sole nitrogen source, ammonium nitrogen, 17 days 100mg / L ammonium nitrogen is converted to nitrate, the entire 1 nitrite accumulation in the process of nitrification 1mg / L; the initial nitrite nitrogen concentration of 100mg / L, nitrite oxidation samples 3, in order to reduce the amount of nitrite The nitrification rate, three samples 1SN-2 nitrification rate of 6.63 mg / L, D, 1Zn-2 nitration rate is 9.27 mg / L · d 1XN-2 nitration rate 11.23mg / L · d. 4, an initial ammonia concentration of 400mg / L, obtained ammonia oxidation Sample 2, but the resulting sample was reduced the ability of the ammonia concentration is low, a long time-consuming, not the actual value of the application; initial concentration of nitrite nitrogen is 400 mg / L, nitrite oxidation samples 3, to reduce the amount of nitrite nitrification rates, three samples 1SN-3 nitrification rate of 16.41 mg / L d 1ZN-3 the nitrification rate of 27.7 mg / L · d 1XN-3 nitration rate is 48.33 mg / L · d. Compare all strains nitrification characteristics, select 1XA-2 simulation sewage experiment. The results showed that with dosing 1XA-2 negative control dosing faster decline in ammonia water after 1XA-2 reduced nitrite concentration maximum, reduced nitrate accumulation, the whole process in half of the nitrogen is removed from the water. 1XA-2 to reduce natural water body ammonia certain role.

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CLC: > Environmental science, safety science > The basic theory for the Environment and Science > Environmental Biology > Environmental Microbiology
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