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Opticall Detection of Biomolecules Based on Microfluidics and Nanoparticles

Author: LiTangSong
Tutor: LiuShaoQin
School: Harbin Institute of Technology
Course: Biomedical Engineering
Keywords: Microfluidic Quantum dots Electrochemiluminescence Gold nanoparticles Silver mirror reaction
CLC: TP274
Type: Master's thesis
Year: 2011
Downloads: 77
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Abstract


The development of disease-related biological detection and application process, flux method for the detection, sensitivity, convenience raised higher and higher requirements. For example, the detection of nucleic acids, the conventional PCR method and apparatus is difficult to achieve high throughput, miniaturization of the detection; associated with the diagnosis of cancer the detection of tumor markers, very low content of the markers, the conventional methods such as radioimmunoassay, etc. used radioisotope, both damage to the human body and the environment, and the operation is complicated; Another example is associated with a variety of diseases of glucose detection, the traditional method is dependent on the enzyme, electrochemical, or fluorescent methods such as, enzyme easy deactivation electrochemical, and fluorescence method requires equipment complex and thus limits the application and promotion of such methods. In recent years the development of micro and nano technology, provide the opportunity to resolve the problem of the disease-related biological detection. In this thesis, the use of microfluidic technology and nano-materials, the development of several optical detection methods for nucleic acids, tumor markers and biological molecules such as glucose. The combination use of polydimethyl siloxane was prepared in parallel micro channels, with the nucleic acid of the loop-mediated isothermal amplification to detect the the influenza virus H1NA specific target sequence. When the pipeline in the presence of the target sequence, the target sequence of the amplification reactions result in a white precipitate, the white precipitate of magnesium pyrophosphate amplification reaction byproducts. Through the generation of the observed white precipitate sample yin can be judged. Also designed a fiber optic sensors combined microfluidic pipeline, by the change in turbidity of the test solution, real-time monitoring of nucleic acid loop-mediated isothermal amplification process. Compared with conventional nucleic acid detection in the PCR tube, the method has high throughput results intuitively advantages. Electrochemical luminescence properties of CdTe quantum dots, quantum dot labeled secondary antibody of AFP. Antibody modified for antigen another epitope on the ITO electrodes. Modified antibodies when the antigen is present, the antigen and the electrode, a quantum dot labeled antibody at the electrode surface to form a sandwich immune complex. Applying a voltage on the electrode, a quantum dot may generate electrochemiluminescence, light emitting intensity and the concentration of antigen, the lowest of the method capable of 10 ng / mL of AFP antigen respond. The paper also different concentrations of EDC / NHS marked effect of protein by high performance liquid chromatography were characterized. In addition, the paper also developed a gold nanoparticles assisted silver mirror reaction method for high sensitivity detection of glucose. When glucose is present in the system, the silver ions are reduced to elemental silver forming the silver sheath covering the surface of the gold nanoparticles, characterized solution of gold nanoparticles in the 520 nm absorption disappeared, characterized in enhanced absorption of 410 nm of the silver, the solution by red to yellow. The 410 nm absorption intensity and glucose concentration. The lowest with the naked eye to determine the presence of 10 nM of glucose, the linear range of 0.04 mM to 1.0 mM. Thesis, detailed discussion of the various factors affecting the method, and the method used for the detection of glucose in serum, with a good response.

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