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Since the 1980s the rise of microorganisms in addition to manganese technology, its effect, the advantages of low investment attention gradually. Microorganisms as a subject of ecological restoration, plays an important role in the heavy metal pollution. In this study, soil samples collected in Xiangtan the crane jinlingzhen manganese Luan tree recovery area, gain an advantage through step by step to improve the method of Mn2 concentration screening and domestication manganese-resistant strains, the dominant strains and manganese test, analysis and testing of the growth of the predominant strains curve, tolerance and related physiological and biochemical characteristics, and the predominant strains of taxonomic identification. The results are as follows: (1) screening three strong resistance manganese bacilli (2,3,80). In the low concentration range, Mn2 promoting effect on screening bacteria growth, but the point of concentration of the high Mn2, Mn2 inhibit cell growth. The strains resistant Mn2 performance the most tolerated, No. 80 bacteria. (2) minimum Mn2 concentration (16mg / L) in test point, highest No. 3 strains manganese removal rate (82.6%), followed by the No. 2 (72.3%), No. 80, the lowest (64.1%), Mn2 highest test concentration (about 2000 mg / L) point, the highest removal rates of bacteria, 80 (41.0%), followed by No. 2 (22.0%), the 3rd lowest (18.3%), in the Mn2 concentration of about 200mg / L points, the highest removal efficiency of a single strain and mixed bacteria sample Mn2 (gt; 90%). Strains mixing after each concentration point to maintain a high removal rate. (3) observed by colony morphology, Gram stain, 16s rDNA molecular identification: strain 2 Bacillus megaterium; strains 3 acid-producing Klebsiella; strains 80 Citrobacter freundii. (4) The screening strains around 18 ~ 21h basically reached growth peak of longer-term stability; 80 strains grown slower than the other two strains. 3 bacterial growth medium pH value increased, and which is conducive to the oxidation of Mn2 oxide precipitate is formed. The screening of the three bacteria to adapt to a wide range of pH values ??(5-9) in the temperature between 28 ~ 37 ℃ C, 2,3,80 strain optimum growth temperature were 35 ℃, 37 ℃, 33 ℃. Characteristics displayed by the scanning electron microscope shows that the three bacteria and manganese removal might Mn2 oxidation MnO2 precipitate. Antibiotic resistance test No. 3 strain resistant to ampicillin, the concentration of 200ug/ml also can grow, but kanamycin sulfate and chloramphenicol-sensitive. 2,80 Bacteria are sensitive to three antibiotics. (5) from the application point with peat as a carrier, to produce the screening strains probiotics.
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