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Molecular Cloning, Characterzation and Expression Analysis of ZmSKIP Genes in Maize

Author: NiuJinBo
Tutor: ChengBeiJiu
School: Anhui Agricultural University
Course: Genetics
Keywords: Maize ZmSKIP gene Genetic transformation Drought tolerance
CLC: S513
Type: Master's thesis
Year: 2012
Downloads: 7
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Abstract


Protein resemble SKIP are widely exist in eukaryotes, the members of whose family participate in including pre-alpha-RNA cut, RNA regulation, RNA degradation etc. RNA metabolic pathway, which play the indispensable role in growth and development of life.To plant stress response, relevant methods have been passed the detail research, but it has not been reported based on the study of the RNA control course.In this research, we cloned ZmSKIP gene of Maize, analysised by bioinformatics and operated the genetically modified drought resistance test. Main results are as follows:1.Clone SKIP homologus gene ZmSKIP in Maize.The total length is2308bp, and the length of ORF is1830bp, which coding609amino acid and a highly conservative SKIP SNW structure domain.2.Cloning SKIP gene from14Maize strains, with Bioinformatics and polymorphism analysis.As the result, ZmSKIP gene in the drought and drought resistance of the breed varieties with the quality difference basically drought differences have related in relationships.3.Constructing overexpression vector CPB-ZmSKIP, and double-strand RNAi vector ZmSKIPRNAi+2F+CPB.Transforming Maize by rhizobium agrobacterium-mediated transformation in situ. Using PCR method to detect purpose gene, to get the positive transgenic plants.4. Subcellular localization shows that, ZmSKIP protein was localized in nucleolus, being provided with RNA metabolic factors nuclear localization signal characteristics.5. Transgenic strains of drought resistance indexes are:SOD enzyme and the ABA hormones.Comparing with the wild type, we found that the transgenic plants drought resistance have obviously enhanced. It infers that ZmSKIP genes that can increase the Maize drought resistance, and it may participate in adversity adjustment process.

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CLC: > Agricultural Sciences > Crop > Cereal crops > Corn ( maize )
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