Dissertation > Excellent graduate degree dissertation topics show

Infection Survey and Immune Effect Evaluation of Classical Swine Fever in Part of Large Scale Pig Farm in Guangxi

Author: TanYingYi
Tutor: LuQinZhang
School: Guangxi University
Course: Preventive Veterinary Medicine
Keywords: Classical Swine Fever Clinical symptoms Sequenceanalysis Assessment of immune effect
CLC: S858.28
Type: Master's thesis
Year: 2012
Downloads: 62
Quote: 0
Read: Download Dissertation

Abstract


Classical swine fever (CSF) is the enemy that harm for swine industry in our country. Epidemic characteristics produced apparent change in strong immune pressure and various environmental changes under the influence of CSF in recent years. It is difficult to clinical diagnosis and prevention because CSF and other diseases caused by mixed infection that lead to pathological changes and clinical symptoms become more and more complex. The kind of pig CSFV sustained virus infection is the most headaches, it not only causes the serious barriers to reproduction, but also caused the death of newborn piglets of sows and immune tolerance, and virus infection has become one of the main causes in the spread of CSFV. Therefore, it is cann’t wait to carry out a herd CSFV has been purified.To tie in with the projects sponsored by the Ministry of Agriculture and the Ministry of Science and Technology on infection and immune effect evaluation of CSF in large-scale pig farms. The goal is to analysis the survey to pig swine fever and other major diseases clinical symptoms, pathological changes, virus infection and genetic variation analysis through to the Guangxi some large scale pig farm, and to further grasp the resent swine fever clinical and pathologic changes of the situation, at last to kind of CSFV infection situation. To formulate the control purification measures of CSF for the further. The main results are as follows:1. Through the portion of the487samples detected by pig, rate of CSF detection was11.91%; wherein, typical of atypical hog cholera swine fever accounted for18.18%, others accounted for81.82%; in the case of mixed infection by CSFV and PRRSV mixed infection accounted for21.21%.2.33head of CSF in anatomic pathology results show, at present on clinical lesions reductes to6cases, atypical CSF in majority. Atypical lesions in epiglottic cartilage surface pinpoint bleeding (17/27), the ileocecal valve local ulcer (16/27), myocardial petechial hemorrhage (11/27), renal pale, surface has the tip of the bleeding point (10/27), hyperemia bladder, bladder mucosa and mucosal bleeding spots (8/27) accounted for the majority, CSF the typical lesions of splenic marginal infarct (5/27), the ileocecal valve button necrosis rare (4/27). 3.15strains of CSFV E2gene sequencing and Genbank landing is completed. Through comparing Guangxi epidemic strains that discover their nucleotide homology is75.8%-100%; deduced amino acid homology is77.6%-100%, mainly for the gene I group and II group.2.1subsets virus and all strains were D25G variation widely appear in various subsets of strain that regularity of the variation in amino acids which compared with SHIMEN and HCLV strains.4. CSFV antibody detection in Guangxi7different dimensions pig farm of1814pig serum, found that antibodies general pass rate only83.13%(1508/1814), antibody levels are different in different scale field pig, and the antibody diversity is high, the average of diversity is up to35.48%, pig antibody level is too difference, which may lead to occurred in the presence of CSF.

Related Dissertations

  1. The Establishment of RT-PCR Assay for Detection of and Its Application in RHDV-Contaminated Classical Swine Fever Vaccine,S852.5
  2. Study on the E2 Gene Nucleotide Sequences and Pathogenicity of Prevalent Field Strains of Hog Cholera Virus,S852.651
  3. An expert diagnosis system for human cardiovas cular hemodynamics,R318.01
  4. Smokeless moxibustion treatment of functional dyspepsia spleen deficiency syndrome and clinical research,R246.1
  5. Surveillance and Diagnosis Report on Mixed Infection of Classical Swine Fever Virus and Porcine Circovirus Type 2,S858.28
  6. Epidemiology Investigation and Establishment of Diagnostic Method of Classical Swine Fever,S858.28
  7. Clinical Study of the Supplementing Qi, Nourishing Yin and Promoting Blood Flow Method in Treating Diabetic Nephropathy,R259
  8. Expression of the Glycoprotein E2 of Classical Swine Fever Virus and Preparation of Monoclonal Antibodies Against E2 Protein,S852.65
  9. Expression of Envelope Protein Gene E0 of Classical Swine Fever Virus and Prepartion of the Polyclonal Antibody Against E0,S852.65
  10. Detection of PCV2、PRRSV、CSFV、PPV、PRV、JEV、EMCV、PEV and PTV in Cases of High Swine Fever in Jiangsu Province in 2007 and Evaluation of TaqMan Real-time Fluorescent Quantitative RT-PCR to Differentiate Virulent CSFV Strains from Avirulent CSFV Strain,S858.28
  11. Clinical Analysis and Evaluate the Serum Anti-cTnI Antibody Levels of Patients with Hypertrophic Cardiomyopathy,R542.2
  12. Cloning, Eukaryotic Construction and Expression of Npro Gene of Classical Swine Fever Virus of Wuzhou Strain in Guang Xi,S852.65
  13. Proliferative Comparison of Chinese Attenuated Lapnized Strain on the Immortalized Swine Umbilical Vein Endothelial Cells and Bovine Testicular Cells,S858.28
  14. Chaperone Jiv90 for classical swine fever virus replication studies regulation,S852.65
  15. Genetic and Variation Analyses on CSFV E0 & E2 Gene in Parts of China and Sequence & Analysis of the Complete Genome of Epidemic Strains,S852.65
  16. Primary Selection microRNA Acting on CSFV in Swine Umbilical Vein Endothelial Cells,S858.28
  17. Classical swine fever virus field strains GICA method for detection of,S858.28
  18. Characterization of a Monoclonal Antibody Against Glycoprotein E2 of Classical Swine Fever Virus Isolate SM and Identification of Its Recognition Sites,S852.65
  19. The Study on the Theory of Five Viscera Correlation of Myasthenia Gravis,R277.7
  20. A Clinical Research for Early Intervention on Mild Cognitive Impairment after Cerebral Infarction by Ziyinjiannao Tablet,R277.7
  21. Research on Correlations of Changes of Cytokines Serum Concentrations and Clinical Symptoms in First Episode Schizophrenia,R749.3

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Livestock > Pig
© 2012 www.DissertationTopic.Net  Mobile