Dissertation > Excellent graduate degree dissertation topics show

Enhancement of the Immunogenicity of an Alphavirus Replicon-based DNA Vaccine Against Classical Swine Fever by Electroporation and Coadministration with a Plasmid Exressinorcine Interleukin2

Author: TianDaYong
Tutor: ChouHuaJi
School: Chinese Academy of Agricultural Sciences
Course: Preventive Veterinary Medicine
Keywords: classical swine fever alphavirus replicon-vectored vaccine electroporation interleukin2
CLC: S858.28
Type: Master's thesis
Year: 2012
Downloads: 38
Quote: 0
Read: Download Dissertation

Abstract


There are several advantages of the alphavirus replicon-based DNA vaccine pSFV1CS-E2againstclassical swine fever, such as self-replication, high-biosafety, high-level expression and so on. However,it is difficult to deliver the DNA vaccine into cells, so efforts should be made to enhance itsimmunogenicity. Electroporation (EP) is a technology to introduce macromolecules such as nucleicacids into cells via the application of brief electric pulses to induce transient and reversiblepermeabilization of the cell membrane. Interleukin2(IL-2) has been extensively used as adjuvant forDNA vaccines, for it is able to upregulate the magnitude and duration of immune responses.In this study, EP and a plasmid expressing porcine IL-2were used to improve the immunogenicityof pSFV1CS-E2. Pigs were immunized with pSFV1CS-E2alone or together with IL-2by EP or bysimple intramuscular injection. The results showed that EP combined with IL-2resulted in markedenhancement of E2-specific antibody responses. Moreover, CSFV-specific lymphocyte proliferation,IFN-γ and IL-4secretions were increased significantly in the pSFV1CS-E2+IL-2/EP group. Pigsimmunized with pSFV1CS-E2plus IL-2by EP were completely protected from lethal challenge, whichis comparable to the sterilizing immunity and full protection offered by the live attenuated vaccineC-strain and in contrast with the incomplete protection conferred by pSFV1CS-E2without or with IL-2or EP alone, as demonstrated by the presence of pathological changes or viral loads. In addition, theanimals immunized with this DNA vaccine could be distinguished from infected animals. We concludethat EP in combination with IL-2can significantly improve the immunogenicity of pSFV1CS-E2, whichis a potential marker vaccine against CSF.

Related Dissertations

  1. Electroporation Assisted Surface-enhanced Raman Spectroscopy for Living Cells,R318.51
  2. Improvement in Electroporation Transformation Efficiency of Gene for Bacillus Subtilis with Trehalose,Q78
  3. Detection Classical Swine Fever Virus and Porcine Circovirus Type 2 of Microarray Technique,S858.28
  4. Construction of Dnavaccine about Candida Albicans Hsp90 and Studies on Its Immunological Activity,R392
  5. Prokaryotic Expression of ISG15 and Polyclonal Antibody Preparation, and A Preliminary Study of the Interaction between ISG15 and CSFV,S852.651
  6. The Research of Situation of BVDV Infection in Swinery and Preliminary Analyze the Cause of BVDV Infection,S858.28
  7. PEF AT2R mediated gene expression in the vascular localized impact on neointimal,R541.4
  8. The Establishment of RT-PCR Assay for Detection of and Its Application in RHDV-Contaminated Classical Swine Fever Vaccine,S852.5
  9. Preliminary Research and Establishment of National Standards of Classical Swine Fever Lapinised Vaccine,S852.5
  10. Screening of Classical Swine Fever Virus NS2 Interaetive Proteins in Swine Umbilical Vein Endothelial Cell Line by Yeast Two-hybrid,S858.28
  11. Publishing a microRNA Catalog of Swine Endothelial Cells and Screenning Out microRNAs Involved in CSFV Infection,S858.28
  12. Thermophilic Bacillus Genetic Transformation of ethanol technology research,Q78
  13. Kallistatin electroporation -mediated gene expression in mouse models of IBD preventive treatment,S858.91
  14. Construction and Cell Transfection of the Targeting Vectors of Jiv90 Genes in Swine,S858.28
  15. The Experimental Study of Silencing Livin Gene by Transfecting shRNA Expression Vector into Pancreatic Cancer Sw1990 Cells with Electroporation,R735.9
  16. Primary Human Lymphocytes Transduced with HLA-A2 Restricted、ny-eso-1 Antigen-specific TCR Genes for Use in Adoptive Immunotherapy for Hepatocellular Carcinoma,R735.7
  17. Preparation of Anti-he4 Monoclonal Antibodies for Detection of Ovarian Cancer,R737.31
  18. Effects and Mechanism of pprI Gene Transfer by in Vivo Electroporation to Enhance the Radioresistance of Mice Exposed to γ Rays,R144
  19. Identification A Putative E2-binding Cellular Receptor of Classical Swine Fever Virus,S852.65
  20. Immunogenicity of A Recombinant Adenovirus Co-expressing Porcine Reproduction and Respiratory Syndrome Virus GP5 Protein and Classical Swine Fever Virus E2 Protein,S852.5
  21. Development of a Triplex TaqMan Real-time RT-PCR Assay for Quantitative and Differential Detection of Wild-type and HCLV Vaccine Strains of Classical Swine Fever Virus and Bovine Viral Diarrhea Virus 1,S854.43

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Livestock > Pig
© 2012 www.DissertationTopic.Net  Mobile