Dissertation > Excellent graduate degree dissertation topics show

Analysis of Differential Expressed Proteins in Mulberry (Morus alba L.) under Drought Stress

Author: HanSha
Tutor: JiaoFeng
School: Northwest University of Science and Technology
Course: Special Economic Animal Feeding
Keywords: flow cytometry drought stress two-dimensional electrophoresis
CLC: S888.2
Type: Master's thesis
Year: 2013
Downloads: 6
Quote: 0
Read: Download Dissertation


Mulberry is a perennial economic species which are widespread and have a goodadaptability to environment. In arid and semi-arid regions, drought stress is one of the mostimportant environmental stress factor limiting the growth and development of plant. Therelated molecular mechanisms of mulberry drought-resistant have an important meaning tocultivate drought-resistant varieties, increase the ability of drought resistance and amelioratethe ecological environment. In this study, we used flow cytometry to identify mulberry ploidy.It could lay the foundation for the study of mulberry gene dosage effect in the future; usingtwo-dimensional electrophoresis technology to analyze mulberry differential expressed proteinsunder drought stress. These drought resistance related proteins would contribute to theunderstanding on the mechanism of genes expression and regulation, it has an importantapplication value to improve the ability of stress resistance and promote the development of themulberry silk and ecological industry. This study was consisted of three parts:1. Flow cytometry identified mulberry ploidyThis is the first time researchers using a rapid and accurate technique to identify cellploidy of mutagenic mulberry polyploidy by using flow cytometry to detect large cell group. Inthis study, using flow cytometry identified three parts of the mulberry (leaf, bud and stem)ploidy. The results indicated that the ploidy of diploid and triploid is consistent with previousreports,403-1is a tetraploid,403-9is a triploid and the others are mixoploid which are diploidand tetraploid. This study established a rapid identification technique system of mulberryploidy. It can provide cytological evidence for studying molecular and physiologicalmechanism of mulberry autopolyploid material. Meanwhile, it will be more complicated toanalyze the mulberry polyploidy character development and gene dosage due to the mixoploid.2. The tissue culture of mulberry and changes of mulberry physiological indexes underdrought stressOptimized medium was used to breed diploid, triploid and tetraploid. Tissue cultures ofmulberry provided material for further research on gene dosage effect and the mechanism ofdrought tolerance in mulberry. This experiment used containing5%PEG6000Hoaglandnutrient solution to treat mulberries0,1,3,5,9,14days, respectively. After14days, using Hoagland nutrient solution to recover to20days. Then we determined the content of prolineand malondialdehyde.The results of physiological index changes showed that the droughtdegree of mulberry is deepening consistently as the extension of drought treatment time. Afterrewatered6days, mulberry plants got a drought relief, but not to the control physiologicallevels.3. The analysis on differential proteins of mulberry under drought stressWe used two-dimensional electrophoresis technology to analyze the changes of themulberry differentially expressed proteins under drought stress and found50proteins that aresignificant change (α=0.5) and the fold change is2by PDQuest software. Using0days as acontrol group, there are23up-regulation spots,21down-regulation spots and6fluctuation spotsin the treatment group. Using matrix assisted laser desorption ionization/time of flight/massspectrometry (MALDI-TOF–TOF) successfully identified35protein spots whichparticipate in stress defense, carbohydrate metabolism, protein metabolism and photosynthesisfour physiological process, respectively. There are three spots are unknown. The analysis ofprotein function shows that the improved drought-resistant of mulberry is by the way ofregulating the carbohydrate metabolism, photosynthesis and increasing the stress defenseprotein synthesis.

Related Dissertations

  1. Drought Stress on the Osmanthus Growth and Physiological Ecology Effect,S685.13
  2. Cellular and Proteomic Analyses of Endocytosis Mechanism Involved in Pollen-tube Growth in Nicotiana Tabacum,Q942
  3. Proteomic Analysis of Vibrio Alginolyticus in Response to Four Types of Antibiotics,S941
  4. Salt-Tolerance Evaluation of Brassica Napus Germplasm in the Bud and Seeding and Establishment of the Two-Dimensional Electrophoresis System,S565.4
  5. Effects of Gradually Drought on Physiological Indeces of Seeding in Tree Peony and Cloning with Sequence Analysis of Ga20-ox Synthesis of Tree Peony,S685.11
  6. Isollation and Identification of Haemophilus Parasuis and Its Proteomic Analysis under in Vitro Growth Conditions Mimicking the in Vivo Environment,S858.28
  7. Proliferation and Differentiation Effects of Different Culture Methods and Cell Factors on Mouse Spermatogenic Cells in Vitro,R329
  8. Effect of Drought Stress on Anatomical Structure and Physiological Characteristics in Solanum Nigrum,S649
  9. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  10. Study on the Cell Cycle of Liver Cells during Rat Liver Regeneration,Q253
  11. The Expression and Clinical Significance of TH17 Cells Related Cytokines in the Patients with Rheumatoid Arthritis,R593.22
  12. Analysis on the Function of Genes Involved in S-adenosylmethionine Metabolic Pathways to Drought Stress and Water Use Efficiency in Wheat,S512.1
  13. Responses of Three Kinds of Jasminum Sambac to Drought Stress,S685.16
  14. Physiological and Ecological Response of Soybean Seedlings to Drought and High Temperature under the Background of Climate Change,S565.1
  15. Responses of Native Shrubs to Drought and Salt Stress in Urumqi,S718.4
  16. Effects of Simulated Drought Stress with Polyethylene Glycol on the Seed Germination and Cutting of Jute and Kenaf,S563.5
  17. Responses of Ammodendron Argenteum (Pall.) Kuntze Seedings to Drought, Salt and Their Intercross Stresses,S792.26
  18. Study on Na~+ Accumulation and Salt Secretion of Tamarix Hispida,S793.5
  19. Screening of Differential Proteins in Different Parts of Pedicle Periosteum of Sika Deer,S825
  20. The Cloning of HARDY Gene from Arabidopsis and Expression in Tomato,S641.2
  21. Effects of Drought Stress on Physioecological Characteristics of Typical Plants in the Ecotone between Dry Valley and Mountain Forest,Q948

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Sericulture > Mulberry > Mulberry physical,ecological, biophysical, biochemical
© 2012 www.DissertationTopic.Net  Mobile