Dissertation > Excellent graduate degree dissertation topics show

Investigation on the Proliferation,Differentiation and Apoptosis Effect and Mechanism of LfcinB in Jurkat Cell

Author: HanZhaoLian
Tutor: XuXiaoZuo
School: Northeast Agricultural University
Course: Of Food Science
Keywords: LfcinB Jurkat cells Apoptosis P21waf1 Notch1 AKT
CLC: R151
Type: Master's thesis
Year: 2013
Downloads: 40
Quote: 0
Read: Download Dissertation


Lactoferricin B (LfcinB) is a microbial activity peptide of high antimicrobial activity, and hasextensive source. It has no effects to normal cells at concentration range of antineoplastic activity,but has pertinence that show cytotoxicity to most cancer cells of human. Synthetic LfcinB wasacted on acute T cell leukemia cells (Jurkat) in this experiment, and flow cytometry (FCM) andwestern blotting were used to study and discuss the effects and mechanisms of LfcinB to Jurkatcells on proliferation, differentiation and apoptosis.All this experiment has four parts:(1) Cell counting kit-8was used to measure the effect ofLfcinB to Jurkat cells on proliferation.100nmol/mL sirolimus as the blank group and LfcinB of0μg/mL,50μg/mL,100μg/mL,200μg/mL and400μg/mL as the experimental groups to measurethe proliferation ratios of Jurket cells. As the results, the effects of LfcinB on proliferation of Jurkatcells were enhanced by the increase of LfcinB concentration and action time and appeareddependence to concentration and action time.(2) Morphological variation of Jurkat cell nucleusthat was effect by LfcinB of0μg/mL,50μg/mL,100μg/mL,200μg/mL and400μg/mL after24hwas analysed through inverted flurescence microscopy. From the results, cell nucleus of theexperimental groups were concentrated and appeared dense dyed state, however, the blank groupswere appeared uniform dyed state. The cells of experimental groups were apoptosis.(3) Thechange rule of early apoptosis that LfcinB effect on Jurket cells was measured byAnnexinⅤ-FITC/PI double marker flow cytometry.100nmol/mL sirolimus as the blank group andLfcinB of0μg/mL,50μg/mL,100μg/mL,200μg/mL and400μg/mL as the experimental groupsto measure the apoptosis ratios of Jurket cells. According to the results, early apoptosis ratios ofJurkat cells were increased by LfcinB concentration at the same action time and at the same LfcinBconcentration when action time at72h, the early apoptosis ratio decreased obviously andproportion of late apoptosis or death cells was increased.(4) The changes of phosphorylated levelof P21waf1, AKT and mTOR of Jurket cells and the expressions of protein of Nocth1, C-MYC,Bcl-2, Bax and Cyclin D1. This experiment has eight parts which were100nmol/mL sirolimus,LfcinB of0μg/mL,50μg/mL,100μg/mL,200μg/mL and400μg/mL, sirolimus+PI3K inhibitor and LfcinB+PI3K inhibitor. The phosphorylation of P21waf1, AKT and mTOR of Jurket cells weredecreased by increasing of LfcinB concentration and prolonging of action time. And theexpressions of Nocth1, C-MYC, Bcl-2and Cyclin D1were negative correlation with the LfcinBconcentration and action time, but the expressions of Bax were appeared positive correlation withLfcinB concentration and action time.

Related Dissertations

  1. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  2. Expression of hnRNP A2/B1 and p21WAF1 Proteins in Lung Cancer and Their Clinical Significance,R734.2
  3. Therapeutic Effect and Mechanisms of Derived Mesenchymal Stem Cells in Intracerebral Hemorrhage Rat,R743.34
  4. Effect of Bufalin on Proliferation and Induce Apoptosis of Vascular Endothelial Cells EAhy926,R285
  5. The Effect of Bidentata Saponins on Early Osteoarthritis in Rabbit and PI3K/AKT Pathway,R285.5
  6. Effects of Dachengqi Decoction on the Expression Levels of Bcl-2 and Bax Gene of Smooth Muscle Cells of Small Intestine in Rat with Multiple Organ Dysfunction Syndrome,R285.5
  7. Effect of Chloride Channel Blocker on the Hippocampal Neuronal Apoptosis Induced by No in Culture,R363
  8. Expression of Caspase-4 and Caspase-3 Gene Related Apoptosis in Pathological Changes of Sternocleidomastoid of Congenital Muscular Torticollis and Four Years Clinical Followed-up for Opereated Patients,R726.8
  9. The Relationship between CEF/TE Neo-adjuvant Chemotherapy, MAPK/PI3K Signal Pathway and Ki-67 in Breast Cancer,R737.9
  10. Expressions and Relationships of PI3K/AKT and Apoptosis Related Regulation Factors in Epithelial Ovarian Tumors,R737.31
  11. Effects of bFGF on p-Akt (Ser473) and Fractalkine Expression in Cerebral Ischemia/Reperfusion of Rats,R743.3
  12. Pathological Changes of Cerebral Vasospasm and the Expression of Cell Apoptosis Related Factors JNK and AKT Following Subarachnoid Hemorrhage of Rats,R743.34
  13. Expression and Significance of p21WAF1、Gadd45a and p53 Protein in Breast Cancer,R737.9
  14. Effects of Sevoflurane Preconditioning Delayed Protection on Cardiomyocyte Apoptosis in Rats with Myocardial Ischemia/Reperfusion Injury,R965
  15. Study on Cytotoxicity, DNA Damage and Repair Induced by Phenol in Vitro,R114
  16. Eualuation of Acute Toxicity of Liu-She-Wan in Mice and Its Antitumor Effects in Hep3B Cells,R285.5
  17. Effect of Resveratrol on Intestinal Mucosal Permeability in Rats after Intestinal Ischemia-reperfusion Injury,R285.5
  18. Experimental Study of Expression of XAF1 Following Lung Contusion in Rabbit,R655.3
  19. Effects of Combined Therapy of 5-FU and Inhibitor of ClassⅢ PI3K Human Gastric Cancer SGC7901 Cells,R735.2
  20. Expression of AKT and PTEN Protein and Their Relationship with Clinic Characteristics of Esophageal Carcinoma,R735.1
  21. Effects of Suifotanshinone Sodium on the Growth of SMMC-7721 Hepatoma Cells,R735.7

CLC: > Medicine, health > Preventive Medicine,Health > Nutrition, hygiene,food hygiene > Nutrition
© 2012 www.DissertationTopic.Net  Mobile