Dissertation > Excellent graduate degree dissertation topics show

Effects of Methionine Hydroxy Analogue on Intestinal Redox Status and Bone Development of Broilers

Author: ZhaoZuo
Tutor: ShiYongZuo
School: Jiangnan University
Course: Nutrition and Food Hygiene
Keywords: methionine hydroxy analogue growth redox status intestine bone
CLC: R151.1
Type: Master's thesis
Year: 2013
Downloads: 4
Quote: 0
Read: Download Dissertation


Intestinal and bone are important organs for digestion, absorption and pillar, It is crucial tomaintain their healthy and steady-state. Methionine(MET) is an essential amino acid, itsalternative source including2-hydroxy-4-methylthiobutanoic acid (HMTBA) and2-hydroxy-4-methylthiobutyric acid calcium salt(HMTB-Ca). Due to their structuraldifferences, compared to DL-methionine(DLM), HMTBA and HMTB-Ca may have strongantioxidant activity to play a positive role in intestinal oxidation and bone growth. Theantioxidant activity of DLM, HMTBA and HMTB-Ca in vitro was compared; Broilers werechosen as the object for their short growth cycle and high metabolic, the effect of differentlevels of methionine sources on growth performance, nutrient digestion and absorption,intestinal oxidation status and bone development were study, and its possible antioxidantmechanism was explored.In vitro: the differences of clearance rate of the three met sources on O-2· free radical andthe liver lipid peroxidation inhibition were examined; In vivo:2101-day-old male rosebroilers were randomly divided into7groups: control group (no exogenous methionine add),and other groups added with DLM, HMTBA, HMTB-Ca, and with0.2%/0.3%(first stage),0.1%/o.2%(last stage). At21and46day, growth performance, nutrient digestibility, digestiveenzyme activities, intestinal morphology, intestinal redox state, and bone developmentindicators and mRNA expression levels of intestinal and bone by RT-PCR were measured.The main results of the paper are as follows:(1)In vitro: O-2·scavenging capacity:HMTBA>DLM>HMTB-Ca; liver lipid peroxidation inhibition rate: HMTBA>HMTB-Ca>DLM.(2)At21day and46day, daily gain and feed intake were improved in chicks fed threemethionine sources and were positively correlated with the dose; Compared with the DLM,daily gain and feed intake at21day and daily gain at46day were significantly improved inbroilers fed HMTBA and HMTB-Ca (P<0.05), the high level of HMTBA and HMTB-Cagroups are of the most significant.(3)Compared with the DLM group, the intestinal amylaseand lipase activity were significantly enhanced in the high level of HMTBA and HMTB-Cagroups, the substance apparent digestibility were significantly enhanced in HMTB-Ca group,the ratio of villus length/crypt depth in duodenum and jejunum were significantly increased inbroilers fed high dose of HMTBA and HMTB-Ca (P<0.05), somatostatin (SS) was reduced at46day.(4)The GSH/GSSG ratio and CAT activities were significantly increased in broilersfed HMTBA and HMTB-Ca(P<0.05), MDA levels were decreased in duodenum andjejunum(P<0.05); Antioxidant genes NRF1, SOD1, SOD2, GST, PGC-1α, MT1mRNAexpression levels were raised in duodenum and jejunum.(5)Compared with the DLM, tibiaand femur bone strength and bone index were significantly improved in chicks fed high dosesof HMTBA and HMTB-Ca(P<0.05), calcium and phosphorus content in tibia and femurwere significantly improved, key genes of bone decomposition MMP2, MMP9mRNAexpression levels were significantly lowered in broilers fed HMTB-Ca(P<0.05).

Related Dissertations

  1. Expression Survey of Key Factors of Canonical Wnt Signalling Pathway in the Human Embryonic Tooth Germ,R78
  2. Cloning and Expression of Growth Hormone Gene in Cobia (Rachycentron Canadus),Q786
  3. Effects of Nitrogen Fertilizer on Morphological Development of Alfalfa Root,S541.9
  4. Effect of High Level of Copper and Zinc on Growth Performance and Metabolism and Its Content Level in Serum and Deposit in Tissues of Weanling Piglet,S828.5
  5. Effects of Low-protein Diet on Production Performance and Feeding Environment of Piglet,S828.5
  6. Study on the Trophic Value of Blood Meal by Different Processes and the Effects of Supplements in Broiler Chicken,S831.5
  7. Study on the Separation and Purification Way of Arctiin from Fructus Arctii and the Effect of Arctiin on the Growth of Mice C2C12 Cells,S853.7
  8. The Effect of Vitamin E and Vitamin C on Growth Performance, Non-specific Immune Response for Cobia, Rachycentron Canadum,S963.1
  9. Effects of Dietary Oil Sources on Growth Perfomance, Lipid Deposition and Blood Biochemical Index of Carassius Auratus Gibelio,S963.1
  10. Effects of Shading on Growth and Nitrogen Metabolism in Cotton Plant (Gossypium Hirsutum L.) during Flowering and Boll-Forming Stage,S562
  11. Responses of the Double-Cropping Rice Population Growth and Nitrogen Uptake to Density and Nitrogen Management and the Diagnosis Based on NDVI,S511
  12. Research on Characteristics of Growth, Development and Material Metabolism of Flue Cured Tobacco Leaves in Huili, Sichuan,S572
  13. Effect of Extremely Low Protein Diet to Meishan Gilt on Its Farrowing Performance and Characteristics and Immunity of Its Offspring,S828
  14. The Effects of Isoflavones in Soybean Straw on the Growth and meat Quality of Goat,S826.5
  15. Nutritional Evaluation and Feed Effects of Solidago Canadensis in Rex Rabbits,S829.1
  16. Effects of Chinese Medicine Herbs Jiegusan on Fracture Healing in Dogs,S858.292
  17. Studies on Fermentation of Unconventional Protein Feeds and Their Application in Broiler Diets,S831.5
  18. Effects of the Cyclic Starvation-Refeeding on Growth Performance, Body Composition, Digestive Enzymes and Antioxidant Enzymes of Megalobrama Amblycephala Yih and Cyprinus Carpio Var.Jian,S917.4
  19. The Construction and Evaluation of a Novel Non-viral Gene Transfection System and Its Application in Mesenchymal Stem Cells Gene Recombination,R346
  20. The Study of Rabbit Bone Marrow Mesenchymal Stem Cells Separation Culture and Directional Differentiation,R329
  21. Analysis of Nutrition and Weight Gain in Very Low Birth Weight Infants during Hospitalization,R722

CLC: > Medicine, health > Preventive Medicine,Health > Nutrition, hygiene,food hygiene > Nutrition > Nutritional physiology
© 2012 www.DissertationTopic.Net  Mobile