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Small molecule fluorescent probes because of high sensitivity, good selectivity and detection in the field of biologically active species is widely used, is detected by optical analysis of biologically active substances most commonly used, one of the most important tools. Fluorescent dyes based on the traditional fluorescent probe according to the different dye precursor each have advantages, but as the sensitivity of the active species, selectivity requirements increase, these fluorescent dyes in the matrix as the fluorescent probes are also some shortcomings. Example, cyanine fluorescent probes for the mother Stokes shift is small, the quantum yield is not high, but the probe will polymerize photobleaching, broken keys decomposition phenomena; based rhodamine, BODIPY, fluorescein and incense coumarin dyes etc. fluorescent probe relatively high fluorescence quantum yield, and a better optical stability and chemical stability, but these dyes are generally in the emission wavelength of the short wavelength area, prone to spontaneous fluorescence and scattering biological systems light interference, and the Stokes shift is not great. Therefore, the above characteristics of various types of fluorescent probes, it is necessary to design better performance fluorescent dye, in order to meet the requirements of the actual biological detection. Currently on the synthesis of novel fluorescent probes, mainly in the following areas to improve: 1. Improve probe fluorescence quantum yield, increasing the detection sensitivity; 2. Increased Stokes shift, in order to reduce the self-absorption of the dye and spectral interference; 3 red shift of the emission wavelength of the dye, increased tissue penetration, avoiding certain substances in biological systems (water, liposome, protein, etc.) of light absorption, reducing noise ratio; 4. probe mother's light stability, less prone to polymerization or photobleaching; 5. probe good water solubility, good penetrating, low toxicity to organisms. Another chemiluminescent emission type probe as the probe, because no excitation light source, transient response, high sensitivity detection in biological applications to a certain degree. Chemiluminescent detection using the energy released by a chemical reaction in the reaction system excitation fluorescent substance, the chemical energy is converted into radiation, the process without the excitation light source, eliminating the interference of background light, it is suitable for in situ detection of living organisms and vivo imaging. However, the current probes chemiluminescence quantum yield is generally not high, and the emission wavelength in a shorter wavelength region, further improve the detection sensitivity and selectivity. Therefore designed with new luminescent mother, emission wavelength in the far visible - near infrared chemiluminescence small molecule probes will further broaden the chemiluminescence detection method in biological Analysis. Acid-base balance and the intracellular redox balance is important biochemical reaction equilibrium of cell physiological processes play an extremely important role in the regulation. Cell acidity (pH) such as cell growth, cell endocytosis, cell adhesion plays a key processes such regulation, cell metabolism is one of the important parameters. Many reported that a number of diseases and in the cytoplasm or the pH of acidic organelles abnormalities. Reactive oxygen species (reactive oxygen species, ROS) involved in cellular signal transduction, neurotransmission, immune system control, regulation of cell growth and cell metabolism, cellular redox balance system is an important player. Therefore, an accurate determination of intracellular pH and active oxygen concentration is very important. Fluorescent probe method has high sensitivity and selectivity, and the use of fluorescent probes confocal imaging technology can achieve change monitoring intracellular acid-base balance and the temporal and spatial distribution of the proton. Chemiluminescence reaction with free radicals and transformation are closely related, so the chemiluminescence detection probes in reactive oxygen species has a unique advantage. In this paper, fluorescent, chemiluminescent characteristics of these two types of optical probes to two fluorescent boron fluoride matrix, synthesized two pH fluorescent probe and a small molecule chemiluminescent probes. In order to achieve a fluorescent probe fluorescence imaging of intracellular pH. Preliminary tests using a chemiluminescence probe the superoxide anion radical. Specific tasks include the following four aspects: a. Introduced fluorescent, chemiluminescent probes in biological detection progress, focusing on the two compounds of boron fluoride probe the structural characteristics, physical and chemical properties and optical sensing applications were reviewed. . Two. Synthesis of a structure to difluoroborane maternal pH reversible \The probe maximum excitation and emission wavelengths were at 465nm 608nm office, Stokes shift up to 143nm. A mercapto group as the probe in response to a group, between the pH = 4.0-8.0 was sensitive response, the fluorescence intensity enhanced with the increase of pH; detailed study of the response of the principle of the probe, the probe light stability found, has good penetrating properties to which the probe is to achieve a pH within the human hepatoma cell fluorescence imaging analysis. Three. Synthesis of a structure to difluoroborane mother, pyridine groups in response to the pH of fluorescent probes. The probe pH = 3.0-6.0 range was sensitive response to the probe to achieve a pH within the human hepatoma cell fluorescence imaging analysis. Fourth, the synthesis of a structure with two boron fluoride precursor, MCLA analogues reactive group chemiluminescence probe for aqueous detection of superoxide anion radicals. Of the probe in dichloromethane in the maximum emission wavelength of about 645nm, is a visible region on the far chemiluminescent probes.
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