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Vivo there is a variety of functional cation, plays a vital role in the organism's various physiological processes and pathological processes. Such as metal iron ions directly involved in oxygen transport and storage, to participate in a variety of metabolic processes of the organism, and ferrous ions can catalyze the active species in the body to produce high concentrations of harmful hydroxyl radicals. Zinc ion activity concentration is too low will cause neuronal damage, and apoptosis. However, if the zinc ion content is too high, it will cause zinc poisoning. Therefore, it is possible to be detected in living cells of these functional cation has important medical and biological significance. Fluorescence method with high sensitivity, high selectivity, easy and quick, real-time in situ detection, etc., so that it has a broad application prospects in the field of environmental science, analytical chemistry and life sciences. Design of high selectivity, high sensitivity, and quickly identify and regional positioning vivo functional cation novel fluorescent probes, timely and accurate detection of functional cations of different cells, then a more detailed understanding of cation The biological function of these functional cation mechanism of action, so that the more sophisticated and profound. Research cationic fluorescent probe for the detection function, we carried out the following work: (a) the design and synthesis of a new fluorescent probe ratio the BDP-Cy-Tpy to detect changes in the concentration of ferrous ions in living cells. The probe is composed of two parts, one part is the fluorophore BODIPY, another part of the ferrous ions is insensitive to the specific response containing ferrous ions the fluorophore cyanine (Cy) of CY-Tpy. When the presence of ferrous ions, containing cyanine part of the formation of Fe 2 -the TPY complex will cyanine occurrence of photoinduced electron transfer (PET) effect, resulting in the fluorescence quenching, and BODIPY parts of the fluorescence intensity is constant. The experiment proved probes BODIPY, Cy-Tpy ratio of fluorescence intensity and the concentration of iron ions showed a good linear relationship. The BDP-Cy-Tpy having high selectivity and sensitivity, and can instantly respond to changes in the concentration of ferrous ions. And the probe for the first time achieved by fluorescence ratio imaging method to visualize the imaging of cells within the internal and external ferrous ion concentration, to obtain a satisfactory result. Therefore, the the ratio fluorescence probe also provides a potential method for the clinical study of the physiological function of the ferrous ion, and also provides a basis for the feasibility vivo functional cation ratio fluorescence assay.
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