Dissertation > Excellent graduate degree dissertation topics show

Studies on Chemical Constituents of Acorus Calamus Linn

Author: QiaoDi
Tutor: ZhouChangXin; GanLiShe
School: Zhejiang University
Course: Of Pharmacy
Keywords: Acorus calamus Linn. Sesquiterpenes Phenylpropanoids Lignans Antidiabetic
CLC: R284
Type: Master's thesis
Year: 2012
Downloads: 33
Quote: 0
Read: Download Dissertation


Acorus calamus Linn. is a member of Acorus genus, Araceae family. The dry rhizome of Acorus calamus is used as "Zangchangpu" in Triditional Chinese Medicine.In this thesis, chemical constituents from70%ethanol extract of Acorus calamus were studied. On the basis of different kinds of modern chromatography techniques (silica gel, ODS, HPLC and Sephadex LH-20) and spectroscopic methods (MS,1H-,13C-NMR),10compounds were isolated from Acorus calamus and identified as:1β,4β,7α-trihydroxyeudesmane (1), bullatantriol (2), teuclatriol (3), threo-1’,2’-dihydroxyasarone (4), erythro-1’,2’-dihydroxyasarone (5),(+)-de-4’-O-methyleudesmin (6),(+)-de-4’-O-methylmagnolin (7),(+)-eudesmin (8),(+)-magnolin (9),β-sitosterol (10). Compounds1,2,4-9were isolated from Acorus calamus for the first time. Compounds1,2,6,7,8,9were obtained from Acorus genus for the first time.Meanwhile, in vitro antidiabetic activities of the extracted fractions were investigated using3T3-L1cell line. In the adipocyte differentiation assay, compared to the solvent control group, lipid droplets in the ethyl actate and water fraction groups increased significantly, similar to the rosiglitazone positive control group. Glucose consumption of the n-butanol fraction group and rosiglitazone group increased obviously. In the ethyl acetate fraction group and70%ethanol extract group, the glucose consumption increased significantly.The antidiabetic activities of the isolated compounds were evaluated on HepG2 cell model of glucose consumption. Glucose consumptions in1μg·mL-1,5μg·mL-1, and25μg·mL-1groups of compound1,3,6,7,8increased significantly with dose-dependence (P<0.05, P<0.01or P<0.001), and all the increasing rates were greater than20%. The glucose consumption increased83.4%in the positive control group (metformin) at1mmol·L-1. The result suggests that the sesquiterpenes and lignans might be responsible for the antidiabetic activity of Acorus calamus.In conclusion, this research has provided the theoretical and experimental basis for the development and quality control of Acorus calamus.

Related Dissertations

  1. Study on Antioxidant Activity of Secoisolariciresinol Diglusoside,R285
  2. Studies on Chemical Constituents of Schisandra Chinensis Baill,R284
  3. Studies on the Active Constituents of Larix Olgensis Henry var. Koreana Nakai,R284
  4. Study on the Total Synthesis and Bioactivities of Syringaresinol and Glycosides,TQ463.2
  5. Mangiferin Zhimu Isolation and structural modification,R284
  6. Studies on Chemical Constituents of Leaves of Piper Laetispicum C.DC,R284
  7. Study on the Pharmakognosie and Development of Caulis of Schisandra Chinensis (Turcz.)baill,R282.71
  8. Studies on the Active Constituents of Zanthoxylum Nitidum (Roxb.) DC.,R284
  9. Total Synthesis of Some Naturally Occurring Lignans and Studies on Cross-coupling Reactions of Some Phenylpropanoids,O621.25
  10. Studies on the Regionselective and Stereoselective Synthesis of Some Naturally Occurring Lignans,O629
  11. Studies on the Antidiabetic Components of Acorus.calamus Linn and Studies on the Flavonolignans of Silybum Marianum (L) Gacntm.,R284
  12. Preparation of Sinapyl Alcohol Derivatives & Neolignans and Evaluation of Their Cytotoxic Activities,R914
  13. Structure Idetification of Phenylpropanoids Compounds from Geum Aleppicum Jacq.,R284
  14. Study on Evaluation and Germplasm of Schisandra. Chinensis(Turcz.)Baill,R282.71
  15. Study on Extraction, Isolation and Antioxidant Activity of Lignans of Sesame Cake,TS229
  16. The staghorn fern chemical composition and biological activity of,Q949.36
  17. Study on the Extraction Technology of Lignans in Fructus Schisandrae Chinensis(Shisandra Chinensis Baill)and Bacteriostasis of the Extraction,TQ461
  18. The Study on Secondary Metabolites of Serissa Serissoides and Hepatic Protection Activity,R285
  19. Two kinds of chemical constituents of medicinal plants,R284.1
  20. Study on Bioactivity and Chemical Component of Two Species of Plants of Meliaceae,S482.3
  21. Isolation of Flaxseed Lignans and Effect of IT on the Human Breast Cancer Xenografts in Nude Mice,R737.9

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical
© 2012 www.DissertationTopic.Net  Mobile