Dissertation > Excellent graduate degree dissertation topics show

Study on the Mechanism that Icaritin Inhibits Proliferation of HepG2Cells

Author: HuangJiaBin
Tutor: ChenMingJie
School: Huazhong University of Science and Technology
Course: Biochemistry and Molecular Biology
Keywords: Icaritin HepG2cell line cell cycle JAK2/STAT3
CLC: R735.7
Type: Master's thesis
Year: 2012
Downloads: 39
Quote: 0
Read: Download Dissertation


Hepatocellular carcinoma (HCC) is one of the most common malignant tumorsworldwide. China is the hardest hit of liver cancer, and the highest mortality of livercancer is happened in China.Therefore study on the treatments and drugs to liver cancer isof great significance in China. Epimedium Genus which has long been used in Chinesetraditional medicine has many pharmacological activities such as enhancement ofosteoblastic and cardiac differentiation. Icaritin, a prenylflavonoid derivative from theChinese herb Epimedium, possesses various activities, including cardiovascular protection,osteoblastic enhancement, the immune system regulation, as well as inhibition of humancancer cells growth. However, the function and the underlying mechanisms of icaritinrestrainment on cell growth have not been well discovered. In this study, we used HepG2cell line, a kind of human hepatocellular carcinoma cell line, as test material, in order toexplain the aititumor mechanism of icaritin by using the molecular biology methods suchas MTT, RT-PCR and Western blot. The results are as follows:(1) Icaritin inhibited the proliferation of human HepG2cell in the doses and timedependent ways. MTT assay showed that the higher the drug concentration, the strongerthe inhibition on cell growth, which means that icaritin inhibits the proliferation of cancercells in a dose-dependent manner.(2) Icaritin induced S arrest in cell cycle progression. HepG2cells were treated withicaritin of gradient concentrations for24h, Cell cycle analysis by flow cytometry, showedthat icaritin induced an obvious S arrest. Icaritin treatment down-regulated the expressionlevels of cyclin A and CKD2which were the key genes of S phase. Expression assay ofp53and p21by Western blot analysis showed that icaritin induced apoptosis of HepG2Cells.(3) The mechanism of icaritin inhibition on HepG2cell growth is to inhibit theexcessive activation of p-JAK2and p-STAT3. mRNA levels of JAK2and STAT3weredetected with RT-PCR, and the expression levels of p-JAK2and p-STAT3were assayed byWestern blot.The results showed that icaritin treatment down-regulated mRNA levels ofJAK2and STAT3and inhibited the activation of p-JAK2and p-STAT3.It means that icaritin inhibits the proliferation of HepG2cells through curbing the excessive activationof JAK2/STAT3signaling pathway.Through this study we found that icaritin has significant inhibitive effect on HepG2cells, which may provide a new way for the development of new effective drugs on livercancer.

Related Dissertations

  1. Study of Oridonin on SGC-7901 Cell Proliferation by Inhibitting Cell Cycle Proteins,R285
  2. Expression of Prolyl Isomerase Pin1 in Osteosarcoma and the Effect of Regulation on Cell Cycle,R738.1
  3. Heme Oxygenase is Involved in Salinity-Induced Tomato Lateral Root Development and Its Relationship with Hydrogen Peroxide,S641.2
  4. The Involvement of Heme Oxygenase/Carbon Monoxide System in Cobalt Chloride-Induced Lateral Root Development in Tomato Seedlings,S641.2
  5. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  6. Study on the Cell Cycle of Liver Cells during Rat Liver Regeneration,Q253
  7. Effects of Different Immunization on the Development of Immune Organs,cell Proliferation and Expression of Apoptosis-relatived Gene in Chicken,S858.31
  8. Influences of Temperature on SCF/c-kit Prolifertation Pathway and Cell Cycle of Rat Spermatogonial Cells Cultured in Vitro,R698
  9. Expression and Significance of p21WAF1、Gadd45a and p53 Protein in Breast Cancer,R737.9
  10. Effects of Rpb5-mediating Protein (RMP) on Cell Proliferation of Hepatocellular Carcinoma Cells,R735.7
  11. Preventive Effects of Low Intensity Microwave on Damages of Cells in Hematopoietic System Induced by γ-rays and Doxorubicin Hydrochloride,R733.71
  12. Expression of Exogenous HSP70 Gene in Human Lung Cancer A549 Cells and Its Effect on the A549 Cells Growth,R734.2
  13. The Study on the Radiosensitizing Effect of Artesunate on Nude Mouse Transplanted with HeLa and Siha Cells of Cervical Cancer,R737.33
  14. Radiosensitizing Effect of Cathepsin L on Glioma Stem Cells,R739.4
  15. Study on Anticancer Effect of Two Novel Topoisomerase Inhibitors and Their Mechanisms,R96
  16. Inhibitory Effect of Ginsenoside Rb1 on Proliferation of Vascular Smooth Muscle Cells and Carotid Neointimal Hyperplasia Induced by Balloon Injury,R285.5
  17. PTPRO Negatively Regulates Cell Cycle Progression by Targeting E2F1 through c-Abl,R363
  18. The Effects of Hypoxia on Development of Ovine Fetal Liver Cells and Its RAS Mechanisms,R363
  19. Study on the Role of LyGDI Regulated the Rac1 in the Apoptosis of K562 and HL-60 Cells Induced by 60 Coγ Rays,R363
  20. The Study on the Radiosensitizing Effect and Mechanism of Artemisinin on Nude Mouse Transplanted with HeLa and Siha Cells of Human Cervical Cancer,R737.33
  21. Activation of RASSF2 by p300 Induces S/G2 Cell Cycle Arrest in Gastric Cancer Cell HGC-27,R735.2

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
© 2012 www.DissertationTopic.Net  Mobile