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Effect of Bushen Quhan Zhi Wang Tang on the expression of VEGF and BMP-2 in CIA rats

Author: ZhangCui
Tutor: WangWeiGang
School: Beijing University of Traditional Chinese Medicine
Course: Chinese and Western medicine combined with medical
Keywords: BMP-2 Nourish Kidney Dispel Cold Cure Wangbi Decoction Rheumatoid Arthritis VEGF
CLC: R285.5
Type: Master's thesis
Year: 2012
Downloads: 55
Quote: 0
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Abstract


OBJECTIVE:Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease characterized by pathologic changes of the joint.It is clinically manifested as symmetric arthrophlogosis mostly with joints of hands,wrists and feet involved.At the initial stage,there may be redness,swelling,a hot sensation,pain and limited motion in the affected joints,while at the advanced stage,it would result in articular cartilage and bone destruction,rigidity and deformity of joints may be seen.Professor Jiao Shude had the rich traditional Chinese medicine (TCM) clinical experience over the years, named the RA Wangbi, combined TCM etiology, pathogenesis, syndrome and treatment with modern medicine of knowledge created the decoction in the treatment of RA,nourish the kidney dispel cold cure Wangbi,and achieved good curative effect. But this side of the evidence-based medicine curative effect and basic medicine are not perfect, this study tests and reviews concern the TCM and modern basic medicine, and discuss the effect of nourish kidney dispel cold cure Wangbi decoction in treatment of RA.Syndrome differentiation and therapeutic principle of nourish kidney dispel cold cure Wangbi decoction is nourish kidney eliminating cold, dispelling wind removing dampness, activate blood circulation and collaterals, strong bones and muscles.After years of traditional Chinese medicine clinical practice has proved the decoction’s curative effect.The test on the study of nourish kidney dispel cold cure Wangbi decoction treatment.The model of collagen induced arthritis(CIA) rats has successed.The test to observe the therapeutic effect of nourish kidney dispel cold cure Wangbi decoction of every dose group to CIA rats, and to detect vascular endothelial growth factor (vascular endothelial cell growth factor, VEGF) level of CIA rats,to check bone morphogenetic protein2(Bone morphogenetic protein-2, BMP-2) level of bone tissue and synovial organization.In conclusion,the purposes of the research are that evidencing the treatmentprinciple of nourish kidney dispel cold cure Wangbi decoction by the thinking and methods of integrative western medicine and TCM;There are else two purposes,one is to discussing the therapeutic effect of every different dose size of nourish kidney dispel cold cure Wangbi decoction. further analysising of the prescriptions of the decoction in treatment of RA.;Another is to confirming wheather nourish kidney dispel cold cure Wangbi decoction of every dose group could detect vascular endothelial growth factor (vascular endothelial cell growth factor, VEGF) level of synovial organization and bone tissue of CIA rats,and could increase bone morphogenetic protein2(Bone morphogenetic protein-2, BMP-2) level of synovial organization and bone tissue of CIA rats.METHOD:74male Wistar rats160g-180g were randomly divided into6groups:normal control group,model group,methotrexate group(MTX group),nourish kidney dispel cold cure Wangbi decoction concentration dose group(high-dose group),nourish kidney dispel cold cure Wangbi decoction standard dose group(middle-dose group), nourish kidney dispel cold cure Wangbi decoction dilution dose group(small-dose group).The Cow type Ⅱ collagen was dissolved in0.1mol/L acetic acid, placed in4℃refrigerator overnight.Mixed all the medicament fully according to2mg/ml of Ⅱ collagen with Freund’s complete adjuvant emulsion is1:1.Except the normal group, eale each rats proceeded200ug Ⅱ collagen tail subcutaneous injection.A week later,except the normal group,proceeded the booster immunization,each rats injected100ug Ⅱ collagen tail subcutaneous injection.So that successfully manufactured collagen-induced arthritis rat model.Each drug group separately treat for28days to observe the drug’s therapeutic effect. After the treatment cycle,intercepting the knee joint,mesured the level of BMP-2and VEGF in bone tissue and synovium by Reverse Transcription-Polymerase Chain Reaction(RT-PCR)RESULTS:1. Compared with model group, every dose of nourish kidney dispel cold cure Wangbi decoction could decrease arthritis index score,and had significant difference (P<0.01);compared with MTX group, arthritis index score of middle-dose group decreased significantly (P<0.05), arthritis index score of small-dose group and high-dose group of no significant difference (P>0.05); Between Chinese traditional medicine group, middle-dose group had the best effect to Arthritis index scorc,Compared with small-dose group and high-dosage group has significant difference (P<0.05); The effect of small-dose group better than high-dose group; high-dose group has the worst effect in three Chinese traditional medicine groups.2. Model group BMP-2mRNA express increased, CIA rats could produce BMP-2to repair bone damage。Compared with control group, every dose of nourish kidney dispel cold cure Wangbi decoction and MTX group could increase BMP-2mRNA level;Compared with MTX group, BMP-2mRNA expression level of middle-dose group increased significantly Methotrexate group, the small-dose group and large-dose group of BMP-2mRNA expression level difference is not much. Between Chinese traditional medicine group, middle-dose group had the best effect to BMP-2mRNA level, and compared with small-dose group and high-dosage group has significant difference; The effect of small-dose group better than high-dose group, but far less than middle-dose group but no significant difference.3. VEGF mRNA express of cotrol group compare with model group, expression was significantly elevated expression levels of VEGF mRNA in model group.The CIA rats model was more successful, causing the lesions of rats similar to human rheumatoid arthritis, and VEGF expression levels, indicating that lesions more serious increased. Compared with control group, every dose of decoction and MTX group could decrease mRNA level, and the effect of middle-dose is the best; Methotrexate group, the small-dose group and large-dose group of VEGF mRNA expression level difference is not much. Between Chinese traditional medicine group, middle-dose group had the best effect to VEGF mRNA level.CONCLUSION:By the analysis of experimental results, in the treatment of CIA rat, the curative effect of nourish kidney dispel cold cure Wangbi decoction was proofed nourish kidney dispel cold cure Wangbi can decrease arthritis index score; Each dose group of nourish kidney dispel cold cure Wangbi decoction could improve BMP-2expression of the bone tissue and the synovial organization,and decrease VEGF expression of the bone tissue and the synovial organization; The reason of the curative effect of rheumatoid arthritis may be the reduction of VEGF expression of tissue and synovial organization,led to reducing production of blood vessels;and improved BMP-2expression of tissue and synovial organization, and improved Bone Tissue Repair, promoted injuried bone healing.Disease development has individual differences, different dose may be have advantage in the treatment and prevention, needing further research and innovation; The correct dose of medication can enhance the clinical curative effect, also can reduce the unnecessary side effects; From the view point of resource allocation analysis. If clinical treatment use reasonable dose can save Drug resource.nourish kidney dispel cold cure Wangbi decoction may be used for a long time in clinical.Combined with clinical curative effect,The decoction should be researched further.

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