Dissertation > Excellent graduate degree dissertation topics show

Enhancement Effect of IFN-γ on the Phagocytosis,Killing Activity of RAW264.7Macrophages Against Trichosporon Asahii

Author: NieJianWei
Tutor: YangRongZuo
School: Anhui Medical University,
Course: Dermatology and Venereology
Keywords: Trichosporon asahii macrophage IFN–γ NO/O2-
CLC: R392
Type: Master's thesis
Year: 2012
Downloads: 57
Quote: 0
Read: Download Dissertation


Objective1. To determine the effect of interferon-gamma(IFN–γ) on the phagocytosis and killingactivity of RAW264.7murine macrophage cell line against T.asahii.2.To investigate the effect of IFN-γ on the killing mechanism of RAW264.7macrophage against Trichosporon asahii(T.asahii)in vitro.Methods1. RAW264.7cells incubated with various concentrations of recombinant murineIFN–γ for18h, then incubated with T.asahii for45min or4h. The phagocytosis activityof RAW264.7cells against T.asahii were observed using an inverted microscope afterstained with Wright’s Giemsa stain45min later. The killing activity was assessed bycounting the number of colony forming units (CFU)4h later.2. The various concentrations(10U/ml、100U/ml、1000U/ml)of IFN–γ with RAW264.7were incubated, and then cultured with T.asahii to assess the killing activity bycounting the number of colony forming units per milliliter (CFU/ml)of T. asahii.Thencalculate the T.asahii growth inhibition rate, and measure Nitrite ion(NO2-)in thesupernatant. Each well added by the above different terminal concentrations of IFN–γincubated with RAW264.7to determine the superoxide anion (O2-) in the supernatant. Results1. IFN-γ enhanced the phagocytosis and killing activity of RAW264.7cells againstT.asahii in a concentration-dependent manner ranging from10-1000U/ml (P <0.01).2. With the concentrations (10U/ml、100U/ml and1000U/ml) of IFN–γ, the growthinhibition rates of T.asahii were25.21%、41.95%、55.83%respectively.There wassignificant difference between each group (P<0.01).In the supernatant theconcentrations of Nitrite ion were89.16±3.32、108.07±3.81、126.44±3.52respectively.There were significant difference (P<0.01) compared to the blank controlgroup(74.47±1.48). With the concentrations of IFN–γ rising (10,100,1000U/mlrespectively), the OD value of the O2-measured in the supernatant were increasing(0.317±0.006、0.424±0.006、0.518±0.006respectively).And the blank control groupwas0.222±0.008, there was significant difference between each group(P<0.01).Conclusions1. IFN-γ can enhance the effect of the phagocytosis and killing activity of RAW264.7cells against T.asahii.2.With the concentrations of IFN-γ rising,the growth inhibition rates of T.asahii areincreasing. IFN-γ plays a role in killing effect of T. asahii in vitro by enhancingmacrophage producing nitrogen monoxidum(NO)and O2-.

Related Dissertations

  1. Effect of High Glucose on Chemokine Ligand 16 (CXCL16) Expression and Secretion in THP-1 Macrophages and Intervention of Aspirin in the Activity,R587.2
  2. Effects of Vitamin A and Montelukast on Airway Inflammatory Cells and Th1/Th2 Balance in Mouse Model of Asthma,R725.6
  3. Exploreing the Mechanism of Xieshifeiaifang Ii on Treating Lung Cancer with Pleura Effusion,R734.2
  4. The Effects of Prostaglantiain E1 to the Phenotype and Functional Activity of Macrophage Which in Mouse Uterus,R363
  5. Diagnosis Value of Cytokine Levels and TB-Ab in Serum in Children Tuberculosis and Latent Tuberculosis Infection,R725.1
  6. The Oxygen Reduction Activity of the Pt-Based Catalyst: First Principles Study,TM911.4
  7. The Expression and Significance of Serum MIP-1β and MCP-1in Patients with Rheumatoid Athritis,R593.22
  8. The Significance of Triggering Receptor Expressed on Myeloid Cells-1(TREM-1) in Neonatal Rats Septicemia with E.coli,R363
  9. Effects of Lymphocytes-Derived Catecholamines on the Differentiation of T Helper Cells,R392
  10. Effect of O2 Flow Rate and Low-frequency Power on Etching Characteristics of SiCOH Low-k Films,TB383.2
  11. Risk Factors of Stroke-associated Infection and Its Correlation with Stress Response and Cell Immunity,R743.3
  12. High-throughput screening in vivo inhibition of macrophage migration in zebrafish Chinese medicine,R285.5
  13. Study of Dendriti Cell Treatment Combined with Chemotherapy for Late Gastric Cancer,R735.2
  14. Effect of Hydrogen Peroxide and X-ray on Calcium Oscillation in Airway Epithelial Cells and Pulmonary Macrophages,R563.9
  15. Blocking TNFR Signaling Reduces the Recruitment and Migration of MDSC to Tumor Tissue,R730.3
  16. Phenotypic and Functional Study of NK and γδT Cells in Malignant Pleural Effusion,R730.4
  17. The Role of Interleukin-1 and Interferon-γ in the Pathogenesis of Nasal Polyps,R765
  18. Investigation of Infection and Role of Mycoplasma in Infertility,R711.6
  19. Study on the Effect of KG Ointment for the Treatment of Acute Eczema and the Influence of KG Ointment on the Expression of IFN-γ、IL-4、TNF-α and ICAM-1,R758.23
  20. Reseach of the Relationship between Shedding Virus of Subclinical Genital Herpes Patients and Organism Immune State,R752.1
  21. IFN-α2b Reversed the Resistence of Apoptosis by Fas/FasL Pathway Induced with IFN-γ in MCF-7 Breast Cancer Cells,R737.9

CLC: > Medicine, health > Basic Medical > Medical Immunology
© 2012 www.DissertationTopic.Net  Mobile