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96 Points Real-time PCR Detection System Development

Author: WangWeiPing
Tutor: TangYiPing；WuZhiHe
School: Zhejiang University of Technology
Course: Computer technology
Keywords: polymerase chain reaction rapid heating and cooling signal detection ct value standard curve quantitative PCR
CLC: R197.39
Type: Master's thesis
Year: 2011
Downloads: 72
Quote: 0
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Abstract


Real-time fluorescence quantitative PCR detection system is the current clinical nucleic acid testing, molecular diagnostics and life science research of key equipment, now widely used in biological and medical research in various fields. Real-time fluorescence quantitative PCR detection system research and development, to enhance and improve our country in the current international advanced biological and medical research equipment, in particular the Institute of molecular genetics to improve the equipment necessary for product research and development relative backwardness, participate in international competition of new products has great practical significance.This article describes the real-time fluorescence quantitative PCR detection system to achieve the basic principles of Hangzhou Bioer Technology Co., Ltd. on the development of the international advanced level of LineGene9600 real-time PCR detection system, composition and thermal cycling control systems, data analysis software, DNA nucleic acid Quantitative analysis software such as the principle of technology and related content. Its fast heating and cooling, temperature control and precision hardware system designed to maintain the principle of method and data processing software of introduction. Scan the bottom of the new structure through the use of patented technology for PCR thermal cycling system multi-agent detection, PC machines with a variety of detector means of communication, nucleic acid analysis of quantitative molecular diagnostic software development principles, structure, implement components of nucleic acids automatic analysis and processing. Also used in the current mainstream products industry characteristics and development of a more comprehensive analysis summary, real-time quantitative PCR instruments gave the advantages and disadvantages and future development of a more comprehensive analysis and discussion.In this article, we studied the existing mainstream real-time fluorescence quantitative PCR detection system and LineGene9600 project development of key technologies and the use of newly developed software system, successfully completed the product development projects, and into the international market. We got the company’s scientific and technological progress awards. Although fluorescence quantitative PCR detection system products have reached very advanced stages of the level, but with a variety of new technologies, new technologies continue to emerge, we must continue to develop improved, more advanced research and development of nucleic acid analysis equipment in the market competition.

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CLC: > Medicine, health > Preventive Medicine,Health > Health care organizations and career ( Health Management ) > Health care systems and institutions > Hospitals, general hospitals > Medical Devices \u0026 Equipment
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