Dissertation > Excellent graduate degree dissertation topics show

The Identificationand Rapdanalysis of Wheat Root Lesion Pathogens in Henan Province

Author: LiGuangShuai
Tutor: WangZhenYue
School: Henan Agricultural University
Course: Plant Pathology
Keywords: The wheat root nematodes Morphological identification Molecular biology identification RAPD analysis
CLC: S435.121
Type: Master's thesis
Year: 2011
Downloads: 8
Quote: 0
Read: Download Dissertation


Wheat root lesion has caused serious economic loss overseas. In 2006, a new disease in wheat root, root lesion nematode, was discovered in the science and technology park of Henan Agricultural University. It was the first report of pratylenchus infecting wheat. The results of field survey in Zhengzhou in 2007 showed that wheat yeild was reduced by 13.58%~15.46% after wheat root lesion nematode infection.In this study wheat root lesion nematode was identified in use of morphology analysis combined with molecular biology method. The results showed that the pathogen causing root lesion disease in wheat was Pratylenchus agilis. Previous study found Pratylenchus agilis infecting crop except wheat, and our study demonstrated that wheat is a newly host for Pratylenchus agilis.Preliminary investigations of the wheat root nematode were conducted in 14 areas of Henan province with 45 sample points. .The results showed that wheat root nematode can be extracted in 9 regions with 35 sample points except Zhumadian(south of Zhengzhou),Zhoukou,Zhongmou(east of Zhengzhou), and it distributed widespreadly and nonuniformly in Henan.We selected 23 typical samples from 35 samples to conduct the morphology and molecular biology analysis. It was found that these samples belonged to 2 different pratylenchus, of which 15 samples were named as Praylenchus agilis and 8 samples as Praylenchus loosi.RAPD technique was used to analyze the genetic diversity of 13 samples from 9 representative areas which were Zhengzhou, Xinxiang, Jiaozuo, Anyang,etc.This study screened 10 optimal polymorphism primers from 100 random primers. 85 RAPD markers were amplified in total, of which 81 markers were polymorphisms markers.The results showed that root lesion nematode was genetically diversified in Henan. NTSYS software was applied for cluster analysis of nematodes in this study. The result indicated that the pathogenic nematodes were divided into two branches and the rangeability of interspecific genetic similarity coefficient was 0.51~0.86. The wheat root lesion nematodes from same area were closely related and gathered together in the phylogenetic tree. Genetic diversity results were identical to that of morphology and molecular biology analysis further confirming the results of the other two identification method.

Related Dissertations

  1. Distribution and Identification of Cereal Cyst Nematode on Wheat in Jiangsu Province and Their Chemical Control,S435.121
  2. Physiological and Biochemical Characteristics and RAPD Analysis of the Aging Soybean Seed,S565.1
  3. Peach fruit moth and fungi pathogenic role of extracellular enzymes,S476.1
  4. Establishment of Breeding and Mating Technology of Xinjiao10 of Capsicum,S641.3
  5. Studies on the Grape Fruit Rot in Shihezi Region,S436.631.1
  6. The Study on the Flavour and Debittering of Porcine Bone Extract Hydrolysate and the Identification of Microbes,TS251.94
  7. Occurrence of Root-knot Nematodes on Vegetables in Hunan and Screening of Tomato Varieties with Resistance to Meloidogyne Incognita,S436.3
  8. The Morphological and ISSR Molecular Markers Identification about Several China Orchid,S682.31
  9. Physiological Race Identification and Diversity Analysis of Powdery Mildew (Podosphaera Xanthii) on Cucurbitaceae in Heilongjiang Province,S436.42
  10. Studies on Tissue Culture Andmolecular Genetic Diversity of Wild Cymbidium in Qilnnling,S682.31
  11. Pathogen Diversity of Sooty Blotch and Flyspeck Associated from Three Provinces in South China,S432.4
  12. Genetic Diversity of Hainan Arecanut and Yellow Leaf Disease of Arecanut Relations,S763.7
  13. Biodiversity Research of the Genus Trichoderma from Yunnan Province: Data from Morphology and ITS Sequence,S476
  14. Study on Population Genetic Structure of Blumeria Graminis f.sp.tritici in Sichuan,S435.121
  15. Isolation, Purification and Identification of Sulfate Reducing Bacteria and Achromatium,X703
  16. The Biology Effect Study of Medicago Sativa L. Carried by "Shijian 8" Recoverable Satellite,S541.9
  17. Based on SSR, ISSR and RAPD on the genetic diversity of Phytophthora sojae research,S435.651
  18. Northeast medicinal plant rhizosphere soil fungal diversity studies,S154.3
  19. Construction and Mutant Screening of EMS Induced Wheat Mutant Library,S512.1
  20. Enhanced UV-B radiation on wheat genomic DNA of,S512.1

CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Crop pests and diseases and their prevention > Cereal crop pests and diseases > Wheat pests and diseases > Disease
© 2012 www.DissertationTopic.Net  Mobile