Dissertation > Excellent graduate degree dissertation topics show

Protective Effect of Edaravone on Hepatic Injury with Severe Acute Pancreatitis in Rats

Author: ZhangZhiJian
Tutor: LuoYuHong
School: Jinan University
Course: Surgery
Keywords: Severe acute pancreatitis hepatic injury oxygen free radical edaravone superoxide dismutase malondialdehyde
CLC: R657.51
Type: Master's thesis
Year: 2013
Downloads: 2
Quote: 0
Read: Download Dissertation

Abstract


ObjectivesThe aim of this study is to investigate the protective effect and mechanism of edaravoneinjection on hepatic injury in rats with severe acute pancreatitis by animal experiments, and toobserve the changes in serological markers like AMY, AST, ALT, TNF-, IL-6, SOD and MDAduring the courses of severe acute pancreatitis.Methods72Male Sprague-Dawley (SD) rats were randomly divided into3groups with24rats in eachgroup-Sham operation group (SO Group); Severe acute pancreatitis model group (SAP Group);Edaravone group (EDA Group).1ml/kg of4%sodium taurocholate solution was injected byretrograde puncture of pancreatic duct through duodenum in order to set up SAP model.3mg/kgof edaravone was injected intraperitoneally in EDA Group. Same volume of normal saline wasinjected intraperitoneally in SAP Group. Open incision in abdomen was made after anesthesia inSO Group, then turning the duodenum and pancreas, and abdominal injection of an equal volumeof saline. Normal saline (30ml/kg) was injected subcutaneously in all groups to compensate thewater loss during modeling. Blood samples and specimens from pancreas and liver were takenfor tests at4,8,12hours (n=8in each time point) after modeling. Test indices including serumamylase (AMY), serum alanine aminotransferase (ALT), serum aspartate aminotransferase(AST), serum tumor necrosis factor-(TNF-), interleukin-6(IL-6), serum superoxidesuperoxide dismutase (SOD), serum malondialdehyde (MDA), and pathological score ofpancreas and liver tissues. All the data collected was analyzed by SPSS13.0for windowsstatistical software.ResultsSerum markers and the pancreas, hepatic pathology scores were no significant changes in SOgroup, the3groups at each time point compared to each other were not statistically different (P>0.05). Serum AMY, ALT, AST, TNF-, IL-6and MDA levels of SAP Group in the3time pointswere higher than that in SO Group. SOD was lower compared with SO Group (P<0.05).Pancreas and liver pathological score was higher than that in SO Group (P<0.05). Indices inEDA Group and SAP group improved with the time at the3time points (P<0.05). Pancreas and liver pathological score was lower than that in SAP Group (P<0.05).Conclusions1.4%sodium taurocholate injection by retrograde puncture of pancreatic duct throughduodenum is an easy way to set up SAP and hepatic injury model. It is simple, reliable,reproducible, and an ideal SAP modeling.2. TNF-, IL-6, MDA and SOD is an importantparameter of inflammatory reactions in SAP and it is an important factor of SAP with hepaticinjury.3. Edaravone can lower the degree of hepatic injury in SAP. Its mechanism may be relatedto the inhibition of serum inflammatory mediators such as TNF-, IL-6generation, andclearance of excess free radicals in the body and reducing the loss of liver cells.

Related Dissertations

  1. The Research of Supramolecular Complexes of Edaravone and Cyclodextrins,R943
  2. Study on Extraction and Properties of Superoxide Dismutase from Goose Blood,TS254.9
  3. The Changes of Intestinal Barrier Functional in Rats with SAP and the Effection of QingYi Ⅱ,R657.51
  4. Shamisen Medicinal and Edible Herbs on glucose and lipid metabolism in the metabolic syndrome and antioxidant effects,R285.5
  5. Berberine on serum levels of superoxide dismutase and malondialdehyde levels in,R743.33
  6. Bone marrow mesenchymal stem cells in rats with severe acute pancreatitis CD4 ~ CD25 ~ regulatory T cells influence and Integrative Medicine,R576
  7. Edaravone promote functional recovery of sciatic nerve injury in early experimental study,R965
  8. Edaravone , lamivudine , pharmaceutical intermediates research and development,TQ463.5
  9. Rhubarb , magnesium sulfate treatment of severe acute pancreatitis comparative study of the clinical efficacy,R576
  10. Diagnostic Value of Serum SP-A and SP-D of Severe Acute Pancreatitis(SAP)-induced Acute Lung Injury(ALI),R563.8
  11. The Effection of the HMGB1 on the Pathways of Pancreatic Acini Cell Death in Rat with SAP,R576
  12. The Changes of Oxidative Stress Index in the Serum of Mice Exposured to Intermittent Hypoxia and the Therapeutical Effect of NAC,R766.43
  13. The Effect of Exogenous Melatonin to Intrauterine LPS Exposure on Oxidative Damage and the Expression of Tnf-αin Fetal Rat Brain,R363
  14. An Experimental Study on the Protective Effect of Edaravone on Liver Graft against in Rat OLT Model,R657.3
  15. Clinical Analysis of 81 Cases of SAP Treated by Traditional Chinese Medicine and LipoPGE1,R576
  16. Expression and Immunoprotective Analysis of Superoxide Dismutase from Vibrio Harveyi on Turbot (Scophthalmus Maximus),S941.4
  17. Study of Silphium Perfoliatum L-SOD,S548
  18. Studying on Environmrntal Biomarkers Induced by PAEs,X171.5
  19. Study of Determination of Activity for Antioxidant Enzyme System of Tomato Leaf and Plant Based on Hyperspectral Imaging Technology,S641.2
  20. The Anti-Aging Function of Curcumin and Essential Fatty Acids,R285.5
  21. The Clinical Value of and Curative Effect of Therapeutic ERCP in Serious Pancreatic Diseases,R657.51

CLC: > Medicine, health > Surgery > Of surgery > Abdominal surgery > Pancreas > Pancreatitis
© 2012 www.DissertationTopic.Net  Mobile