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Study on the Expression and Relationship of Tumstatin and VEGF in Renal Cell Carcinoma

Author: JieHai
Tutor: WangJianMin
School: Tianjin Medical University
Course: Surgery
Keywords: Renal cell carcinoma Tumstatin VEGF Angiogenesis
CLC: R737.11
Type: Master's thesis
Year: 2013
Downloads: 1
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Abstract


Background and Objective:Renal cell carcinama is a malignant tumor originating from renal tubular epithelial cells, accounts for about2~3%of adult malignant tumor, only after bladder cancer ranks the second in the adult urinary and reproductive system tumor morbidity, and its incidence has continuously increased in recent years. Over the years, application of molecular immunology, gene therapy and molecular antiangiogenic therapy targeting, has satisfying effect for advanced or metastatic renal cell carcinoma. Study on angiogenesis and inhibiting factor for renal cell carcinoma in recent years gradually, angiogenesis is born in mature vascular network of new blood vessels from existing in, on the growth and metastasis of solid tumors plays a key role. Renal cell carcinoma with genetic biology characteristics and heterogeneity of special, more prone to metastasis, are not sensitive to radiotherapy and chemotherapy, is a highly vascularized malignant solid tumor. Study confirmed that the vascular endothelial growth factor expression in vitro and in vivo (VEGF), a potent angiogenic factors are widely studied, play an important role on promoting the process of tumor angiogenesis, but also promote the invasion and metastasis of the tumor; tumstatin can specifically inhibit protein synthesis of tumor vascular endothelial cells, induces the apoptosis of endothelial cells, the neovascularization synthesis is inhibited, thereby inhibiting the tumor growth, invasion and metastasis. The purpose of this experiment is to study the inhibition and promotion regulation of angiogenesis factor, including correlation effects of Tumstatin and VEGF in renal cell carcinoma patients and between, and provide a theoretical basis for the biological treatment of renal cell carcinoma.Materials and methods:A total of50consecutive patients from department of urology, second hospital of Tianjin medical university, Tianjin institute of urology (28males and22females) with RCC undergoing nephrectomy and information complete, with accession from May2008through February2010, were included in this study. The median age was60.2years with a range of30-78years. WHO grade:I grade was in14cases, Ⅱ grade was in26cases, Ⅲ grade was in10cases. Robson clinical stage: Ⅰ stage was in14cases, Ⅱ stage was in15cases, Ⅲ stage was in12cases, Ⅳ stage was in9cases. Twenty-eight normal tissue of>2cm domain beside tumor were recruited as normal controls. All sections stained by the same experienced pathologists in the same conditions by Olympus optical microscope with double blind reading. Tumstain, VEGF protein positive expression in tumor cell cytoplasm brown-yellow granules. As the expression level of accurate judgement of protein and reduce false positive rate. The score criteria apply method of liang zhong Xu, randomly selected10high-power fields were recorded in1000renal cell carcinoma, were scored positive coefficient calculated according to the percentage of coloring degree of cytoplasm and colored cells. SPSS13.0software application was used for all analysis and statistical significance was defined as P values less than0.05.Results:(1) The expression ratios of Tumstatin in normal kidney tissue and in renal cell carcinoma were78.6%and42%respectively, showing significant difference (χ2=11.790,P<0.01). The Tumstatin expression in50cases of carcinoma was associated with clinical stages (P<0.01), while no significant difference was detected in sex, age, the size of tumor and histologic grade.(2) The expression ratios of VEGF in normal kidney tissue and in renal cell carcinoma were32.1%and68%respectively, showing significant difference (χ2=19.588, P<0.01). The VEGF expression in50cases of carcinoma was associated with clinical stages (P<0.01), while no significant difference was detected in sex, age, the size of tumor and histologic grade.(3) The relationship between the expression of Tumstatin, VEGF in renal cell carcinoma show that Tumstatin expression was negatively correlated with VEGF (P<0.01) Conclusion:(1) Tumstatin and VEGF were expressed in normal kidney tissue and in renal cell carcinoma, there is a significantly correlation between them in renal carcinoma and clinical staging.(2) The low expression of Tumstatin in renal cell carcinoma tissue, high expression in normal kidney tissue, high expression of VEGF in renal cell carcinoma, in low expression in normal tissues, Tumstatin expression was negatively correlated with VEGF, confirmed that Tumstatin and VEGF are important factors of renal cell carcinoma angiogenesis.(3) Through the experiment to deepen the understanding of renal cell carcinoma angiogenesis regulation mechanism, Tumstatin, VEGF may be important targets for antiangiogenesis therapy in renal cell carcinoma.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Urinary tumors > Kidney,renal pelvis tumor
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