Dissertation > Excellent graduate degree dissertation topics show

Changes of TIM-3and GAL-9Expression at the Brain of Rats with Experimental Autoimmune Encephalomyelitis

Author: DengZuo
Tutor: TanLiMing
School: Central South University
Course: Clinical
Keywords: Experimental autoimmune encephalomyelitis (EAE) Multiple sclerosis (MS) TIM-3 GAL-9 IL-17
CLC: R744.3
Type: Master's thesis
Year: 2012
Downloads: 43
Quote: 0
Read: Download Dissertation

Abstract


ObjectiveWe investigate the changes of TIM-3and its ligand GAL-9mRNA at the brains of rats with experimental autoimmune encephalomyelitis, and detect the changes of IL-17mRNA in different phase of EAE. Explore the mechanism of TIM-3/GAL-9pathway in the happening and development of EAE.MethodsForty-eight Wistar rats were randomly divided into two groups:each twenty-four rats in EAE group and CFA group, then the two group were randomly divided into four subgroups:at the onset, peak, remission and recover. The animal model was established in female Wistar rats by immunizing rats with guinea pig spinal cord homogenate (GPSCH), complete freund’s adjuvant (CFA). The CFA group use NS instead of GPSCH. The severity of EAE was scored on a scale0-5according to the signs and symptoms. Histological examinations were performed on the sections of brain with the aid of hematoxylin-eosin and Luxol Fast Blue myelin staining. The expressions of TIM-3and GAL-9mRNA in four subgroups’brain tissue were detected by RT-PCR. Experimental results were analyzed with SPSS17.0.Results 1. The CFA group had no ill. The morbility of EAE group is83.33%.83.33%rats in EAE group were developed a progressive paralysis, the clinical symptoms of EAE occurred about8th-10th days after immunization were induced. The peak of clinical symptoms took place on the12th-14th days, and got a remission at15th-17th days.2. The CNS tissue’s hematoxylin-eosin(H-E) staining histopathology results of EAE group’s rats indicate that CNS already have inflammation focus at the onset, the extent and amount of the inflammation focus increased on the12th and14th day, then decreased on the15th-17th day, reach normal level on the18th-22nd day.3. Comparing to the CFA group, The TIM-3mRNA in EAE were increased as the disease got aggravating (P<0.05), and peaked at the remission, then decreased at the recover phase. The expression of GAL-9mRNA of brains in EAE groups increase at early (P<0.05), followed an increase in the peak phase. The expression of IL-17mRNA of brains in EAE groups were increased on the onset, peaked on the12th day (P<0.05), the expression level decreased on15th day, but still higher than CFA group. The expression of TIM-3and GAL-9mRNA was positive correlated with the expression of IL-17mRNA and the correlation coefficients were0.699and0.897.Conclusion1. GAL-9and TIM-3mRNA increased in EAE rats, which suggest that TIM-3and GAL-9play an important role in the central nervous system.2. The expression of TIM-3and GAL-9mRNA was positive correlated with the expression of IL-17mRNA in EAE rats, suggesting the TIM-3/GAL-9pathway can regulates IL-17.

Related Dissertations

  1. Effects of Blockade of IL-17 in Already Established Asthma of BALB/c Mice,R562.25
  2. A Stuty on the Diversification of Interleukin-17 of Asthma Mouse and the Affection of the Treament of Glucocorticoids,R562.25
  3. Asthma serum IL-17 and IgE changes and Correlation Analysis,R562.25
  4. Detection and Quantification of Th17 Cells and IL-17 in the Peripheral Blood and Tumor Tissue in Patients with Non-small Cell Lung Cancer,R734.2
  5. The Assay and Clinical Significance of TC17 Cells and Its Main Effect Cytokine IL-17 in the Peripheral Blood of NSCLC Patients,R734.2
  6. Function of Th17 Cells and Change of CD4~+CD25~+T Regulatory Cells in Children with Henoch-Schonlein Purpura in Acute,R725.5
  7. The Expression and Significance of Tim-3-Gal-9 Pathway in Murine Rsa Models,R714.21
  8. Study on the Role of Tim-3 in Spontaneous Abortion and Immune Tolerance,R714.21
  9. Study on the Expression Level of Toll-like Receptor-4 and Interleukin-17 in a Mouse Model of Systemic Candidiasis,R519
  10. The Expression of Tim-3 on Peripheral Blood Mononuclear Cells and Pathological Significance of Tim-3 Expressing in Rheumatoid Arthritis Patient with High Disease Activity,R593.22
  11. Continuous Variable Quantum Cryptography Communication Experiment.,O413
  12. The Dynamic Expression of Induced IL-17 and IL-23 from Mice Infected with Schistosoma Japonicum,R392
  13. The Relationship between Expression of Interleukin-17 and Ventricular Arrhythmia in Rats with Chronic Heart Failure,R541.7
  14. Experimental Study of Th17 Related Factors of Animal Models Infected with Mycoplasma Suis,S858.28
  15. The Research on Polymorphism of the Tim-3 Gene in the Uygur and Han Allergic Rhinitis Xinjiang,R765.21
  16. Study on the Polymorphisms of Childhood Asthma Gene TIM Family and Relative Factors,R725.6
  17. Effects and Mechanism of Arsenic Trioxide on Th17/IL-17 Axis in a Mouse Model of Asthma,R562.25
  18. The Condition of Equilibrium of Th17 Cells、Treg Cells and IL-17 in the HIV or AIDS as Well as Their Influence in HAART for One Year,R512.91
  19. The Roles and Mechanisms of Methotrexate or Simvastatin on Th17/IL-17 in Peripheral Blood Mononuclear Cells from Rheumatoid Arthritis in Vitro Study,R593.22
  20. The Clinical Observation in Shen Kang Jiang Suan Particles to Gouty Nephorpathy of Influence to GAL/NAG and XOD,R259
  21. Submission and Subversion,I712

CLC: > Medicine, health > Neurology and psychiatry > Neurology > Spinal cord disease > Myelitis
© 2012 www.DissertationTopic.Net  Mobile