Dissertation > Excellent graduate degree dissertation topics show

Mechanism Investigation of miR-26a’s Regulation of Anoikis in Human Hepatocellular Carcinoma

Author: ZhangXiang
Tutor: YangAnGang; ZhangRui
School: Fourth Military Medical University
Course: Biology
Keywords: HCC miR-26a Integrin Anoikis Chimeric microRNA
CLC: R735.7
Type: Master's thesis
Year: 2012
Downloads: 61
Quote: 0
Read: Download Dissertation

Abstract


The role of microRNAs (miRNAs) in human tumorigenesis has beendemonstrated by gene profiling and functional studies. In hepatocellularcarcinoma (HCC), consistently deregulated miRNAs were identified. Mostimportantly, functional and molecular studies uncovered mechanisms that linkedderegulated miRNAs to cancer-associated pathways. In fact, a large number ofstudies have proved that miRNAs themselves could be successfully used for invivo modulation of miRNA actions. The demonstration has shown significantpotentials in molecularly targeted therapy.miR-26a family contains two members, including miR-26a-1andmiR-26a-2. It was reported that, the down-regulation of miR-26was associatedwith not only development of HCC, but also shorter overall survival. Besides,miR-26a was able to inhibit cell proliferation in HCC by targeting CCND2&CCNE2. However, it was report that, about ninety percent of patients with HCCdied from metastasis. So it is very important to identify the relationship between miR-26a and metastasis.In our research, at the beginning, we proved that overexpression ofmiR-26a could promote the anoikis of HCC, in vitro and in vivo. Besides, it wasconfirmed that ITGA5was a target gene of miR-26a, and the ITGA5-lost HCChad similar phenotype with miR-26a-gained HCC. At last but not at least, inrescuing assay, when miR-26a-gained HCC overexpressed ITGA5again, theiranoikis was inhibited. To sum up, we found a new relationship in HCC.MiR-26a promote anoikis in HCC by targeting ITGA5.Based on review and our data, we can make a conclusion that miR-101andmiR-26a are both important tumour suppressor genes. Their coalition may makemore effort in HCC therapy. However, if two kinds of microRNAs are deliveredin the same time, the quantity of each kind of microRNAs is lower, which mustdecrease the effect of each kind of microRNA. So, it is a new problem that howto increase the effect of every single microRNA.In our research, we constructed a kind of chimeric microRNA (CM), whichcontains two seed-sequence-included halves of mature microRNAs. Then, CM’shigh efficiency was confirmed as follows, first, CM’s each seed-sequence-included half had similar function with corresponding nature microRNA.Second, CM’s two seed-sequence-included halves had similar function with twonature microRNAs.

Related Dissertations

  1. Treatment effect and its mechanism of lactose derivatives Gu- 4 in endotoxin shock in mice,R459.7
  2. Preliminary Study of Molecular Epidemiology on Hepatitis B among Certain Regions in Anhui Province,R512.62
  3. Implanted in mouse endometrium during early morphological and Galectin-3, expression of integrin α4,R321
  4. The Anti-tumor Effects of Lupeol in Hepatocellular Carcinoma,R735.7
  5. The Expression of GPC3、 AFP、 HCG and CD34 in Hepatocellular Carcinoma,R735.7
  6. Studies on the Tumor Targeting of RGD Peptide Modified Stearic Acid-g-Chitosan Oligosaccharide Polymeric Micelles,R943
  7. The Role of Integrin β1 Up-regulated in Resistance of Non-small Cell Lung Cancer to EGFR TKI,R734.2
  8. Study of the Expression and Function of TC21/R-Ras2 in Hepatocellular Carcinoma,R735.7
  9. Whole Exome Capture Sequencing Identifies Candidate Genes Related to HCC,R735.7
  10. A Pro-inflammatory Mediator Leukotriene D4 Involved in the Progression of Chronic Hepatitis B and Hepatocellular Carcinoma,R512.62
  11. Wisp-1: A Novel Mediator Linking HBV and Hepatocellular Carcinoma,R512.62
  12. Constructing of Recombinant Plasmids Containing shRNA Targeting Notch1 and the Effects on the Biological Behavior of L02/HBx Cell,R735.7
  13. Investigation on New Topology and Control Strategy of PMSG-based Wind Power System,TM315
  14. Inhibition of CD24 Expression to Induce Anoikis in Ovarian Cancer Cells,R737.31
  15. A Preliminary Investigation of the Expression of SK4 in Hepatocellular Carcinoma,R735.7
  16. The Impact of HBV on Oval Cells and Its Effects on the Expression of P8,R735.7
  17. Expression and Clinical Significance of SK4 in Hepatocellular Carcinoma, Breast Carcinoma and Thyroid Carcinoma,R730.4
  18. Influence of Synuclein-γ on the Effectiveness of Antimicrotubule Drugs in Human Hepatoma Cells and the Mechanism,R735.7
  19. Involvement of NF-κB Pathway in Multidrug Resistance Induced by HBx in Hepatoma Cell Line,R735.7
  20. Screening and Identification of Metastasis-associated MicroRNAs in Human Hepatocellular Carcinoma,R735.7
  21. Immunosuppressant Tacrolimus on the mechanism of invasion and metastasis of hepatocellular carcinoma,R735.7

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
© 2012 www.DissertationTopic.Net  Mobile