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Papaya papaya ringspot virus disease is the first major viral diseases, the worldwide cause greater harm , there is no very effective chemicals to control PRSV disease , but to take to cultivation measures based comprehensive prevention and control measure. The rapid development of plant genetic engineering for plant disease resistance provides a new and effective methods, especially in recent years, the rapid development of RNAi research in the field , the use of inverted repeat expression vectors for plant genetic transformation of plants to become one of the major anti- virus research hotspot. In this study, embryoid papaya , cucumber cotyledon as conversion material , the use of Agrobacterium- mediated method inverted repeat expression vector pHellsgate-CIIR, pHellsgate-NIbIR imported into the acceptor material , it is desirable to obtain transgenic plants . While taking advantage of protoplast transient expression system has been initially studied inverted repeat of the target gene expression vector silencing effect . The main results are as follows: ( a ) on papaya hypocotyl , petioles, and papaya immature embryo callus induction of embryoids , only from immature embryo callus and somatic embryos obtained papaya , which embryo induced embryoid quantity, consuming short , 40d so that is induced from immature embryos embryoid induction was 20%. ( 2 ) cucumber cotyledon node as explants to establish a cucumber regeneration system containing the expression vector pHellsgate-CIIR, pHellsgate-NibIR cucumber cotyledon node Agrobacterium infection by antibiotic selection , has been transformed cucumber seedlings . ( 3 ) with PRSV virus particles and inverted repeat expression vector pHellsgate-CIIR co-transformed papaya protoplasts , 12,24,36,48 h were sampled by protoplast total RNA extraction , RT-PCR , and the results show that for only PRSV transformed protoplasts , 24h able to detect the virus began ci gene expression, and with time , ci gene expression gradually increased ; for PRSV and inverted repeat of the expression vector pHellsgate-CIIR papaya transformed protoplasts virus was not detected ci gene expression , the expression vector described repeat product pHellsgate-CIIR ci gene expression can be suppressed .
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