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Technology of Triticale Haploid Induced by Maize Pollen and Genetic of Haploid Induction Rate

Author: WangXueYu
Tutor: KongGuangChao
School: Shihezi University
Course: Crop Genetics and Breeding
Keywords: Triticale Corn pollen Haploid Inheritance
CLC: S512.4
Type: Master's thesis
Year: 2011
Downloads: 57
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Abstract

Haploid breeding is to accelerate the breeding process, a powerful means to improve the efficiency of breeding. Traditional breeding techniques are mostly used haploid pollen or anther culture means to obtain haploid plants, followed by chromosome doubling technology obtained through homozygous doubled stable monomer material. But anther or pollen culture process is more complicated and easy to produce asexual culture process variation, thus restricting its large-scale application. The use of corn pollen induced thereby doubled haploid wheat cultivation doubled haploid, because technology is simple and feasible, its application is gradually increased. This study was to explore the establishment of corn pollen induced haploid technology triticale, and their inheritance of haploid induction rate of study is designed to provide a convenient and triticale breeding efficient breeding techniques provide a theoretical basis. This selection of the different genotypes of spring triticale materials and 15 winter triticale materials were induced using four different methods, namely corn pollen induced synergistic treatment with chemical inducers (corn pollen induced with dimethyl sulfoxide 40m1 / L, colchicine 4g / L, inositol 100mg / L, indole acetic acid 20mg / L and 2,4-D 20mg / L combined stimulation), chemical inducing agent treatment alone (DMSO 40m1 / L, colchicine 4g / L, inositol 100mg / L, indole acetic acid 20mg / L and 2,4-D 20mg / L treatment), corn pollen induced combined with hormonal stimulation (corn pollen induced after 100mg / L 2,4-D and 30 m1 / L DMSO treatment) and pure hormone stimulation (only 100mg / L 2,4-D 30ml / L DMSO treatment), and the different haploid embryo rescue medium, different corn genotypes as well as different triticale triticale genotypes affect haploid embryo formation were analyzed, and 12 of embryo quite different spring triticale genotypes as materials, fully equipped with 6 × 6 diallel, their parents and F1 progeny of embryo heritability, genetic correlation, combining ability and heterosis of offspring were analyzed for the establishment of maize haploid induction triticale to explore technology and its genetic law laid the foundation. The main results are as follows: 1. Induction in the four test mode, its rate of haploid embryos obtained after pollination of corn to 100mg/L2 ,4-D and 30ml/LDMSO treated as the highest (6.65%), in corn pollination back via DMSO 40ml / L, colchicine 4g / L, inositol 100mg / L, indole acetic acid 20mg / L and 2,4-D 20mg / L, followed by co-processing effects ( 4.42%). Two different genotypes of winter triticale corn pollen induced by treatment with hormones can produce haploid triticale, but different triticale genotypes of embryo differences, which have the highest rate of embryo genotype caryopsis rate up to 39.80%, while the lowest rate was embryo genotype was 1.37%. In addition to the impact of triticale genotypes, the different types of maize pollen-induced differences in efficiency, which burst corn pollen induced the highest efficiency (4.71%), followed by normal corn (2.94%), sweet corn pollen-induced effect is the most Poor (1.76%). 3 induced the formation of haploid embryos improvement on B5 medium 1/2MS and embryo rescue, although the two media of different genotypes on the whole seedling rate between haploid significant difference was not detected but different triticale genotypes haploid embryo rescue efficiency differences still exist between the two media, showing haploid triticale genotypes of different types should be selected optimal rescue medium. 4 haploid induction triticale genetic effects analysis showed that the haploid inducing traits are in line with the additive - dominant genetic model, and most traits partial dominance, which was the rate of genetic embryo to additive gene effects-based, caryopsis rate of embryo and caryopsis emergence of genetic as well as by additive gene effects and dominance effects. Heterosis strong induction rate and a higher heritability parents, according to the level of combining ability of parents to choose the good of F1 hybridization experiments. This study established a corn pollen-induced haploid triticale breeding technology; initially clear corn pollen induced haploid triticale genetic law. The study will guide triticale haploid breeding technology provides a solid theoretical basis.

Technology of Triticale Haploid Induced by Maize Pollen and Genetic of Haploid Induction Rate

Abstract

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