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Molybdenum, antimony anti colorimetry, Salkowski colorimetric plate confrontation method study the six phosphorus solubilizing bacteria (Jm170, Jm92, Lx191, Lx22, Jx59, Lx81) and four nitrogen-fixing bacteria (W5 N4, G, S11 ) interaction effect, measured the response of each strain is a combination of environmental factors, dissolved phosphorus, plant growth hormone secretion (IAA), antagonize plant pathogenic fungi characteristics. Meanwhile, the use of pot experiment measuring single and composite inoculants growth of alfalfa (Medicago sativa L.) The results showed that: 1. The tested 6 dissolved phosphorus bacteria and 4 diazotrophs antagonistic phenomenon, mixed culture. Compared with strains cultured alone, dissolved phosphorus mixed culture of bacteria and diazotrophs wider adaptability to environmental factors such as temperature, pH and salinity. Compared to phosphorus-solubilizing bacteria and nitrogen-fixing bacteria cultured alone twenty-two mixed culture with strains, phosphate solubilization ability of most of the mixed system, which combination Jx59 G, the Lx22 G combination and Jm92 G combination phosphate solubilization were 306.26 mg / L, 291.43 mg / L and 235.22 mg / L, compared with the two strains were cultured alone and increase, respectively, an increase of 42.28%, 46.91%, 52.02%; the the minority mixed system dissolved phosphorus diminished capacity, which Lx191 W5 combination, Jx59 N4 combination Jm92 S11 combination than dissolved phosphorus strains cultured alone reduced by 44.88%, 32.52%, and 16.28%, respectively. 3. Dissolved phosphorus bacteria and nitrogen-fixing bacteria Polymicrobial hybrid combination, Jm92 Lx22 G combination of the highest amount of dissolved phosphorus, 330.42 mg / L, and of Jm92 G combination of and Lx22 G, combination, compared with an increase of 40.47% and 13.38%, Jm92 Lx22 S11 The combination of dissolved phosphorus content is higher, a decrease of 2.43% compared to 322.55 mg / L, with Jm92 Lx22 G combination. Most of phosphate solubilizing bacteria and nitrogen-fixing bacteria combination mixed culture liquid organic acid content was significantly higher than cultured alone; phosphate solubilizing bacteria cultured alone, the amount of dissolved phosphorus, pH and organic acids in the presence of significant correlation, but the mixed culture weak correlation between the amount of dissolved phosphorus, pH and organic acid content of the combined system. 5. Phosphorus-solubilizing bacteria and nitrogen-fixing bacteria cultured alone, most diazotrophs the G strains secretion of growth hormone ability the for 37.34μg/ml; phosphate solubilizing bacteria and nitrogen-fixing bacteria combination of two cultures, Lx22 G Jm92 N4 combination of the secretion of growth hormone up, respectively from as 36.71μg/ml and 33.19μg/ml Jm92 N4 in Lx191 N4 and Jx59 N4 combination compared with a combination of the two strains were cultured alone increased by 428.81%, 162.5% and 7.89% respectively. The Lx191 Lx22 G combination secretion of growth hormone the highest 41.15μg/ml. Phosphorus solubilizing bacteria and diazotrophs a mixed culture twenty-two 48 combinations, only nine combinations of chili Rhizoctonia solani, Rhizoctonia solani was inhibited watermelon Fusarium and cotton, Jm92 N4 the three pathogens highest inhibitory rate were 80.38%, 79.06% and 75.12%, respectively. 7 test tube slant culture method, distilled water Preservation Act, the dissolved phosphorus bacteria dissolved phosphorus decreased ability to vacuum freeze-drying method saved less, it is recommended that for a long time to save strains using this method. 8. Compared to a combination of treatment and control of single strains and mixed strains could increase the alfalfa plant height, root length, nodule number, the amount of aboveground and underground phytomass, compared with the single-strain inoculation treatment, mixed inoculation treatment growth promoting better Jm92 Lx22 S11 G deal with growth promoting effect best, aboveground biomass than the control increased by 121.21 percent, followed by Jm92 Lx22 G processing and Jm92 Lx22 N4 S11 treatment, aboveground biomass than the control increased by 69.24% and 60.15% 9. hybrid strains single strain can better promote alfalfa nitrogen, phosphorus absorption Jm92 Lx22 S11 G treatment best alfalfa nitrogen content and phosphorus content than the control significantly increased, followed by of Jm92 Lx22 G processing and Jm92 Lx22 N4 S11 processing The combination of these three is expected to become the alfalfa rhizosphere inoculant or bacterial fertilizer.
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