|
As a simple structure of the nano-material, the nano-silica having the characteristics of ease of synthesis and surface modification, not only been widely used in the industrial field, and also used for the diagnosis, imaging and drug loading and other biomedical field. The study shows that the nano-silica can induce apoptosis of normal cells, thereby exhibiting a certain degree of cytotoxicity. However, so far, about nano silica antitumor activity also less. Nanometer silica (SNP) in order to study the anti-tumor activity, the selection of the particle size is 7 nm, 20 nm and 50nm three SNP particles to normal human liver cell L-02 as a control study SNP on human hepatoma cell HepG2 cell toxicity. The research results show that the SNP anti-tumor activity of particle size, concentration effects and time effects, particles of 20nm strongest inhibitory activity of hepatoma cells, the 7nm followed, 50nm weakest. SNP concentration for 20nm 160μg/ml of HepG2 cell activity can be reduced to 18.5% after 72h. However, SNP activity of L-02 cells are smaller, 20nm and 7nm two particle concentration, respectively, 320 and 640μg/ml cell activity decreased to 81% and 73%. Compared with the L-02 cells, the SNP of 20nm easier to enter HepG2 cells, and located in the nucleus, and cell apoptosis. With the rise of the role of nano-silica concentration of caspase-3 activity increased, the increase in the expression level of p53, Bcl-2 and procaspase-9 expression decreased Bax expression is essentially the same. 7nm and 20nm SNP allows intracellular reactive oxygen species (ROS) levels rise, glutathione (GSH) levels, oxidative stress on cells. Based on the above results, we speculated, SNP lead HepG2 cells oxidative stress, apoptosis through mitochondrial pathway signaling pathways induced liver cancer. Also, further 20nm the SNP modified, adjusting the surface potential of the nanoparticles. Zeta potential of -22.1 ~ 4.16 mV 6 the SNP, pick SNP20 (-22.1 mV), SNP20a (-8.93 mV) SNP20b (-0.70 mV) and SNP20c (4.16 mV) four particles investigated their impact on the human hepatoma cell line HepG2 cytotoxicity. The results showed that the cytotoxicity of the four kinds of silica particles is slightly different, and found in the four kinds of particles the ability to induce apoptosis is also a slight difference in the flow cytometry experiments. It is speculated that the surface of the potential value of the nano-silica particles may be related to anti-hepatoma cell activity.
|