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Screen of Predictor Related to Trauma-induced Deep Vein Thrombosis and Influence on Thrombosis Formation by Inhabiting Complement Activity in Traumatic Rats

Author: DengJinJing
Tutor: BaiBo
School: Guangzhou Medical College
Course: Surgery
Keywords: Deep vein thrombosis Trauma Proteomics Serum Two-dimensional electrophoresis Differentially expressed proteins Cobra venom factor (cobra venom factor, CVF) Anti-complement activity Coagulation indicators
CLC: R641
Type: Master's thesis
Year: 2011
Downloads: 9
Quote: 0
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Abstract


Deep vein thrombosis in a rat trauma after serum proteomics preliminary research purposes using proteomics technology research and analysis of the physical trauma serum, serum thrombosis group and compare rat limb post-traumatic thrombosis group changes of the protein group, looking for early serum markers can predict deep vein thrombosis. Constructed experimental animal model of SD rat lower limb trauma, to collect blood samples purified serum proteins, two-dimensional electrophoresis using isoelectric focusing (IEF) to the first, SDS-PAGE was second to two-dimensional electrophoresis, using pattern analysis than software detection and mass spectrometry technology and bio-IT Identification of differentially expressed proteins. Results A total of 13 protein spots were successfully identified, mass spectrometry sequencing results showed that the 13 points corresponding to seven different proteins, 12 protein spots were up-regulated in thrombosis serum, a protein spots thrombosis group upregulation and complement factor B, retinol-binding protein, glutathione peroxidase 3 precursor, complement component 4a, alpha-2u globulin precursor, Chain A, Rat Transthyretin, complement component C3. Conclusion: The two-dimensional gel electrophoresis - mass spectrometry can be effectively separated and identification of serum proteins, thrombosis group relative to thrombosis serum different proteins, this technology is finding post-traumatic deep vein thrombosis related serum markers provides a methodological basis . Initial procoagulant role in the thrombosis group regulated expression of multiple components of the complement thrombosis plays. Second cobra venom factor consumption complement experimental study of the formation of the Traumatized deep vein thrombosis purpose of the use of cobra venom factor inhibits complement effect observed limb trauma rats were injected with cobra venom factor coagulation indexes in rats and thrombosis. Build a rat model of lower limb trauma, rats were divided into modeling cobra venom factor group (CVF group) and the control group, CVF group at different time points injection diluted cobra venom factor, the control group was injected with the same dose of PBS, and then under anesthesia, the two components at different time points blood, each of the periods of the total serum complement activity was measured after 120 hours, and 120h rats clotting index changes, another femoral vein anatomy all model rats, observed thrombosis. Rapid results the CVF serum complement activity was inhibited to normal levels below 10%, small fluctuations within the observation time, the complement activity of the control group, is maintained at more than 90%, no significant fluctuations. CVF group and the control group, PT, APTT significantly prolonged, the difference was statistically significant (P lt; 0.05), TT, FIB, change is inconsistent, the difference was not statistically significance (P gt; 0.05). CVF group thrombosis incidence of 20% thrombosis rate of 50% of the control group, the difference was statistically significant (P lt; 0.05) Conclusion complement consumption inhibition, to improve post-traumatic rat hypercoagulable state, effectively reducing the rat The incidence of deep vein thrombosis after trauma.

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