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Studies on the Flavanoids and Antioxidative Activities of the Leaves of Apocynum Venetum L.

Author: LiLiHong
Tutor: YuanZhong
School: Shenyang Pharmaceutical University
Course: Pharmacognosy
Keywords: Apocynum leaves Chemical composition Flavonoid glycosides Extraction Process Antioxidation
CLC: R285
Type: Master's thesis
Year: 2006
Downloads: 59
Quote: 1
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Abstract


Apocynum leaves Apocynaceae (Apocynaceae) plant Apocynum Apocynum ventum L. Dried leaves. The plant is rich in resources, widely distributed in Northwest China, North China, East China and northeastern provinces and autonomous regions. Commonly used to treat diseases such as nephritis, hypertension, neurasthenia. Apocynum leaves looking for its active ingredient, the study of the chemical composition. Apocynum leaves 95% ethanol, the water-soluble portion of the extract separated using a variety of chromatographic separation (silica gel column chromatography, a polyamide column chromatography, Sephadex LH-20 column chromatography, etc.) to obtain 11 compounds. Determined by physicochemical properties and spectral data analysis and other means of identification of the structure of the nine compounds as quercetin (quercetin, 1), isoquercitrin (isoquercitrin), hyperin (hyperoside, 3) quercetin-3-O-(6 \Quercetin-3-O-(6 \scopoletin, 9). Compound 5 and 8 for the first time from this genus were isolated. At the same time, the use of orthogonal design method, total flavonoids as index Apocynum leaves alcohol extraction process has been optimized. Orthogonal experiment results showed that the optimum extraction conditions of 50% ethanol, 10 times the amount of reflux extracted three times, each time 90min. The H 2 O 2 / Fe 2 system-generated model of hydroxyl radical and peroxynitrite anion (ONOO - ) radical model Apocynum leaf alcohol extract and share flavonoids free radical scavenging activity were measured. The results showed that all the samples have strong scavenging hydroxyl radicals; Apocynum leaves the main ingredient quercetin (quercetin, 1) and hyperin (hyperoside, 3) also cleared peroxynitrite nitrate anion (ONOO - ) the role of free radicals. Further, H 2 O 2 / Fe 2 system to produce hydroxyl radical induced mouse liver homogenate lipid peroxidation injury model, Apocynum leaves ethanol extract and its main ingredient quercetin (quercetin, 1) and hyperin (hyperoside, 3) Determination of the inhibitory effect of the results shows that the measured sample has a strong inhibition of lipid peroxidation role. The paper also Apocynum leaves herbal medicine research, the study of the chemical composition, pharmacological effects and clinical applications are reviewed.

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