Dissertation > Excellent graduate degree dissertation topics show

Study on the Bioactive Components from a Traditional Mongolian Medical Herb Clematis Aethusaefolia Turcz.

Author: FuMingHai
Tutor: BaoBaoQuan
School: Inner Mongolia University for Nationalities
Course: Monte Pharmacy
Keywords: Celery leaves clematis Biological activity Flavone C-glycosides
CLC: R29
Type: Master's thesis
Year: 2011
Downloads: 62
Quote: 0
Read: Download Dissertation

Abstract


Objective: The topics to Mongolia herbs parsley leaves clematis Clematis aethusaefolia dried aerial parts of Turcz study , extraction and separation select a valid active site of purified monomer compounds for the structural identification , and hope that the discovery of new compounds and bioactive a monomer compound. Method : take Turcz , Clematis aethusaefolia , clematis , celery leaves dried aerial parts of 10.6kg appropriate crushed , extracted with 75 % ethanol three times , concentrated alcohol solution to get bold material extract 1.53kg . The bold was extract add water suspension , washed with equal volumes of petroleum ether , chloroform , ethyl acetate , n-butanol and extracted three times the recovery solvent under reduced pressure . Obtained the different polarity extract, shrimp eggs lethal experimental determination of the biological activity of each of the extracted layer , select the effective parts , using a reverse phase silica gel column chromatography , thin layer chromatography , Sephadex LH -20 , semi-preparative HPLC method further separation and purification, to obtain a monomer compound . Check the purity of the melting point, high-performance liquid chromatography method , and the use of modern methods of 1H-NMR, 13C-NMR, HMBC, MS structural identification of compounds were obtained , and its discovery of new compounds and biological activity of single donor compound. Results: 11 compounds were obtained by separation of the ethyl acetate layer of the chemical composition of the extract of the the celery leaves clematis Clematis aethusaefolia Turcz effective active site , and its structure identification . The quercetin of determining the chemical structure of ferulic acid caffeic acid (1) (2) apigenin ( 3) luteolin biotin- 3'- 0- glucoside (4) -3-0 - glucoside ( 5) aliena the mesothelin the -3-0 - rhamnosyl (1-6 ) glucoside (6), kaempferol -3-0 - rhamnose - (1-6) - apigenin glucoside ( 7 ) -7-0 - ( P - coumaroyl ) apigenin glucoside ( 8 ) -6 - C -6 \butyl ester ( 11) . conclusions: The effective active site of celery leaves clematis clematis aethusaefolia Turcz ethyl acetate layer chemical composition , total of 11 compounds were isolated , and the use of 1H- NMR , 13C - NMR , HMBC, MS to determine the structure of the compound and the 11 compounds were first isolated from the plant . apigenin - 6 - C -6 \

Related Dissertations

  1. Research on Isolation and Purification of Activator Protein from Magnaporthe Grisea and Its Biological Activity Detection,S435.111.41
  2. Control Effects of Different Insecticides on Bemisia Tabaci(Gennadius) and Application Technology Study of Abamectin,S433
  3. Synthesis, Biological Activities and Potential Detoxification of N-(2,3,4,6-Tetra-O-Acetyl-β-D-Glucopyranosyl) Thiocarbamates,TQ450.21
  4. Phosphorus derivatives and pyrazole Triazolopyrimidine the Synthesis and Biological Activity of,TQ450.2
  5. Fusing Expression of Thioredoxin and Porcine IFNα and the Effect of Anti-PCV2,S852.65
  6. Novel isoxazoline and N- methyl- isoxazoline derivatives salt synthesis and biological activity of,O626
  7. Synthesis, Crystal Strucyures and Biological Actives of Vanadium Complexes with Schiff Base,O641.4
  8. Fermentation Culture and Metabolite Constituents of the Penicillium Verruculosum Yl-52 Isolated from the Rhizosphere of Stellera Chamaejasme L.,R284.1
  9. Colostrum PRPs features and functional studies,TS252.1
  10. Pichia Isolation, purification and biological activity,Q936
  11. TC4 alloy pulsed laser deposition of hydroxyapatite microstructure and properties of,TG146.23
  12. Medical magnesium alloy surface laser cladding Zr 60 Ti 6 Cu 19 Fe 5 Al 10 amorphous coating,TG174.44
  13. Plant extracts against Tetranychus cinnabarinus screening and biological activity of the active ingredient,S482.52
  14. Improvement Effects of Four Plantations for Sand-fixation on Soil Nutrient Contents and Biological Activities,S154
  15. Synthesis and Biological Activity of a Series of Berberine Compounds,S482.3
  16. Design、Synthesis and Bioactivity of Novel Strobilurin Fungicides,S482.2
  17. Study on Synthesis of Dipeptide and Glucose Glycopeptide by Phthalyl Anhydride,TQ450.1
  18. Bioinformatics Studies on hTNF-α,R341
  19. Study on Synthesis and Preliminary Biological Activity Tests of N-nitro Aryl Urea Derivatives,TQ450
  20. Effects of Continuous Planting on the Yield and Quality of Jerusalem Artichoke and Soil Properties,S632.9

CLC: > Medicine, health > Chinese Medicine > Chinese Minority Medicine
© 2012 www.DissertationTopic.Net  Mobile