Dissertation > Excellent graduate degree dissertation topics show

Preparation and Bioactivity of Protein-bound Polysaccharide from Cultural Medium of Ganoderma Sinensis

Author: WangLi
Tutor: HanBaoQin
School: Ocean University of China
Course: Biological Engineering
Keywords: Ganoderma sinensis protein-bound polysaccharide Fermentation Purification antitumor
CLC: R730.5
Type: Master's thesis
Year: 2011
Downloads: 40
Quote: 0
Read: Download Dissertation

Abstract


Cancer is a serious threat to human life, it’s a common and frequently-occurring disease. More than 10 million’s new cases and more than 6 million’s deaths occur each year in the world. The mortality rate is in second column, while cardiovascular disease is still stand in the top place.The present study has confirmed that Ganoderma polysaccharides Peptides have significant anti-angiogenesis activity of tumor cell growth also has a certain inhibitory effect, and relative to the polysaccharide, with oral effectiveness of the anti-tumor has a very bright future prospects. Ganoderma is endemic to China; few studies have reported it at home and abroad.This searching project further improves the studies of Ganoderma lucidum antitumor function on the basis of the previous studies. The deep fermentation prescription liquor for Ganoderma sinensis bacterial strains is optimized, and by one-factor-at-a-time methodology and orthogonal experiment, the optical deep liquid fermentation culture medium is determined （w/v） : glucose 3.5%, peptone 0.3%, yeast powder 0.1%, KH2PO4 0.1%, MgSO4 0.05%, vitamin B1 0.005%; The optimal fermentation cultivation condition is: 28°C, 160rpm/min, medium volume 150mL, strains grows best after 14 days, then hypha mycelium yield can reach 6.7 g/L. Removing the floating protein out of the fermentation liquor supernatant by two ways respectively. Different concentrations of TCA and （NH4）2SO4 salting-out results show that protein washout rate and polysaccharide loss rate are both positive relative with TCA and （NH4）2SO4。The protein elution rate will up to 70%, when the concentration of TCA reaches 10%. The results of SDS-PAGE are proving it indirectly. But, meanwhile the polysaccharide percentage loss will increase with protein elution rate growth. It appears a distinct red line on PAS-positive, showing that loss a lot of polysaccharide, over 25%. However, the （NH4）2SO4 （the concentration is 90%） could wash most of protein and control the loss of polysaccharide under 20%. It benefits for protein isolation process. The fermentation supernatant fluid by salting out use the concentration is 90% （NH4）2SO4,Throught the desalination, precede DEAE-Sepharose Fast Flow anion exchange chromatography, get the single sugar peak and single protein peak. In this case, the height of the suger peak and protein peak are superposition. Then, collect the coincidence peak, accquire the purified exocellular protein-bound polysaccharide （EP1）. This kind of complex has a obvious Anti-free radical activity and anti-tumour functions.

Related Dissertations

  1. Studies on Fermentation Optimization, Purification and Enzyme Characteristics of Lipase from Aspergillus Oryzae FS-1,TQ925.6
  2. Design, Synthesis and Biological Evaluation of Novel Podophyllotoxin Derivatives as Antitumor Agents,R284
  3. Optimization of Fermentation Conditions, Purification, Cloning and Expression of a Cold-active Lipase from Pseudomonas Sp.RT-1,TQ925
  4. Breeding of Fungal α-amylase High-producing Strain by Genome Shuffling,TQ925
  5. Study on the Breeding of High Efficient Detoxification Strains and Suggestion Parameters of Solid Fermentation on Cottonseed Meal,S816.6
  6. Screening of Bacterial Strains with High α-Galactosidase Activity and Its Gene Cloning, Expression, Purification and Characterization,TQ925
  7. Research on Biological Modification and Protection Activity of R-Phycoerythin from Porphyra Yezoensis,TS201.21
  8. Studies on Optimization Technology of Fermentation with Corn Straw and Cow Dung Mixed Material,S216.4
  9. Isolation and Identification of a Probiotic Strain Capable of Producing Glycerol and Optimization of Fermentation Conditions for the Strain,S823.5
  10. Novel Imidazophenanthroline Derivatives: Design, Synthesis and Evaluation of Biological Activity,TQ460.1
  11. Expression, Purification and Characterization of Trucated Type Recombinant Buckwheat Trypsin Inhibitor,R284.1
  12. Antitumor Effect and Expression of the Pro-apoptotic Bcl-2 Family Member N of Elemene in Mice Bearing Hepatocarcinoma H22 Cells-derived Tumor,R285.5
  13. Establishment of Subcutaneous Transplanted Tumor Model of Skin Adenocarcinoma in Balb/c Mice,R739.5
  14. 1, Bcl-2 small molecule inhibitors of protein synthesis and antitumor activity of two hydroxytyrosol new methods of synthesis,R96
  15. The Study of Structure and Bioactivity of the Polysaccharide Extracted from the Mycelium of Paecilomyces Hepiali,R284
  16. EOR properties of bacterial fermentation compatibility,TE357.9
  17. Fermentation Technology of Aspergillus Niger and Study of the Enzymatic Characteristics,TQ925.9
  18. Isolation and Purification of a Novel Antifungal Antibiotic and Optimization of Its Fermentation Conditions,TQ927
  19. The Study on Separation, Purification and Structure Analysis of the Polysaccharide from the Ligusticum Chuanxiong Hort,R284.1
  20. Isolation, Purification and Analysis of Polysaccharides from Bee Pollen of Rosa Rugosa,R285
  21. Multi- strain fermentation residue and yellow Whey protein feed production research,S816.4

CLC: > Medicine, health > Oncology > General issues > Tumor Therapy
© 2012 www.DissertationTopic.Net  Mobile