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Objective:By high-fat diet and injection of streptozotocin induced T2DM model rats,according to the blood glucose fluctuation of circadian rhythms to T2DM rats timingaerobic exercise intervention; through the changes before and after the intervention ofT2DM in rat kidney tissue sTM, AQP-2and serum related index content, effect ofaerobic exercise on the changes and the optional T2DM rat glomerular endothelial cellsand renal function when the concentration of urine.Methods:Clean and healthy male rats of4weeks80, weight180-220grams,1weeks afteradaptive feeding, were randomly divided into control group （group H, N=12） anddiabetic model group （group G, N=68）. Group H of conventional farming; group G4weeks high-sucrose and high-fat diet feeding after intraperitoneal injection ofstreptozotocin （1times/day x2）; group H was injected with citrate buffer solution.Modeling success （blood sugar content≥16.7mmol/L）, group G were randomlydivided into4groups: diabetic control group （C, N=14）, normal feeding withoutmovement;6:00exercise group （SM, N=14）,12:00group （SN, N=14） and18:00group （SE, N=14） for6weeks,0slope treadmill timing aerobic exercise intervention.Exercise intervention in12hours fasting blood plasma separation were tested, removalof renal tissue. The rats in each group were before modeling, after tail vein bloodmeasured fasting blood glucose, plasma insulin and ELISA method （FINS）, thrombinantithrombin complex （TAT）, vasopressin （AVP） levels and renal tissue of solublethrombomodulin（sTM）, the water channel protein-2（AQP-2）content.Use SPSS17.0software to process the data.Result:1. The blood glucose of health rats at18:00significantly higher than6:00（P<0.01），plasma insulin have no significant difference between18:00and6:00（P﹥ 0.05）.2. The level of blood glucose in T2DM rats was higher than normal （p <0.01）, asimilar result in the expression of insulin、TAT、sTM、AVPand AQP-2between the twogroups. No different was observed in level of blood glucose between the8:00and6:00.3. After six weeks of aerobic Chronoexercising, FPG level of S group （SM group,the SN group and SE group） were lower than C group （P <0.05）; the SN, SE groupwas lower than SM group（P <0.05）.Compared to the C groups, FINS of S（SM group,the SN group and SE group）were lower（P <0.05）, but no significant differencebetween the SM, SN, and SE group（P>0.05）.4. After six weeks of aerobic Chronoexercising, compare to the C group, Homa-IRin group S group decreased（P <0.01）, Homa-β of S group increased（P <0.01）,but theIAI have no change.5. After six weeks of aerobic Chronoexercising， the plasma TAT, sTM level inT2DM rats in S group compared with C group decreased（P<0.01）, and SE group wassignificantly lower than that in SM group, SN group（P<0.01）; plasma AVP, renalAQP-2level compared with S group C group decreased significantly（P<0.05）, Sgroups compared with SE group was higher than that of group SN（SM, P<0.05）.Conclusions:1. Blood glucose levels of normal rats with circadian rhythm; elevated bloodglucose in T2DM rats, the blood glucose fluctuation rhythm disorder, insulin resistance,blood hypercoagulability, glomerular endothelial cell damage, urinary concentratingfunction compensatory enhancement.2. The intervention of aerobic chronoexercising could improve insulin function,decrease the level of blood glucose, especially in the peak level, but there was on effecton the circadian rhythm.3. The intervention of aerobic chronoexercising could improve the polyuria indiabetic rats, reduce the loss of body water, especially in the peak level
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