Dissertation > Excellent graduate degree dissertation topics show

Chromosomal integration sites and histone methylation of HIV potential

Author: DingDongLin
Tutor: ZhuHuanZhang
School: Fudan University
Course: Biological Engineering
Keywords: HIV-1 HIV latent model intergration site CpG island methylation histone methylation
CLC: Q343.2
Type: Master's thesis
Year: 2011
Downloads: 1
Quote: 0
Read: Download Dissertation

Abstract


Acquired Immunodeficiency Syndrome (AIDS) caused by human immune-deficiency virus (HIV) infection is a kind of infectious diseases. Highly active antiretroviral therapy (HAART) can effectively suppress the replication of HIV and retard the progress of the disease in AIDS treatment. However, HAART can’t eradicate HIV completely because of the presence of latent reservoirs.Elucidating mechanism of latency contributes to interpret the formation of latent reservoirs and provide a strategy for HIV eradication. According to previous studies, HIV latency is associated with integrated site, CpG methylation of LTR, and histone methlation. We performed researches to elucidate the mechanism of latency.We used the models formulated previously to amply the human genomic sequence flanking to integration site. After sequencing, we pooled the data to blast in NCBI and obtained information of integration site. Secondly, By using bisulfate to convert genomic DNA, we analyzed CpG methlation in5’LTR to explain association between HIV latency and CpG methlation level. Finally, we performed ChIP to investigate levels of H3K9methylation of HIV-1LTR in latent cell lines.According to the researches, most integration sites locate in introns of transcriptional genes and integration may prefer active genes. After analyzing methylation of CpG island, we found hypomethylation in CpG island of all latent models. CpG island methylation possibly did not correlate with establishment of HIV-1latency. Besides the former investigations, MTA, a broad-spectrum histone methylation inhibitor, was used to reactivate latent cells. We found only in Clone11, latent HIV could be partially reactivated by MTA. Reactivation of latent HIV showed that histone methylation could contribute to latency. For further demonstration, ChIP was performed to test the role of histone methylation and level of H3K9. About2.27-fold decrease of H3K9me2level was obsrved in cells with MTA treating compared to cells without MTA. These results clearly showed that MTA reactivated HIV by decreasing level of histone methylation and HIV latency was associated with presence of H3K9me2.We suggested that dimethylation of H3K9which was dependent on the environment created by the site of integration, played a significant role in initial establishment of HIV-1latency rather than CpG methylation. Our work elucidated the possible mechanism of HIV latency, provided a target for reactivating latent HIV and a strategy for eradication of HIV latent reservoirs with antiviral drugs.

Related Dissertations

  1. HIV and Syphilis Infection and Risk Factors among Young Men Who Have Sex with Men,R512.91
  2. Exploration of Using CTA/CTB as Adjuvant for HIV-1 Regulatory/accessory Gene DNA Vaccine,R392
  3. A Research of the Incidence of HIV/HCV/HBV/syphilis and Risk Behaviors among Injecting Drug Users in Xichang County of Sichuan Province,R181.3
  4. The Epidemiological Study of HIV/STDS among Men Who Have Sex with Men in Beijing,R181.3
  5. Knowledge and Attitude about SEXUAL and HIV·AIDS among Students of Normal University,R193
  6. Study on the Synthetic Process of Amprenavir Intermediate,TQ463.5
  7. Study on the Function of Social Assistance by HIV Infector Organizations in Northeast Areas,R193
  8. Analysis of Investigation Result of Tuberculosis/HIV Co-infection in Changshu,R512.91
  9. Intervention Effective Evaluation on HIV-infector Detected among Tuberculosis Patients,R52
  10. The Effect of Health Belief on Condom Use Behavior among Female Sex Workers in Low-grade Areas,R181.3
  11. The Investigation of New HIV Infections and Related Behaviors on MSM in Wuhan 2008-2009,R512.91
  12. HIV-1 reverse transcriptase inhibitors QSAR and Molecular Design,R512.91
  13. Arsenic Induce Functional Re-expression of Estrogen Receptor α by Demethylation in Estrogen Receptor-negative Human Breast Cancer Cells,R737.9
  14. Estimation of HIV/AIDS in HeNan Province via Mathematical Model,R512.91
  15. Screening of Chinese Old World Monkey TRIMCyp Gene and Construction of Pseudotyped Human Immuno Defieiency Virus-l,Q93
  16. Studies on Biomimetic Synthesis of Hyperolactones and Biyouyanagin B,O643.32
  17. JMJD5 Proteolytically Processes Monomethylated Histone H3 N-tail,R730.2
  18. The Study on the Relationship between HBV X Gene Promoter Methylation in Liver Tissues and Clinical in Chronic Hepatitis B Patients,R512.62
  19. Shanxi Province, the HIV / AIDS infection and epidemic investigation and study of different populations of T lymphocyte subsets,R512.91
  20. Changes of Some Histone Modification during Oogenesis and Pre-Implantation Embryos Development in Mice,S813
  21. The Role of Methylation of p16、hMLH1 and BRAF Protein in Colon Carcinogenesis,R735.34

CLC: > Biological Sciences > Genetics > Genetics subdiscipline > Cytogenetic > Chromosome theory ( chromosome genetics)
© 2012 www.DissertationTopic.Net  Mobile