Dissertation > Excellent graduate degree dissertation topics show

Determination of Free Amino Acids in Three Kinds of Special Food in Shanxi by Liquid Chromatography and Liquid Chromatography Coupled to Mass Spectrometry

Author: CheLanLan
Tutor: LinQinBao
School: Shanxi University
Course: Drug analysis
Keywords: Asparagus Mature vinegar Jujube date Free amino acids Highperformance liquid chromatography(HPLC) Ultra-high performance liquidchromatography(UHPLC) Ultra-high performance liquid chromatographycoupled to mass spectrometry(UHPLC-MS/MS)
CLC: O657.63
Type: Master's thesis
Year: 2013
Downloads: 186
Quote: 0
Read: Download Dissertation

Abstract


Chapter1:The nutritive value in three kinds of special food in Shanxi, including asparagus, mature vinegar and jujube date, is introduced in brief. Tthe detection methods for free amino acids are summarized in detail. The research contents and innovations place in this paper are also proposed in this chapter.Chapter2:A high-performance liquid chromatography method for the determination of free amino acids in asparagus is presented. The sensitivity and accuracy were improved by optimizing the extracting condition. The detection limits are2.0-3.5μmol L-1and recoveries80%-119%. The developed method was applied to the determination of free amino acids in asparaguses of different colors, thickness and parts. It revealed that some free amino acids content is obviously different.Chapter3:An ultra-high performance liquid chromatography method for the determination of free amino acids in mature vinegar is presented. The sensitivity and accuracy were improved by optimizing chromatographic condition, pretreatment method and matrix effect. The detection limits are1.0mg L-1-9.0mg L-1and recoveries70%-109%. The developed method was applied to the detrmination of amino acids in ten kinds of mature vinegar. It revealed that the majority of free amino acids content in different manafactures is obviously different.Chapter4:An ultra-high performance liquid chromatography coupled to tandem mass spectrometry method for the determination of amino acids in Chinese jujube date is presented. The sensitivity and accuracy were improved by optimizing the mass condition, chromatographic condition, pretreatment method and matrix effect. The detection limits are0.8-6.0×102μg L-1and recoveries66%-107%. The developed method was applied to the determination of amino acids in four kinds of jujube date. It revealed that the majority of free amino acids content of different kinds is obviously different.

Related Dissertations

  1. Hot air drying characteristics of lettuce osmotic dehydration mass transfer kinetics and permeability,TS255.52
  2. The Response of Endogenous Phytohormones in Gracilaria Lemaneiformis under Environmental Stress,S968.4
  3. Changes of Free Amino Acids of Rat Skeletal Muscle and Serum after EIMD,R87
  4. The Study on Extraction of Asparagus Peel Flavonoid and Its Antioxidation Effect,TQ461
  5. Studies on Removal Efficiency of Nitrogen and Phosphorus of Eutrophic Seawater with "Steel Slag-Gracilaria Lemaneiformis" System,S917.3
  6. Studies on the Selection of Tea Species with High Theanine and the Caltivation Technology,S571.1
  7. Ecophysiology Compensatory Mechanisms of Caragana Korshinskii after Clipping,S793
  8. Study on Mutagentic Effects of Space Environment and Biological Changes of Potential Mutants on the SP1 Generation of Dianthus Barbatus L.,S681.5
  9. The Chemical Degradation of Defective Gracilaria Lemaneifromis Polysaccharide and the Protective Effect on Diabetic Mice,R285
  10. Studies on the Extraction, Purification and Hemocytolysis of Asparagus Saponins,TQ464
  11. In the grass carp (Ctenopharyngodon idellus) feed supplement microcapsules and crystal Amino Acids,S963
  12. The Research on Export-oriented Agricultural Development in Yuncheng City,F327
  13. Asparagus synthetic agar cloning and expression of genes related to regulation of,S968.434
  14. Theoretical and Experimental Investigation on the High Pressure Treatment of Food,TS205
  15. Studies on Biological Comparisons and Hybridization of Two Geographic Populations of Coelomactra Antiquata,S917.4
  16. Asparagus (Gracilaria lemaneiformis) Screening of microsatellite markers polymorphism between strains,S917.3
  17. Studies on the Cloning and Analysis of Galactose-1-phosphate Uridylyltransferase Gene of Gracilariopsis Lemaneiformis and the Correlation between Gene Expression and Agar Synthesis,S917.3
  18. Technical Study of Vacuum Fried Salicornia Bigelovii Torr.,TS255.3
  19. Study on Drying Technology of Salicornia Bigelovii Torr.,TS255.5
  20. Sludge Acclimation of Microbiological Treatment of Hypersaline Refractory Waste Water,X703
  21. A Study on Ecological Pattern of "The Asparagus and Forest-Grass" to Rehabilitate Wind-Sandy Wasteland in Southern China,X171.1

CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Mass Spectrometry
© 2012 www.DissertationTopic.Net  Mobile