Dissertation > Excellent graduate degree dissertation topics show

Studies on the Fermentation Conditions of Coenzyme Q10 Production by Saccharomycete

Author: ZhaoShuQuan
Tutor: JiangGuoTuo
School: Dalian University of Technology
Course: Of Food Science
Keywords: Coenzyme Q10 Saccharomyce Selection Cultural condition Isolationand purification
CLC: TQ464.8
Type: Master's thesis
Year: 2009
Downloads: 1
Quote: 0
Read: Download Dissertation

Abstract


The Coenzyme Ql0is a prenylated benzoquinone lipid, and it is also named ubiquinone.The chemical name is2,3-dimethoxy-5-methyl-6-polyprenyl-1,4-benzoquinone. Members ofthe Coenzyme Ql0are found in microorganisms, plants, and the heart, kidney, liver of animal,where they function are redox carriers in mitochondria or in association with respiratoryprocesses of electron transport chains. It is an antioxidant produced naturally in the cells.TheCoenzyme Q10exhibited excellent medical and physiological activities against variousdiseases such as heart diseases, cancer, hepatitis and so on.Mutagenesis and screening of mutant strains with high yield of Coenzyme Ql0，optimization of fermentation conditions，extraction and determination of Coenzyme Ql0werestudied in this text. Saccharomyces cerevisiae FCC2146were used in the fermentation of coenzyme Q10production at the first time.Experiment to determine the acid-acetone as a separation and extraction method ofcoenzyme Q10, it is simple equipment, energy consumption and low cost, and also lessdestructive to the coenzyme Q10.At the same time, the production of coenzyme Q10wasidentificated and determinated by combining of Thin-layer Chromatography (TLC) and HighPerformance Liquid Chromatography (HPLC).Saccharomyces cerevisiae FCC2146were investigated from five different speciesidentified in the Blastocystis,and its dry weight of thalli was4.67g/L、the yield of intrallularCoQl0was1.239mg/g and coenzyme Q10production was5.785mg/L.Optimize fermentation conditions and culture media for CoQl0production by ResponseSurface Methodology,the results showed that Glucose concentration was1.95%,yeast powder＋soy peptone(1:5) concentration was0.483%,2.55%carrot juice was added as growthfactor,initial pH was6.0,the medium volume was50mL in250mL Erlenmeyer flask, inocul-um concentration was7%, the fermentation temperature was30℃，the fermentation time was48h,the yield of CoQl0reached16.4mg/L,which was183％higher than that beforeoptimization．

Related Dissertations

  1. Research on Graph-Based Algorithm for Tagsnps Selection,Q78
  2. The Research on the Architectural Adumbral Method of Building,TU226
  3. Feature Extraction, Selection and Combination in Lipreading,TP391.41
  4. Functional Realization of the Competitive Selection System,D262.3
  5. The Influence of the Primary School Class Cadres’ Selection Mode on the Students’ Democratic Participation Ability,G625.1
  6. Preliminary Study of CoenzymeQ10 on Renal Protection to Avian Gout,S858.31
  7. Improving of Artificial Imune Classification and Anomaly Detection Algorithms,R392.1
  8. The Software Testing Technique Research of the ECDIS Based on Program Slicing,TP311.53
  9. Study on Selection and Allocation of Agricultural Machinery in Huanghai State Farm,S232.3
  10. The Workshop Process Design of the Soy Poly-peptides,TS214.2
  11. Chongqing township cadres competency modeling and application research,D630
  12. Body Sensor Networks Adaptive Communication Protocol,TP212.9
  13. Bayesian methods under two ordinal value of multi-value data model with simultaneous identification of outliers,O212.1
  14. SAR interferometric method for optimal selection,P225.2
  15. Research on Intrusion Detection Based on Feature Selection,TP393.08
  16. Research on Supplier-Selection Based on Strategic Sourcing,F426.31
  17. Research on Micro Simulation of Urban Road Guide Signs and It’s Realization,U491.52
  18. Ammonia-N Stress on Misgurnus Anguillicaudatus and Second Generation Population of Mass Selection,S966.4
  19. Research on LEACH-based Clustering Routing Protocol in Sensor Networks,TP212.9
  20. Model Selection for Longitudinal Data Based on the Quadratic Inference Function under a Simple Order Restriction,O212.1
  21. Effects of Reproductive Strategy Choice on Reproductive Fitness in Eurasian Nuchatch,Q958

CLC: > Industrial Technology > Chemical Industry > Pharmaceutical chemical industry > Drug production of biological products > Enzyme and coenzyme
© 2012 www.DissertationTopic.Net  Mobile