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In this study, by national validation of the suitability of the Yangtze River valley in 2008 planted transgenic insect-resistant hybrid cotton varieties in cotton two parents of the 66 (female parent: 9018; paternal: CCRI 45) hybridized to construct F2 : 3 segregating population. F2: 3 families SSR molecular markers to construct genetic linkage map, the composite interval method (CIM) and mixed linear model based QTLNetwork2.0 software QTL mapping and epistasis analysis of yield and fiber quality traits. From the molecular level to explore the breeding method and path, laid the foundation for the cotton molecular breeding. In this study, the use of different sources of parental filter 6753 pairs of SSR primers, 125 polymorphic primers. The the polymorphic primers detector (9018 × CCRI 45) F2; 3 groups of 219 per plant marker genotypes, a total of 193 marker loci. Genetic map construction software Joinmap 3.0, 193 SSR markers for genetic linkage analysis, 127 markers was constructed 33 linkage groups, covering a total 1396.2cM, equivalent to 31.0% of the cotton genome, the longest linkage group was 96.2 cM, containing 13 markers, the shortest 13.8cM, contains only two markers between markers the average distance 10.99cM, and the remaining 66 marker loci can not be allocated to linkage group. Reference predecessors to build the genetic linkage map, 33 linkage groups were localized on chromosome 20. Composite interval mapping method (CIM) WindowsQTLCartographer 2.5 software, fiber quality and yield trait QTL mapping in two environments, and detected a total of 10 fiber quality traits and 10 QTLs for yield traits, including plant height 1 months, a lint percentage, lint index a boll weight 3, before frost seed cotton weight of two cream seed cotton weighs a a district total output, three fiber length, fiber uniformity of a micmnaire value of 2, a fiber elongation, fiber strength 3. There are two QTLs for more than the threshold determined by permutation test for significant QTLs. Two environments found no common QTL. Analyzed using mixed linear model-based composite interval mapping method, additive effect QTLs detected seven fiber quality and yield traits and which two fiber quality, yield traits 5. Detected 13 pairs plus × plus bit QTLs, three pairs of fiber quality traits and yield traits. A single locus additive QTL and five pairs of epistatic QTL and environment interaction effects. In the two detection methods detected 15 yields and components of QTL 9 QTL allele from parents of high value. Fiber quality aspects, the two detection methods detected 12 fiber quality QTL, the eight QTL allele from parents of high value. These results further QTL fine mapping, as well as cotton heterosis use laid the foundation and can be part of the material for the follow-up study to use this reference.
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