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In this paper, carcinogenic heterocyclic aromatic amines (HAAs) are difficult to measure since only trace levels are present in processed meat products. Typical heterocyclic aromatic amines, including2-amino-3-methylimidazo [4,5-f] quinoline (IQ),2-amino-3,4-dimethylimidazo [4,5-f] quinoline (MeIQ),2-amino-3,8-dimethyli-midazo [4,5-f] quinoxaline (MeIQx),2-amino-3,4,8-trimethylimi-dazo [4,5-f] quinoxaline (4,8-DiMeIQx),2-amino-l-methyl-6-ph-enylimidazo [4,5-b]pyridine (PhIP) were studied with2-Amino-3,4,7,8-dimethylimidazo [4,5-f] quinoxalline (TriMeIQx) as an internal standard. Matrix solid-phase extraction was employed to extract heterocyclic aromatic amines from meat samples and the analytes were purified and enriched using tandem solid phase extraction (SPE), with siliprep propylsulfonic acid (Si-SCX-2) coupled to a C18cartridge. Using ion-pair compound and an internal standard for quanlitative and quantitative analysis, developed a sensitive and accurate method for their rapid quantification in animal-derived products.Liquid chromatography-electrospray-tandem mass spectrometry (LC-ESI-MS/MS) conditions were analyzed to enhance detection sensitivity. A Zorbax Eclipse Plus C18column (2.1X150mm,3.5-Micron; Agilent) was choosen to separated the analytes using a mobile phase of pH3.510mmol L-1ammonium formate (A) and acetonitrile (B) with the gradient elute program, all analytes were baseline separated with better peak symmetry. The results showed that good linearity was obtained over the range of0.05-500μg L-1with r2>0.9995, the limits of detection for each analyte in the standard solutions were0.08-0.3pg.The conditions of matrix solid-phase extraction (MSPD) and accelerated solvent extraction (ASE) were also optimized to extract HAAs and compared the extraction efficiency. A number of parameters of tandem SPE methods (Si-SCX-2coupled to C18), including pH, eluent and its volume, were carefully optimized to improve the purification efficiency. The established method was applied to evaluate commercial meat products. At three spiked levels of0.2,1.4μg kg-1, the recoveries and relative standard deviations were measured as76.4-122.2%and0.9-23.4%, respectively, the limits of detection for each analyte in the meat matrix were0.1μg kg-1,suggesting the developed method is promising for the accurate quantification of heterocyclic aromatic amines at trace levels in processed meats.
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